Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice
Introduction: Cholesterol gallstone (CGS) is a biliary tract disorder requiring treatment in approximately 20% of patients. The efficacy of Chaihu Shugan in preventing CGS recurrence after successful treatment remains uncertain.Methods: We examined the in vivo preventive efficacy of Chaihu Shugan us...
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1291236/full |
| _version_ | 1827367638639050752 |
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| author | Wei Wang Kun Zhang Bin Liu Tong Zhou Yu Tang Yuliang Li |
| author_facet | Wei Wang Kun Zhang Bin Liu Tong Zhou Yu Tang Yuliang Li |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Introduction: Cholesterol gallstone (CGS) is a biliary tract disorder requiring treatment in approximately 20% of patients. The efficacy of Chaihu Shugan in preventing CGS recurrence after successful treatment remains uncertain.Methods: We examined the in vivo preventive efficacy of Chaihu Shugan using a CGS mouse model and used multi-omics to study the interplay between gut microbiota, metabolism, and gene expression.Results: The intestinal microbiota was severely dysregulated during the formation of CGS, showing a marked decrease in the abundance of beneficial microbiota, especially Lactobacillus and Akkermansia. Chaihu Shugan prevented CGS formation by restoring the composition of the gut microbiota and reversing the metabolic disturbances caused by dysbiosis. This preventive effect of Chaihu Shugan was paralleled by changes in the expression of metabolism-related genes in the liver. A network pharmacology analysis of Chaihu Shugan revealed that obacunone may be the key active metabolite in regulating bile acid metabolism. Multi-omics and correlation analyses elucidated the interplay between gut microbiota, metabolism, and gene alterations in the dose-dependent effect of Chaihu Shugan.Conclusion: Our data show that Chaihu Shugan can prevent CGS and indicate its mechanisms of action. |
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| format | Article |
| id | doaj.art-3dbdb9f0d42b4fd9b03336a9174ff9ec |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-08T09:15:58Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-3dbdb9f0d42b4fd9b03336a9174ff9ec2024-01-31T17:28:58ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-01-011410.3389/fphar.2023.12912361291236Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in miceWei Wang0Kun Zhang1Bin LiuTong Zhou2Yu Tang3Yuliang Li4Department of Intervention, The Second Hospital of Shandong University, Jinan, ChinaShanghai Biotree Biotech Co., Ltd, Shanghai, ChinaDepartment of Intervention, The Second Hospital of Shandong University, Jinan, ChinaDepartment of Geriatrics, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Intervention, The Second Hospital of Shandong University, Jinan, ChinaIntroduction: Cholesterol gallstone (CGS) is a biliary tract disorder requiring treatment in approximately 20% of patients. The efficacy of Chaihu Shugan in preventing CGS recurrence after successful treatment remains uncertain.Methods: We examined the in vivo preventive efficacy of Chaihu Shugan using a CGS mouse model and used multi-omics to study the interplay between gut microbiota, metabolism, and gene expression.Results: The intestinal microbiota was severely dysregulated during the formation of CGS, showing a marked decrease in the abundance of beneficial microbiota, especially Lactobacillus and Akkermansia. Chaihu Shugan prevented CGS formation by restoring the composition of the gut microbiota and reversing the metabolic disturbances caused by dysbiosis. This preventive effect of Chaihu Shugan was paralleled by changes in the expression of metabolism-related genes in the liver. A network pharmacology analysis of Chaihu Shugan revealed that obacunone may be the key active metabolite in regulating bile acid metabolism. Multi-omics and correlation analyses elucidated the interplay between gut microbiota, metabolism, and gene alterations in the dose-dependent effect of Chaihu Shugan.Conclusion: Our data show that Chaihu Shugan can prevent CGS and indicate its mechanisms of action.https://www.frontiersin.org/articles/10.3389/fphar.2023.1291236/fullcholesterol gallstoneChaihu Shuganobacunonegut microbiotagut metabolism |
| spellingShingle | Wei Wang Kun Zhang Bin Liu Tong Zhou Yu Tang Yuliang Li Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice Frontiers in Pharmacology cholesterol gallstone Chaihu Shugan obacunone gut microbiota gut metabolism |
| title | Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice |
| title_full | Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice |
| title_fullStr | Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice |
| title_full_unstemmed | Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice |
| title_short | Chaihu Shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice |
| title_sort | chaihu shugan prevents cholesterol gallstone formation by ameliorating the microbiota dysbiosis and metabolic disturbance in mice |
| topic | cholesterol gallstone Chaihu Shugan obacunone gut microbiota gut metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1291236/full |
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