A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection
The present study generated nectin1-mutant mice with single amino acid substitution and tested the anti-pseudorabies virus (PRV) ability of the mutant mice, with the aim to establish a model for PRV-resistant livestock. A phenylalanine to alanine transition at position 129 (F129A) of nectin1 was int...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/5/874 |
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| author | Xiaohui Yang Chuanzhao Yu Qiuyan Zhang Linjun Hong Ting Gu Enqin Zheng Zheng Xu Zicong Li Changxu Song Gengyuan Cai Zhenfang Wu Huaqiang Yang |
| author_facet | Xiaohui Yang Chuanzhao Yu Qiuyan Zhang Linjun Hong Ting Gu Enqin Zheng Zheng Xu Zicong Li Changxu Song Gengyuan Cai Zhenfang Wu Huaqiang Yang |
| author_sort | Xiaohui Yang |
| collection | DOAJ |
| description | The present study generated nectin1-mutant mice with single amino acid substitution and tested the anti-pseudorabies virus (PRV) ability of the mutant mice, with the aim to establish a model for PRV-resistant livestock. A phenylalanine to alanine transition at position 129 (F129A) of nectin1 was introduced into the mouse genome to generate nectin1 (F129A) mutant mice. The mutant mice were infected with a field-isolated highly virulent PRV strain by subcutaneous injection of virus. We found that the homozygous mutant mice had significantly alleviated disease manifestations and decreased death rate and viral loading in serum and tissue compared with heterozygous mutant and wild-type mice. In addition to disease resistance, the homozygous mutant mice showed a defect in eye development, indicating the side effect on animals by only one amino acid substitution in nectin1. Results demonstrate that gene modification in nectin1 is an effective approach to confer PRV resistance on animals, but the mutagenesis pattern requires further investigation to increase viral resistance without negative effect on animal development. |
| first_indexed | 2024-03-10T01:38:14Z |
| format | Article |
| id | doaj.art-3dc7947b6fb84f349f8baef064b82cdb |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:38:14Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3dc7947b6fb84f349f8baef064b82cdb2023-11-23T13:29:28ZengMDPI AGViruses1999-49152022-04-0114587410.3390/v14050874A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus InfectionXiaohui Yang0Chuanzhao Yu1Qiuyan Zhang2Linjun Hong3Ting Gu4Enqin Zheng5Zheng Xu6Zicong Li7Changxu Song8Gengyuan Cai9Zhenfang Wu10Huaqiang Yang11National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaThe present study generated nectin1-mutant mice with single amino acid substitution and tested the anti-pseudorabies virus (PRV) ability of the mutant mice, with the aim to establish a model for PRV-resistant livestock. A phenylalanine to alanine transition at position 129 (F129A) of nectin1 was introduced into the mouse genome to generate nectin1 (F129A) mutant mice. The mutant mice were infected with a field-isolated highly virulent PRV strain by subcutaneous injection of virus. We found that the homozygous mutant mice had significantly alleviated disease manifestations and decreased death rate and viral loading in serum and tissue compared with heterozygous mutant and wild-type mice. In addition to disease resistance, the homozygous mutant mice showed a defect in eye development, indicating the side effect on animals by only one amino acid substitution in nectin1. Results demonstrate that gene modification in nectin1 is an effective approach to confer PRV resistance on animals, but the mutagenesis pattern requires further investigation to increase viral resistance without negative effect on animal development.https://www.mdpi.com/1999-4915/14/5/874antiviral breedinggenetic modificationnectin1pigPRVdisease resistance |
| spellingShingle | Xiaohui Yang Chuanzhao Yu Qiuyan Zhang Linjun Hong Ting Gu Enqin Zheng Zheng Xu Zicong Li Changxu Song Gengyuan Cai Zhenfang Wu Huaqiang Yang A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection Viruses antiviral breeding genetic modification nectin1 pig PRV disease resistance |
| title | A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection |
| title_full | A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection |
| title_fullStr | A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection |
| title_full_unstemmed | A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection |
| title_short | A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection |
| title_sort | nectin1 mutant mouse model is resistant to pseudorabies virus infection |
| topic | antiviral breeding genetic modification nectin1 pig PRV disease resistance |
| url | https://www.mdpi.com/1999-4915/14/5/874 |
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