Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori
Helicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-inflammatory cytokines. Polymorphisms in genes that code cytokines influence cytokine secretion levels and appear to contribute to the risk of gastric diseases. In this sense, we performed this study to...
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Elsevier
2017-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118215007215 |
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| author | Ivy Bastos Ramis Júlia Silveira Vianna Carla Vitola Gonçalves Andrea von Groll Odir Antônio Dellagostin Pedro Eduardo Almeida da Silva |
| author_facet | Ivy Bastos Ramis Júlia Silveira Vianna Carla Vitola Gonçalves Andrea von Groll Odir Antônio Dellagostin Pedro Eduardo Almeida da Silva |
| author_sort | Ivy Bastos Ramis |
| collection | DOAJ |
| description | Helicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-inflammatory cytokines. Polymorphisms in genes that code cytokines influence cytokine secretion levels and appear to contribute to the risk of gastric diseases. In this sense, we performed this study to identify the polymorphisms in the IL-6, IL-8, and IL-10 genes and their associations with H. pylori infection and gastric pathologies.
Methods: Gastric biopsy samples of 151 patients infected with H. pylori and 76 uninfected individuals were used. Helicobacter pylori infection was diagnosed by histological examination and the detection of the ureA and glmM genes. The polymorphisms in the IL-6 (at position −174), IL-8 (at position −251), and IL-10 (at position −819) were detected by polymerase chain reaction–restriction fragment length polymorphism.
Results: Among the genetic polymorphisms studied, we observed that only the presence of the A allele at position −251 of the IL-8 gene was significantly associated with H. pylori infection. In addition, patient carriers of the A/A genotype at position −251 of the IL-8 gene and carriers of the T allele at position −819 of the IL-10 gene had an increased risk of peptic ulcer disease in the presence of H. pylori infection. We did not find a correlation between polymorphisms in the IL-6, IL-8, and IL-10 genes and a higher risk of gastric carcinoma.
Conclusion: We demonstrated that polymorphisms in the IL-8 gene was significantly associated with H. pylori infection. Furthermore, polymorphisms in the IL-8 and IL-10 genes were associated with an enhanced risk of peptic ulcer disease in H. pylori-positive patients. |
| first_indexed | 2024-12-14T08:09:10Z |
| format | Article |
| id | doaj.art-3dbfc2b6aafe4ee7959b8ed2f0432b35 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-14T08:09:10Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-3dbfc2b6aafe4ee7959b8ed2f0432b352022-12-21T23:10:06ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822017-04-0150215315910.1016/j.jmii.2015.03.002Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pyloriIvy Bastos Ramis0Júlia Silveira Vianna1Carla Vitola Gonçalves2Andrea von Groll3Odir Antônio Dellagostin4Pedro Eduardo Almeida da Silva5Centro de Desenvolvimento Tecnológico–Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, BrazilCentro de Desenvolvimento Tecnológico–Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, BrazilNúcleo de Pesquisa em Microbiologia Médica–Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, BrazilNúcleo de Pesquisa em Microbiologia Médica–Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, BrazilCentro de Desenvolvimento Tecnológico–Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, BrazilCentro de Desenvolvimento Tecnológico–Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, BrazilHelicobacter pylori-induced gastric mucosal inflammation is mediated by proinflammatory and anti-inflammatory cytokines. Polymorphisms in genes that code cytokines influence cytokine secretion levels and appear to contribute to the risk of gastric diseases. In this sense, we performed this study to identify the polymorphisms in the IL-6, IL-8, and IL-10 genes and their associations with H. pylori infection and gastric pathologies. Methods: Gastric biopsy samples of 151 patients infected with H. pylori and 76 uninfected individuals were used. Helicobacter pylori infection was diagnosed by histological examination and the detection of the ureA and glmM genes. The polymorphisms in the IL-6 (at position −174), IL-8 (at position −251), and IL-10 (at position −819) were detected by polymerase chain reaction–restriction fragment length polymorphism. Results: Among the genetic polymorphisms studied, we observed that only the presence of the A allele at position −251 of the IL-8 gene was significantly associated with H. pylori infection. In addition, patient carriers of the A/A genotype at position −251 of the IL-8 gene and carriers of the T allele at position −819 of the IL-10 gene had an increased risk of peptic ulcer disease in the presence of H. pylori infection. We did not find a correlation between polymorphisms in the IL-6, IL-8, and IL-10 genes and a higher risk of gastric carcinoma. Conclusion: We demonstrated that polymorphisms in the IL-8 gene was significantly associated with H. pylori infection. Furthermore, polymorphisms in the IL-8 and IL-10 genes were associated with an enhanced risk of peptic ulcer disease in H. pylori-positive patients.http://www.sciencedirect.com/science/article/pii/S1684118215007215Genetic polymorphismHelicobacter pyloriInterleukin-6 geneInterleukin-8 geneInterleukin-10 gene |
| spellingShingle | Ivy Bastos Ramis Júlia Silveira Vianna Carla Vitola Gonçalves Andrea von Groll Odir Antônio Dellagostin Pedro Eduardo Almeida da Silva Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori Journal of Microbiology, Immunology and Infection Genetic polymorphism Helicobacter pylori Interleukin-6 gene Interleukin-8 gene Interleukin-10 gene |
| title | Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori |
| title_full | Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori |
| title_fullStr | Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori |
| title_full_unstemmed | Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori |
| title_short | Polymorphisms of the IL-6, IL-8 and IL-10 genes and the risk of gastric pathology in patients infected with Helicobacter pylori |
| title_sort | polymorphisms of the il 6 il 8 and il 10 genes and the risk of gastric pathology in patients infected with helicobacter pylori |
| topic | Genetic polymorphism Helicobacter pylori Interleukin-6 gene Interleukin-8 gene Interleukin-10 gene |
| url | http://www.sciencedirect.com/science/article/pii/S1684118215007215 |
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