Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i>
A new method was proposed to produce alginate bio-films containing <i>Pichia membranifaciens</i> and <i>Wickerhamomyces anomalus</i> killer yeast to control the post-harvest fungal decay in organic apples caused by <i>Botrytis cinerea</i> and <i>Penicillium...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/13/1868 |
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| author | Urszula Błaszczyk Sylwia Wyrzykowska Maciej Gąstoł |
| author_facet | Urszula Błaszczyk Sylwia Wyrzykowska Maciej Gąstoł |
| author_sort | Urszula Błaszczyk |
| collection | DOAJ |
| description | A new method was proposed to produce alginate bio-films containing <i>Pichia membranifaciens</i> and <i>Wickerhamomyces anomalus</i> killer yeast to control the post-harvest fungal decay in organic apples caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i>. Coatings with <i>W. anomalus</i> killer yeast effectively controlled the growth of <i>P. italicum</i> during storage at 22 °C. <i>W. anomalus</i> killer yeast incorporated in alginate reduced the <i>P. italicum</i> incidence from 90% (control) to 35% after 14 days of storage at 22 °C. Alginate biofilms with <i>W. anomalus</i> or <i>P. membranifaciens</i> also limited the incidence of the fungal decay of apples inoculated with <i>B. cinerea</i> compared with the control fruits, although the antagonistic capability against <i>B. cinerea</i> was lower than against <i>P. italicum</i>. The survival of <i>W. anomalus</i> cells in alginate coating was higher than <i>P. membranifaciens</i>. The incorporation of killer yeasts into alginate had no significant effect on the mechanical properties (tensile strength, percent elongation at break) of alginate coating, however, they increased the thickness of the biofilm. The bioactive coating reduced the fruit weight loss and had no significant effects on the fruit firmness during storage at 2 °C. As organic apples, produced without any synthetic fungicides, are especially prone to fungal decay during storage, the proposed alginate biofilms containing killer yeast seem to be a very promising solution by offering non-chemical, biological control of post-harvest pathogens. |
| first_indexed | 2024-03-09T21:54:38Z |
| format | Article |
| id | doaj.art-3dcefe38948146e393c30a0ef2beca18 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T21:54:38Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-3dcefe38948146e393c30a0ef2beca182023-11-23T20:00:29ZengMDPI AGFoods2304-81582022-06-011113186810.3390/foods11131868Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i>Urszula Błaszczyk0Sylwia Wyrzykowska1Maciej Gąstoł2Department of Fermentation Technology and Microbiology, Faculty of Food Technology, University of Agriculture in Krakow, Aleja Mickiewicza 21, 31-120 Krakow, PolandDepartment of Fermentation Technology and Microbiology, Faculty of Food Technology, University of Agriculture in Krakow, Aleja Mickiewicza 21, 31-120 Krakow, PolandDepartment of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Aleja Mickiewicza 21, 31-120 Krakow, PolandA new method was proposed to produce alginate bio-films containing <i>Pichia membranifaciens</i> and <i>Wickerhamomyces anomalus</i> killer yeast to control the post-harvest fungal decay in organic apples caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i>. Coatings with <i>W. anomalus</i> killer yeast effectively controlled the growth of <i>P. italicum</i> during storage at 22 °C. <i>W. anomalus</i> killer yeast incorporated in alginate reduced the <i>P. italicum</i> incidence from 90% (control) to 35% after 14 days of storage at 22 °C. Alginate biofilms with <i>W. anomalus</i> or <i>P. membranifaciens</i> also limited the incidence of the fungal decay of apples inoculated with <i>B. cinerea</i> compared with the control fruits, although the antagonistic capability against <i>B. cinerea</i> was lower than against <i>P. italicum</i>. The survival of <i>W. anomalus</i> cells in alginate coating was higher than <i>P. membranifaciens</i>. The incorporation of killer yeasts into alginate had no significant effect on the mechanical properties (tensile strength, percent elongation at break) of alginate coating, however, they increased the thickness of the biofilm. The bioactive coating reduced the fruit weight loss and had no significant effects on the fruit firmness during storage at 2 °C. As organic apples, produced without any synthetic fungicides, are especially prone to fungal decay during storage, the proposed alginate biofilms containing killer yeast seem to be a very promising solution by offering non-chemical, biological control of post-harvest pathogens.https://www.mdpi.com/2304-8158/11/13/1868biological controlkiller yeast<i>Botrytis cinerea</i><i>Penicillium italicum</i>green practices and processes |
| spellingShingle | Urszula Błaszczyk Sylwia Wyrzykowska Maciej Gąstoł Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i> Foods biological control killer yeast <i>Botrytis cinerea</i> <i>Penicillium italicum</i> green practices and processes |
| title | Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i> |
| title_full | Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i> |
| title_fullStr | Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i> |
| title_full_unstemmed | Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i> |
| title_short | Application of Bioactive Coatings with Killer Yeasts to Control Post-Harvest Apple Decay Caused by <i>Botrytis cinerea</i> and <i>Penicillium italicum</i> |
| title_sort | application of bioactive coatings with killer yeasts to control post harvest apple decay caused by i botrytis cinerea i and i penicillium italicum i |
| topic | biological control killer yeast <i>Botrytis cinerea</i> <i>Penicillium italicum</i> green practices and processes |
| url | https://www.mdpi.com/2304-8158/11/13/1868 |
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