Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
The mitochondrial genome of moss Mielichhoferia elongata has been sequenced and assembled with Spades genome assembler. It consists of 100,342 base pairs and has practically the same gene set and order as in other known bryophyte chondriomes. The genome contains 66 genes including three rRNAs, 24 tR...
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| Natura: | Articolo |
| Lingua: | English |
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PeerJ Inc.
2018-02-01
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| Serie: | PeerJ |
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| Accesso online: | https://peerj.com/articles/4350.pdf |
| _version_ | 1827607699680919552 |
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| author | Denis V. Goruynov Svetlana V. Goryunova Oxana I. Kuznetsova Maria D. Logacheva Irina A. Milyutina Alina V. Fedorova Michael S. Ignatov Aleksey V. Troitsky |
| author_facet | Denis V. Goruynov Svetlana V. Goryunova Oxana I. Kuznetsova Maria D. Logacheva Irina A. Milyutina Alina V. Fedorova Michael S. Ignatov Aleksey V. Troitsky |
| author_sort | Denis V. Goruynov |
| collection | DOAJ |
| description | The mitochondrial genome of moss Mielichhoferia elongata has been sequenced and assembled with Spades genome assembler. It consists of 100,342 base pairs and has practically the same gene set and order as in other known bryophyte chondriomes. The genome contains 66 genes including three rRNAs, 24 tRNAs, and 40 conserved mitochondrial proteins genes. Unlike the majority of previously sequenced bryophyte mitogenomes, it lacks the functional nad7 gene. The phylogenetic reconstruction and scrutiny analysis of the primary structure of nad7 gene carried out in this study suggest its independent pseudogenization in different bryophyte lineages. Evaluation of the microsatellite (simple sequence repeat) content of the M. elongata mitochondrial genome indicates that it could be used as a tool in further studies as a phylogenetic marker. The strongly supported phylogenetic tree presented here, derived from 33 protein coding sequences of 40 bryophyte species, is consistent with other reconstructions based on a number of different data sets. |
| first_indexed | 2024-03-09T06:58:58Z |
| format | Article |
| id | doaj.art-3dc83a42a45142d09552e53631410c7f |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:58:58Z |
| publishDate | 2018-02-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-3dc83a42a45142d09552e53631410c7f2023-12-03T09:56:07ZengPeerJ Inc.PeerJ2167-83592018-02-016e435010.7717/peerj.4350Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality lossDenis V. Goruynov0Svetlana V. Goryunova1Oxana I. Kuznetsova2Maria D. Logacheva3Irina A. Milyutina4Alina V. Fedorova5Michael S. Ignatov6Aleksey V. Troitsky7Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaInstitute of General Genetics Russian Academy of Science, Moscow, RussiaTsitsin Main Botanical Garden Russian Academy of Science, Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaTsitsin Main Botanical Garden Russian Academy of Science, Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaThe mitochondrial genome of moss Mielichhoferia elongata has been sequenced and assembled with Spades genome assembler. It consists of 100,342 base pairs and has practically the same gene set and order as in other known bryophyte chondriomes. The genome contains 66 genes including three rRNAs, 24 tRNAs, and 40 conserved mitochondrial proteins genes. Unlike the majority of previously sequenced bryophyte mitogenomes, it lacks the functional nad7 gene. The phylogenetic reconstruction and scrutiny analysis of the primary structure of nad7 gene carried out in this study suggest its independent pseudogenization in different bryophyte lineages. Evaluation of the microsatellite (simple sequence repeat) content of the M. elongata mitochondrial genome indicates that it could be used as a tool in further studies as a phylogenetic marker. The strongly supported phylogenetic tree presented here, derived from 33 protein coding sequences of 40 bryophyte species, is consistent with other reconstructions based on a number of different data sets.https://peerj.com/articles/4350.pdfMitochondrial genomeBryophytaPhylogenetic tree“Copper moss” Mielichhoferia elongatanad7Simple sequence repeats |
| spellingShingle | Denis V. Goruynov Svetlana V. Goryunova Oxana I. Kuznetsova Maria D. Logacheva Irina A. Milyutina Alina V. Fedorova Michael S. Ignatov Aleksey V. Troitsky Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss PeerJ Mitochondrial genome Bryophyta Phylogenetic tree “Copper moss” Mielichhoferia elongata nad7 Simple sequence repeats |
| title | Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss |
| title_full | Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss |
| title_fullStr | Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss |
| title_full_unstemmed | Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss |
| title_short | Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss |
| title_sort | complete mitochondrial genome sequence of the copper moss mielichhoferia elongata reveals independent nad7 gene functionality loss |
| topic | Mitochondrial genome Bryophyta Phylogenetic tree “Copper moss” Mielichhoferia elongata nad7 Simple sequence repeats |
| url | https://peerj.com/articles/4350.pdf |
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