MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene
The differences of pork quality characteristics among different pig breeds mainly came from the differences in myofiber type compositions. Growing evidence indicated the key role of miRNAs in myofiber specification. In the present study, we found that miR-152 is more abundant in the slow-twitch myof...
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MDPI AG
2019-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/9/669 |
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| author | Yong Zhang Honglin Yan Pan Zhou Zhenzhen Zhang Jingbo Liu Hongfu Zhang |
| author_facet | Yong Zhang Honglin Yan Pan Zhou Zhenzhen Zhang Jingbo Liu Hongfu Zhang |
| author_sort | Yong Zhang |
| collection | DOAJ |
| description | The differences of pork quality characteristics among different pig breeds mainly came from the differences in myofiber type compositions. Growing evidence indicated the key role of miRNAs in myofiber specification. In the present study, we found that miR-152 is more abundant in the slow-twitch myofiber-enriched muscles. However, its role in myofiber type transformation and myogenesis is largely unknown. Overexpression of miR-152 in porcine myotubes promoted the formation of slow-twitch myofibers and myogenesis. While, inhibition of miR-152 expression showed the opposite effect to miR-152 mimics transfection. The luciferase reporter analysis confirmed that miR-152 straightly targets the 3′-untranslated region (3’-UTR) of uncoupling protein 3 (<i>UCP3</i>) to cause its post-transcriptional inhibition in the protein level. The knockdown of <i>UCP3</i> by siRNA showed the similar effect of miR-152 on myofiber type transition. Furthermore, the rescue experiment in the porcine myotube transfected with miR-152 mimics or/and <i>UCP3</i> overexpression plasmid with or without the 3’UTR revealed that <i>UCP3</i> mediates the action of miR-152 in slow-twitch myofiber formation. Taken together, our findings proposed a novel molecular mechanism through which miR-152 epigenetically regulates meat quality via promoting slow-twitch myofiber formation and skeletal myogenesis. |
| first_indexed | 2024-12-22T00:18:37Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-22T00:18:37Z |
| publishDate | 2019-09-01 |
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| spelling | doaj.art-3dcad6e8a30f4018b162bb75bd1581312022-12-21T18:45:14ZengMDPI AGAnimals2076-26152019-09-019966910.3390/ani9090669ani9090669MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 GeneYong Zhang0Honglin Yan1Pan Zhou2Zhenzhen Zhang3Jingbo Liu4Hongfu Zhang5School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaThe differences of pork quality characteristics among different pig breeds mainly came from the differences in myofiber type compositions. Growing evidence indicated the key role of miRNAs in myofiber specification. In the present study, we found that miR-152 is more abundant in the slow-twitch myofiber-enriched muscles. However, its role in myofiber type transformation and myogenesis is largely unknown. Overexpression of miR-152 in porcine myotubes promoted the formation of slow-twitch myofibers and myogenesis. While, inhibition of miR-152 expression showed the opposite effect to miR-152 mimics transfection. The luciferase reporter analysis confirmed that miR-152 straightly targets the 3′-untranslated region (3’-UTR) of uncoupling protein 3 (<i>UCP3</i>) to cause its post-transcriptional inhibition in the protein level. The knockdown of <i>UCP3</i> by siRNA showed the similar effect of miR-152 on myofiber type transition. Furthermore, the rescue experiment in the porcine myotube transfected with miR-152 mimics or/and <i>UCP3</i> overexpression plasmid with or without the 3’UTR revealed that <i>UCP3</i> mediates the action of miR-152 in slow-twitch myofiber formation. Taken together, our findings proposed a novel molecular mechanism through which miR-152 epigenetically regulates meat quality via promoting slow-twitch myofiber formation and skeletal myogenesis.https://www.mdpi.com/2076-2615/9/9/669miR-152UCP3myofiber specificationmyogenesismeat qualityporcine myoblasts |
| spellingShingle | Yong Zhang Honglin Yan Pan Zhou Zhenzhen Zhang Jingbo Liu Hongfu Zhang MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene Animals miR-152 UCP3 myofiber specification myogenesis meat quality porcine myoblasts |
| title | MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene |
| title_full | MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene |
| title_fullStr | MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene |
| title_full_unstemmed | MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene |
| title_short | MicroRNA-152 Promotes Slow-Twitch Myofiber Formation via Targeting Uncoupling Protein-3 Gene |
| title_sort | microrna 152 promotes slow twitch myofiber formation via targeting uncoupling protein 3 gene |
| topic | miR-152 UCP3 myofiber specification myogenesis meat quality porcine myoblasts |
| url | https://www.mdpi.com/2076-2615/9/9/669 |
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