GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i>
Phytochemical analyses as well as antimicrobial and antioxidant activities of the extracts of C. sumatrensis aerial parts were investigated in this study. METHODS: The aerial parts of C. sumatrensis were air dried, weighed and exhaustively extracted with hexane, ethyl acetate and methanol success...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2016-05-01
|
| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/135147 |
| _version_ | 1797197451115888640 |
|---|---|
| author | Olapeju O Aiyelaagbe Stephen O Oguntoye Abdulmumeen A Hamid Adebola M Ogundare David B Ojo Ajibola Ajao Nurudeen O Owolabi |
| author_facet | Olapeju O Aiyelaagbe Stephen O Oguntoye Abdulmumeen A Hamid Adebola M Ogundare David B Ojo Ajibola Ajao Nurudeen O Owolabi |
| author_sort | Olapeju O Aiyelaagbe |
| collection | DOAJ |
| description |
Phytochemical analyses as well as antimicrobial and antioxidant activities of the extracts of C. sumatrensis aerial parts were investigated in this study.
METHODS: The aerial parts of C. sumatrensis were air dried, weighed and exhaustively extracted with hexane, ethyl acetate and methanol successively. The crude extracts were screened for metabolites. These extracts of the plant were evaluated for antimicrobial and antioxidant activities using agar diffusion and DPPH method respectively. The extracts were also analysed using Gas chromatography – Mass spectrometry, and the chromatogram coupled with mass spectra of the compounds were matched with a standard library.
RESULTS: Preliminary phytochemical investigation of rude n-hexane, ethyl acetate and methanol extracts of the aerial parts of Conyza sumatrensis revealed the presence of anthraquinones, flavonoids, terpenoids, phenolics, tannin, glycosides and carbohydrate. All the crude extracts gave a clear zone of inhibition against the growth of the test bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomona aeruginosa, Salmonella typhi, Klebsiellae pneumonae) at moderate to high concentrations, as well as test fungi (Candida albicans, Aspergillus niger, penicillium notatum and Rhizopus stolonifer) at high concentration. Methanolic extract exhibited significant radical scavenging property with IC50 of 17.08 μg/mL while n-hexane and ethyl acetate extracts showed no significant antioxidant activity. GC-MS of N-hexane extract showed a total number of eleven chemical constituents with α-Farnesene and spathulenol being the most abundance compounds constituting 20.27 and 22.28% of the extract respectively. Ethyl acetate extract revealed thirteen compounds with two most abundant compounds, cis-β-farnesene (16.64 %) and cis-pinane (21.09 %). While methanolic extract affords seventeen compounds with Ephytol being the most abundant compound (19.36 %). © JASEM
Keywords: Antimicrobial activity, Antioxidant, GC-MS analysis, Phytochemicals, Conyza sumatrensis
|
| first_indexed | 2024-04-24T06:44:10Z |
| format | Article |
| id | doaj.art-3dd02086ddc74031b396315068b77fe1 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T06:44:10Z |
| publishDate | 2016-05-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-3dd02086ddc74031b396315068b77fe12024-04-22T19:47:04ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992016-05-01201GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i>Olapeju O AiyelaagbeStephen O OguntoyeAbdulmumeen A HamidAdebola M OgundareDavid B OjoAjibola AjaoNurudeen O Owolabi Phytochemical analyses as well as antimicrobial and antioxidant activities of the extracts of C. sumatrensis aerial parts were investigated in this study. METHODS: The aerial parts of C. sumatrensis were air dried, weighed and exhaustively extracted with hexane, ethyl acetate and methanol successively. The crude extracts were screened for metabolites. These extracts of the plant were evaluated for antimicrobial and antioxidant activities using agar diffusion and DPPH method respectively. The extracts were also analysed using Gas chromatography – Mass spectrometry, and the chromatogram coupled with mass spectra of the compounds were matched with a standard library. RESULTS: Preliminary phytochemical investigation of rude n-hexane, ethyl acetate and methanol extracts of the aerial parts of Conyza sumatrensis revealed the presence of anthraquinones, flavonoids, terpenoids, phenolics, tannin, glycosides and carbohydrate. All the crude extracts gave a clear zone of inhibition against the growth of the test bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomona aeruginosa, Salmonella typhi, Klebsiellae pneumonae) at moderate to high concentrations, as well as test fungi (Candida albicans, Aspergillus niger, penicillium notatum and Rhizopus stolonifer) at high concentration. Methanolic extract exhibited significant radical scavenging property with IC50 of 17.08 μg/mL while n-hexane and ethyl acetate extracts showed no significant antioxidant activity. GC-MS of N-hexane extract showed a total number of eleven chemical constituents with α-Farnesene and spathulenol being the most abundance compounds constituting 20.27 and 22.28% of the extract respectively. Ethyl acetate extract revealed thirteen compounds with two most abundant compounds, cis-β-farnesene (16.64 %) and cis-pinane (21.09 %). While methanolic extract affords seventeen compounds with Ephytol being the most abundant compound (19.36 %). © JASEM Keywords: Antimicrobial activity, Antioxidant, GC-MS analysis, Phytochemicals, Conyza sumatrensis https://www.ajol.info/index.php/jasem/article/view/135147Antimicrobial activityAntioxidantGC-MS analysisPhytochemicalsConyza sumatrensis |
| spellingShingle | Olapeju O Aiyelaagbe Stephen O Oguntoye Abdulmumeen A Hamid Adebola M Ogundare David B Ojo Ajibola Ajao Nurudeen O Owolabi GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i> Journal of Applied Sciences and Environmental Management Antimicrobial activity Antioxidant GC-MS analysis Phytochemicals Conyza sumatrensis |
| title | GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i> |
| title_full | GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i> |
| title_fullStr | GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i> |
| title_full_unstemmed | GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i> |
| title_short | GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of <i>Conyza sumatrensis</i> |
| title_sort | gc ms analysis antimicrobial and antioxidant activities of extracts of the aerial parts of i conyza sumatrensis i |
| topic | Antimicrobial activity Antioxidant GC-MS analysis Phytochemicals Conyza sumatrensis |
| url | https://www.ajol.info/index.php/jasem/article/view/135147 |
| work_keys_str_mv | AT olapejuoaiyelaagbe gcmsanalysisantimicrobialandantioxidantactivitiesofextractsoftheaerialpartsoficonyzasumatrensisi AT stephenooguntoye gcmsanalysisantimicrobialandantioxidantactivitiesofextractsoftheaerialpartsoficonyzasumatrensisi AT abdulmumeenahamid gcmsanalysisantimicrobialandantioxidantactivitiesofextractsoftheaerialpartsoficonyzasumatrensisi AT adebolamogundare gcmsanalysisantimicrobialandantioxidantactivitiesofextractsoftheaerialpartsoficonyzasumatrensisi AT davidbojo gcmsanalysisantimicrobialandantioxidantactivitiesofextractsoftheaerialpartsoficonyzasumatrensisi AT ajibolaajao gcmsanalysisantimicrobialandantioxidantactivitiesofextractsoftheaerialpartsoficonyzasumatrensisi AT nurudeenoowolabi gcmsanalysisantimicrobialandantioxidantactivitiesofextractsoftheaerialpartsoficonyzasumatrensisi |