Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central Chile
The diversity of orchid mycorrhizal fungi (OMF) and other beneficial root-associated fungi in temperate forests has scarcely been examined. This study aimed to analyze the diversity of mycorrhizal and rhizosphere-associated fungal communities in the terrestrial orchids <i>Gavilea lutea</i&g...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/8/794 |
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| author | Héctor Herrera Tedy Sanhueza Rafael Borges da Silva Valadares Francisco Matus Guillermo Pereira Cristian Atala María de la Luz Mora Cesar Arriagada |
| author_facet | Héctor Herrera Tedy Sanhueza Rafael Borges da Silva Valadares Francisco Matus Guillermo Pereira Cristian Atala María de la Luz Mora Cesar Arriagada |
| author_sort | Héctor Herrera |
| collection | DOAJ |
| description | The diversity of orchid mycorrhizal fungi (OMF) and other beneficial root-associated fungi in temperate forests has scarcely been examined. This study aimed to analyze the diversity of mycorrhizal and rhizosphere-associated fungal communities in the terrestrial orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> growing in high-orchid-population-density areas in the piedmont of the Andes Cordillera with native forest (<i>Nothofagus-Araucaria</i>) and Coastal Cordillera with an exotic plantation (<i>Pinus-Eucalyptus</i>) in south-central Chile. We focused on rhizosphere-inhabiting and peloton-associated OMF in a native forest (Andes Cordillera) and a mixed forest (Coastal Cordillera). The native terrestrial orchids <i>G. lutea</i> and <i>C. collicensis</i> were localized, mycorrhizal root segments were taken to isolate peloton-associated OMF, and rhizosphere soil was taken to perform the metabarcoding approach. The results revealed that Basidiomycota and Ascomycota were the main rhizosphere-inhabiting fungal phyla, showing significant differences in the composition of fungal communities in both sites. <i>Sebacina</i> was the most-abundant OMF genera in the rhizosphere of <i>G. lutea</i> growing in the native forest soil. In contrast, <i>Thanatephorus</i> was the most abundant mycorrhizal taxa growing in the rhizosphere of orchids from the Coastal Cordillera. Besides, other OMF genera such as <i>Inocybe</i>, <i>Tomentella</i>, and <i>Mycena</i> were detected. The diversity of OMF in pelotons differed, being mainly related to <i>Ceratobasidium</i> sp. and <i>Tulasnella</i> sp. These results provide evidence of differences in OMF from pelotons and the rhizosphere soil in <i>G. lutea</i> growing in the Andes Cordillera and a selection of microbial communities in the rhizosphere of <i>C. collicensis</i> in the Coastal Cordillera. This raises questions about the efficiency of propagation strategies based only on mycorrhizal fungi obtained by culture-dependent methods, especially in orchids that depend on non-culturable taxa for seed germination and plantlet development. |
| first_indexed | 2024-03-09T04:14:55Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T04:14:55Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-3dc5a33a9f6f40c2bda5decf4c1d144d2023-12-03T13:54:58ZengMDPI AGJournal of Fungi2309-608X2022-07-018879410.3390/jof8080794Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central ChileHéctor Herrera0Tedy Sanhueza1Rafael Borges da Silva Valadares2Francisco Matus3Guillermo Pereira4Cristian Atala5María de la Luz Mora6Cesar Arriagada7Laboratorio de Biorremediación, Departamento de Ciencias Forestales, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Temuco 4780000, ChileLaboratorio de Biorremediación, Departamento de Ciencias Forestales, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Temuco 4780000, ChileInstituto Tecnologico Vale, Rua Boaventura da Silva 955, Belém 66055-090, PA, BrazilLaboratory of Conservation and Dynamics of Volcanic Soils, Department of Chemical Sciences and Natural Resources, Universidad de La Frontera, Temuco 4780000, ChileDepartamento de Ciencias y Tecnología Vegetal, Laboratorio Biotecnología de Hongos, Universidad de Concepción, Los Angeles 4440000, ChileInstituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaiso 2340000, ChileScientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 4780000, ChileLaboratorio de Biorremediación, Departamento de Ciencias Forestales, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Temuco 4780000, ChileThe diversity of orchid mycorrhizal fungi (OMF) and other beneficial root-associated fungi in temperate forests has scarcely been examined. This study aimed to analyze the diversity of mycorrhizal and rhizosphere-associated fungal communities in the terrestrial orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> growing in high-orchid-population-density areas in the piedmont of the Andes Cordillera with native forest (<i>Nothofagus-Araucaria</i>) and Coastal Cordillera with an exotic plantation (<i>Pinus-Eucalyptus</i>) in south-central Chile. We focused on rhizosphere-inhabiting and peloton-associated OMF in a native forest (Andes Cordillera) and a mixed forest (Coastal Cordillera). The native terrestrial orchids <i>G. lutea</i> and <i>C. collicensis</i> were localized, mycorrhizal root segments were taken to isolate peloton-associated OMF, and rhizosphere soil was taken to perform the metabarcoding approach. The results revealed that Basidiomycota and Ascomycota were the main rhizosphere-inhabiting fungal phyla, showing significant differences in the composition of fungal communities in both sites. <i>Sebacina</i> was the most-abundant OMF genera in the rhizosphere of <i>G. lutea</i> growing in the native forest soil. In contrast, <i>Thanatephorus</i> was the most abundant mycorrhizal taxa growing in the rhizosphere of orchids from the Coastal Cordillera. Besides, other OMF genera such as <i>Inocybe</i>, <i>Tomentella</i>, and <i>Mycena</i> were detected. The diversity of OMF in pelotons differed, being mainly related to <i>Ceratobasidium</i> sp. and <i>Tulasnella</i> sp. These results provide evidence of differences in OMF from pelotons and the rhizosphere soil in <i>G. lutea</i> growing in the Andes Cordillera and a selection of microbial communities in the rhizosphere of <i>C. collicensis</i> in the Coastal Cordillera. This raises questions about the efficiency of propagation strategies based only on mycorrhizal fungi obtained by culture-dependent methods, especially in orchids that depend on non-culturable taxa for seed germination and plantlet development.https://www.mdpi.com/2309-608X/8/8/794mycoheterotrophyOrchidaceaeorchid mycorrhizaesoil fungisymbiosis |
| spellingShingle | Héctor Herrera Tedy Sanhueza Rafael Borges da Silva Valadares Francisco Matus Guillermo Pereira Cristian Atala María de la Luz Mora Cesar Arriagada Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central Chile Journal of Fungi mycoheterotrophy Orchidaceae orchid mycorrhizae soil fungi symbiosis |
| title | Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central Chile |
| title_full | Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central Chile |
| title_fullStr | Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central Chile |
| title_full_unstemmed | Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central Chile |
| title_short | Diversity of Root-Associated Fungi of the Terrestrial Orchids <i>Gavilea lutea</i> and <i>Chloraea collicensis</i> in a Temperate Forest Soil of South-Central Chile |
| title_sort | diversity of root associated fungi of the terrestrial orchids i gavilea lutea i and i chloraea collicensis i in a temperate forest soil of south central chile |
| topic | mycoheterotrophy Orchidaceae orchid mycorrhizae soil fungi symbiosis |
| url | https://www.mdpi.com/2309-608X/8/8/794 |
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