ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells
The novel zinc finger protein 121 (ZNF121) has been demonstrated to physically and functionally associate with the MYC oncoprotein to regulate cell proliferation and likely breast cancer development. To further understand how ZNF121 functions in cell proliferation and carcinogenesis, we identified a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-12-01
|
| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12530 |
| _version_ | 1797870996854669312 |
|---|---|
| author | Ang Luo Kailun Zhang Yanxia Zhao Zhengmao Zhu Liya Fu Jin‐Tang Dong |
| author_facet | Ang Luo Kailun Zhang Yanxia Zhao Zhengmao Zhu Liya Fu Jin‐Tang Dong |
| author_sort | Ang Luo |
| collection | DOAJ |
| description | The novel zinc finger protein 121 (ZNF121) has been demonstrated to physically and functionally associate with the MYC oncoprotein to regulate cell proliferation and likely breast cancer development. To further understand how ZNF121 functions in cell proliferation and carcinogenesis, we identified and characterized the interaction of ZNF121 with zinc finger and BRCA1‐interacting protein with a KRAB domain 1 (ZBRK1), a breast and ovarian cancer susceptibility protein 1 (BRCA1)‐interacting protein, using the yeast two‐hybrid assay and other approaches. We also found that ZNF121 bound to BRCA1. Functionally, ZFN121 suppressed the expression of ANG1 and HMGA2, two common downstream targets of ZBRK1 and BRCA1. Interestingly, ZNF121 also regulated the expression of BRCA1 and ZBRK1. These findings suggest that ZNF121 is likely a member of the BRCA1/CtIP/ZBRK1 repressor complex that plays a role in breast cancer. |
| first_indexed | 2024-04-10T00:37:15Z |
| format | Article |
| id | doaj.art-3dbdc0b23ae1485198e8e5384f4be506 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-10T00:37:15Z |
| publishDate | 2018-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-3dbdc0b23ae1485198e8e5384f4be5062023-03-14T13:06:01ZengWileyFEBS Open Bio2211-54632018-12-018121943195210.1002/2211-5463.12530ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cellsAng Luo0Kailun Zhang1Yanxia Zhao2Zhengmao Zhu3Liya Fu4Jin‐Tang Dong5Department of Genetics and Cell Biology Nankai University College of Life Sciences Tianjin ChinaDepartment of Genetics and Cell Biology Nankai University College of Life Sciences Tianjin ChinaDepartment of Genetics and Cell Biology Nankai University College of Life Sciences Tianjin ChinaDepartment of Genetics and Cell Biology Nankai University College of Life Sciences Tianjin ChinaDepartment of Genetics and Cell Biology Nankai University College of Life Sciences Tianjin ChinaDepartment of Genetics and Cell Biology Nankai University College of Life Sciences Tianjin ChinaThe novel zinc finger protein 121 (ZNF121) has been demonstrated to physically and functionally associate with the MYC oncoprotein to regulate cell proliferation and likely breast cancer development. To further understand how ZNF121 functions in cell proliferation and carcinogenesis, we identified and characterized the interaction of ZNF121 with zinc finger and BRCA1‐interacting protein with a KRAB domain 1 (ZBRK1), a breast and ovarian cancer susceptibility protein 1 (BRCA1)‐interacting protein, using the yeast two‐hybrid assay and other approaches. We also found that ZNF121 bound to BRCA1. Functionally, ZFN121 suppressed the expression of ANG1 and HMGA2, two common downstream targets of ZBRK1 and BRCA1. Interestingly, ZNF121 also regulated the expression of BRCA1 and ZBRK1. These findings suggest that ZNF121 is likely a member of the BRCA1/CtIP/ZBRK1 repressor complex that plays a role in breast cancer.https://doi.org/10.1002/2211-5463.12530BRCA1ZBRK1ZNF121 |
| spellingShingle | Ang Luo Kailun Zhang Yanxia Zhao Zhengmao Zhu Liya Fu Jin‐Tang Dong ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells FEBS Open Bio BRCA1 ZBRK1 ZNF121 |
| title | ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells |
| title_full | ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells |
| title_fullStr | ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells |
| title_full_unstemmed | ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells |
| title_short | ZNF121 interacts with ZBRK1 and BRCA1 to regulate their target genes in mammary epithelial cells |
| title_sort | znf121 interacts with zbrk1 and brca1 to regulate their target genes in mammary epithelial cells |
| topic | BRCA1 ZBRK1 ZNF121 |
| url | https://doi.org/10.1002/2211-5463.12530 |
| work_keys_str_mv | AT angluo znf121interactswithzbrk1andbrca1toregulatetheirtargetgenesinmammaryepithelialcells AT kailunzhang znf121interactswithzbrk1andbrca1toregulatetheirtargetgenesinmammaryepithelialcells AT yanxiazhao znf121interactswithzbrk1andbrca1toregulatetheirtargetgenesinmammaryepithelialcells AT zhengmaozhu znf121interactswithzbrk1andbrca1toregulatetheirtargetgenesinmammaryepithelialcells AT liyafu znf121interactswithzbrk1andbrca1toregulatetheirtargetgenesinmammaryepithelialcells AT jintangdong znf121interactswithzbrk1andbrca1toregulatetheirtargetgenesinmammaryepithelialcells |