Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy
Two types of inclusion bodies were consistently observed under light microscopy in bean (Phaseolus vulgaris) leaf tissue infected with bean rugose mosaic virus (BRMV), a species of the genus Comovirus, family Comoviridae. One type consisted of vacuolated inclusions found mainly in the cytoplasm of e...
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| Format: | Article |
| Language: | English |
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Vicerractoría Investigación
2000-12-01
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| Series: | Revista de Biología Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442000000400019 |
| _version_ | 1797727336454422528 |
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| author | Carmen Rivera Lisela Moreira Carmona |
| author_facet | Carmen Rivera Lisela Moreira Carmona |
| author_sort | Carmen Rivera |
| collection | DOAJ |
| description | Two types of inclusion bodies were consistently observed under light microscopy in bean (Phaseolus vulgaris) leaf tissue infected with bean rugose mosaic virus (BRMV), a species of the genus Comovirus, family Comoviridae. One type consisted of vacuolated inclusions found mainly in the cytoplasm of epidermal cells. The other type consisted of abundant crystalloid inclusions of different sizes and shapes found consistently in glandular hairs, guard cells, phloem tissue, xylem elements and occasionally in epidermal and mesophyll tissues. The two types of inclusion bodies stained with Azure A and Luxol Brilliant Green Bl-Calcomine Orange 2RS (O-G), and were similar to those seen to be caused by other species of comoviruses.<br>Se observaron dos tipos de inclusiones virales, mediante microscopia de luz, en hojas de plantas de frijol (Phaseolus vulgaris) previamente infectadas con el virus del mosaico rugoso del frijol ("bean rugose mosaic comovirus", BRMV), especie del género Comovirus, familia Comoviridae. Se hallaron inclusiones vesiculadas, principalmente en el citoplasma de células de la epidermis, y abundantes inclusiones cristalinas de diferentes formas y tamaños siempre en células guarda, tricomas glandulares, floema, elementos del xilema y ocasionalmente en células epidérmicas y del mesófilo. Ambos tipos de inclusiones tiñeron con Azure A y con la tinción, verde naranja (Luxol Brilliant Green BL-Calcomine Orange 2 RS) conocida como OG, y son similares a las inclusiones inducidas por otras especies del género Comovirus. |
| first_indexed | 2024-03-12T10:58:22Z |
| format | Article |
| id | doaj.art-3dc2094bac6c4961a2db85ac4536cda0 |
| institution | Directory Open Access Journal |
| issn | 0034-7744 2215-2075 |
| language | English |
| last_indexed | 2024-03-12T10:58:22Z |
| publishDate | 2000-12-01 |
| publisher | Vicerractoría Investigación |
| record_format | Article |
| series | Revista de Biología Tropical |
| spelling | doaj.art-3dc2094bac6c4961a2db85ac4536cda02023-09-02T06:02:46ZengVicerractoría InvestigaciónRevista de Biología Tropical0034-77442215-20752000-12-01484955959Inclusion bodies induced by bean rugose mosaic virus seen under light microscopyCarmen RiveraLisela Moreira CarmonaTwo types of inclusion bodies were consistently observed under light microscopy in bean (Phaseolus vulgaris) leaf tissue infected with bean rugose mosaic virus (BRMV), a species of the genus Comovirus, family Comoviridae. One type consisted of vacuolated inclusions found mainly in the cytoplasm of epidermal cells. The other type consisted of abundant crystalloid inclusions of different sizes and shapes found consistently in glandular hairs, guard cells, phloem tissue, xylem elements and occasionally in epidermal and mesophyll tissues. The two types of inclusion bodies stained with Azure A and Luxol Brilliant Green Bl-Calcomine Orange 2RS (O-G), and were similar to those seen to be caused by other species of comoviruses.<br>Se observaron dos tipos de inclusiones virales, mediante microscopia de luz, en hojas de plantas de frijol (Phaseolus vulgaris) previamente infectadas con el virus del mosaico rugoso del frijol ("bean rugose mosaic comovirus", BRMV), especie del género Comovirus, familia Comoviridae. Se hallaron inclusiones vesiculadas, principalmente en el citoplasma de células de la epidermis, y abundantes inclusiones cristalinas de diferentes formas y tamaños siempre en células guarda, tricomas glandulares, floema, elementos del xilema y ocasionalmente en células epidérmicas y del mesófilo. Ambos tipos de inclusiones tiñeron con Azure A y con la tinción, verde naranja (Luxol Brilliant Green BL-Calcomine Orange 2 RS) conocida como OG, y son similares a las inclusiones inducidas por otras especies del género Comovirus.http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442000000400019Comoviruscytopathologylight microscopydiagnosisbean |
| spellingShingle | Carmen Rivera Lisela Moreira Carmona Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy Revista de Biología Tropical Comovirus cytopathology light microscopy diagnosis bean |
| title | Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy |
| title_full | Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy |
| title_fullStr | Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy |
| title_full_unstemmed | Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy |
| title_short | Inclusion bodies induced by bean rugose mosaic virus seen under light microscopy |
| title_sort | inclusion bodies induced by bean rugose mosaic virus seen under light microscopy |
| topic | Comovirus cytopathology light microscopy diagnosis bean |
| url | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442000000400019 |
| work_keys_str_mv | AT carmenrivera inclusionbodiesinducedbybeanrugosemosaicvirusseenunderlightmicroscopy AT liselamoreiracarmona inclusionbodiesinducedbybeanrugosemosaicvirusseenunderlightmicroscopy |