miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis
Cognitive deficits strongly affect the quality of life of patients with multiple sclerosis (MS). However, no cognitive MS biomarkers are currently available. Extracellular vesicles (EVs) contain markers of parental cells and are able to pass from the brain into blood, representing a source of diseas...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/9/1551 |
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| author | Federica Scaroni Caterina Visconte Maria Serpente Maria Teresa Golia Martina Gabrielli Marijn Huiskamp Hanneke E. Hulst Tiziana Carandini Milena De Riz Anna Pietroboni Emanuela Rotondo Elio Scarpini Daniela Galimberti Charlotte E. Teunissen Maureen van Dam Brigit A. de Jong Chiara Fenoglio Claudia Verderio |
| author_facet | Federica Scaroni Caterina Visconte Maria Serpente Maria Teresa Golia Martina Gabrielli Marijn Huiskamp Hanneke E. Hulst Tiziana Carandini Milena De Riz Anna Pietroboni Emanuela Rotondo Elio Scarpini Daniela Galimberti Charlotte E. Teunissen Maureen van Dam Brigit A. de Jong Chiara Fenoglio Claudia Verderio |
| author_sort | Federica Scaroni |
| collection | DOAJ |
| description | Cognitive deficits strongly affect the quality of life of patients with multiple sclerosis (MS). However, no cognitive MS biomarkers are currently available. Extracellular vesicles (EVs) contain markers of parental cells and are able to pass from the brain into blood, representing a source of disease biomarkers. The aim of this study was to investigate whether small non-coding microRNAs (miRNAs) targeting synaptic genes and packaged in plasma EVs may reflect cognitive deficits in MS patients. Total EVs were precipitated by Exoquick from the plasma of twenty-six cognitively preserved (CP) and twenty-three cognitively impaired (CI) MS patients belonging to two independent cohorts. Myeloid EVs were extracted by affinity capture from total EVs using Isolectin B4 (IB4). Fourteen miRNAs targeting synaptic genes were selected and measured by RT-PCR in both total and myeloid EVs. Myeloid EVs from CI patients expressed higher levels of miR-150-5p and lower levels of let-7b-5p compared to CP patients. Stratification for progressive MS (PMS) and relapsing-remitting MS (RRMS) and correlation with clinical parameters suggested that these alterations might be attributable to cognitive deficits rather than disease progression. This study identifies miR-150-5p and let-7b-5p packaged in blood myeloid EVs as possible biomarkers for cognitive deficits in MS. |
| first_indexed | 2024-03-10T04:15:49Z |
| format | Article |
| id | doaj.art-3dcd09eb642445cba9cfcaae9efec2de |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:15:49Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3dcd09eb642445cba9cfcaae9efec2de2023-11-23T08:00:56ZengMDPI AGCells2073-44092022-05-01119155110.3390/cells11091551miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple SclerosisFederica Scaroni0Caterina Visconte1Maria Serpente2Maria Teresa Golia3Martina Gabrielli4Marijn Huiskamp5Hanneke E. Hulst6Tiziana Carandini7Milena De Riz8Anna Pietroboni9Emanuela Rotondo10Elio Scarpini11Daniela Galimberti12Charlotte E. Teunissen13Maureen van Dam14Brigit A. de Jong15Chiara Fenoglio16Claudia Verderio17Institute of Neuroscience, CNR, Via Follereau 3, 20854 Vedano al Lambro, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via F. Sforza 35, 20122 Milan, ItalyCentro Dino Ferrari, University of Milan, 20122 Milan, ItalyInstitute of Neuroscience, CNR, Via Follereau 3, 20854 Vedano al Lambro, ItalyInstitute of Neuroscience, CNR, Via Follereau 3, 20854 Vedano al Lambro, ItalyMS Center Amsterdam, Amsterdam Neuroscience, Department of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit Amsterdam UMC, De Boelelaan 1117, 1081 Amsterdam, The NetherlandsHealth-, Medical- and Neuropsychology Unit, Institute of Psychology, Leiden University, 2300 Leiden, The NetherlandsCentro Dino Ferrari, University of Milan, 20122 Milan, ItalyCentro Dino Ferrari, University of Milan, 20122 Milan, ItalyCentro Dino Ferrari, University of Milan, 20122 Milan, ItalyCentro Dino Ferrari, University of Milan, 20122 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via F. Sforza 35, 20122 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Via F. Sforza 35, 20122 Milan, ItalyMS Center Amsterdam, Amsterdam Neuroscience, Department of Neurology, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 Amsterdam, The NetherlandsMS Center Amsterdam, Amsterdam Neuroscience, Department of Anatomy and Neurosciences, Amsterdam UMC, Vrije Universiteit Amsterdam UMC, De Boelelaan 1117, 1081 Amsterdam, The NetherlandsMS Center Amsterdam, Amsterdam Neuroscience, Department of Neurology, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 Amsterdam, The NetherlandsMS Center Amsterdam, Amsterdam Neuroscience, Department of Neurology, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, 1081 Amsterdam, The NetherlandsInstitute of Neuroscience, CNR, Via Follereau 3, 20854 Vedano al Lambro, ItalyCognitive deficits strongly affect the quality of life of patients with multiple sclerosis (MS). However, no cognitive MS biomarkers are currently available. Extracellular vesicles (EVs) contain markers of parental cells and are able to pass from the brain into blood, representing a source of disease biomarkers. The aim of this study was to investigate whether small non-coding microRNAs (miRNAs) targeting synaptic genes and packaged in plasma EVs may reflect cognitive deficits in MS patients. Total EVs were precipitated by Exoquick from the plasma of twenty-six cognitively preserved (CP) and twenty-three cognitively impaired (CI) MS patients belonging to two independent cohorts. Myeloid EVs were extracted by affinity capture from total EVs using Isolectin B4 (IB4). Fourteen miRNAs targeting synaptic genes were selected and measured by RT-PCR in both total and myeloid EVs. Myeloid EVs from CI patients expressed higher levels of miR-150-5p and lower levels of let-7b-5p compared to CP patients. Stratification for progressive MS (PMS) and relapsing-remitting MS (RRMS) and correlation with clinical parameters suggested that these alterations might be attributable to cognitive deficits rather than disease progression. This study identifies miR-150-5p and let-7b-5p packaged in blood myeloid EVs as possible biomarkers for cognitive deficits in MS.https://www.mdpi.com/2073-4409/11/9/1551multiple sclerosisplasma extracellular vesiclesmyeloid extracellular vesiclesmicroRNAscognitive deficitsbiomarkers |
| spellingShingle | Federica Scaroni Caterina Visconte Maria Serpente Maria Teresa Golia Martina Gabrielli Marijn Huiskamp Hanneke E. Hulst Tiziana Carandini Milena De Riz Anna Pietroboni Emanuela Rotondo Elio Scarpini Daniela Galimberti Charlotte E. Teunissen Maureen van Dam Brigit A. de Jong Chiara Fenoglio Claudia Verderio miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis Cells multiple sclerosis plasma extracellular vesicles myeloid extracellular vesicles microRNAs cognitive deficits biomarkers |
| title | miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis |
| title_full | miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis |
| title_fullStr | miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis |
| title_full_unstemmed | miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis |
| title_short | miR-150-5p and let-7b-5p in Blood Myeloid Extracellular Vesicles Track Cognitive Symptoms in Patients with Multiple Sclerosis |
| title_sort | mir 150 5p and let 7b 5p in blood myeloid extracellular vesicles track cognitive symptoms in patients with multiple sclerosis |
| topic | multiple sclerosis plasma extracellular vesicles myeloid extracellular vesicles microRNAs cognitive deficits biomarkers |
| url | https://www.mdpi.com/2073-4409/11/9/1551 |
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