Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile
<p>Abstract</p> <p>Background</p> <p><it>Staphylococcus epidermidis </it>has emerged as one of the most important nosocomial pathogens, mainly because of its ability to colonize implanted biomaterials by forming a biofilm. Extensive studies are focused on th...
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| Format: | Article |
| Language: | English |
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BMC
2010-11-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/287 |
| _version_ | 1819183021306150912 |
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| author | Yu Fangyou Hu Jian Wu Yang Lou Qiang Zhu Tao Qu Di |
| author_facet | Yu Fangyou Hu Jian Wu Yang Lou Qiang Zhu Tao Qu Di |
| author_sort | Yu Fangyou |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Staphylococcus epidermidis </it>has emerged as one of the most important nosocomial pathogens, mainly because of its ability to colonize implanted biomaterials by forming a biofilm. Extensive studies are focused on the molecular mechanisms involved in biofilm formation. The LytSR two-component regulatory system regulates autolysis and biofilm formation in <it>Staphylococcus aureus</it>. However, the role of LytSR played in <it>S. epidermidis </it>remained unknown.</p> <p>Results</p> <p>In the present study, we demonstrated that <it>lytSR </it>knock-out in <it>S. epidermidis </it>did not alter susceptibility to Triton X-100 induced autolysis. Quantitative murein hydrolase assay indicated that disruption of <it>lytSR </it>in <it>S. epidermidis </it>resulted in decreased activities of extracellular murein hydrolases, although zymogram showed no apparent differences in murein hydrolase patterns between <it>S. epidermidis </it>strain 1457 and its <it>lytSR </it>mutant. Compared to the wild-type counterpart, 1457<it>ΔlytSR</it> produced slightly more biofilm, with significantly decreased dead cells inside. Microarray analysis showed that <it>lytSR </it>mutation affected the transcription of 164 genes (123 genes were upregulated and 41 genes were downregulated). Specifically, genes encoding proteins responsible for protein synthesis, energy metabolism were downregulated, while genes involved in amino acid and nucleotide biosynthesis, amino acid transporters were upregulated. Impaired ability to utilize pyruvate and reduced activity of arginine deiminase was observed in 1457<it>ΔlytSR</it>, which is consistent with the microarray data.</p> <p>Conclusions</p> <p>The preliminary results suggest that in <it>S. epidermidis </it>LytSR two-component system regulates extracellular murein hydrolase activity, bacterial cell death and pyruvate utilization. Based on the microarray data, it appears that <it>lytSR </it>inactivation induces a stringent response. In addition, LytSR may indirectly enhance biofilm formation by altering the metabolic status of the bacteria.</p> |
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| issn | 1471-2180 |
| language | English |
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| publishDate | 2010-11-01 |
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| spelling | doaj.art-3dd3a5392d754a0292671da8c0fc67a52022-12-21T18:09:50ZengBMCBMC Microbiology1471-21802010-11-0110128710.1186/1471-2180-10-287Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profileYu FangyouHu JianWu YangLou QiangZhu TaoQu Di<p>Abstract</p> <p>Background</p> <p><it>Staphylococcus epidermidis </it>has emerged as one of the most important nosocomial pathogens, mainly because of its ability to colonize implanted biomaterials by forming a biofilm. Extensive studies are focused on the molecular mechanisms involved in biofilm formation. The LytSR two-component regulatory system regulates autolysis and biofilm formation in <it>Staphylococcus aureus</it>. However, the role of LytSR played in <it>S. epidermidis </it>remained unknown.</p> <p>Results</p> <p>In the present study, we demonstrated that <it>lytSR </it>knock-out in <it>S. epidermidis </it>did not alter susceptibility to Triton X-100 induced autolysis. Quantitative murein hydrolase assay indicated that disruption of <it>lytSR </it>in <it>S. epidermidis </it>resulted in decreased activities of extracellular murein hydrolases, although zymogram showed no apparent differences in murein hydrolase patterns between <it>S. epidermidis </it>strain 1457 and its <it>lytSR </it>mutant. Compared to the wild-type counterpart, 1457<it>ΔlytSR</it> produced slightly more biofilm, with significantly decreased dead cells inside. Microarray analysis showed that <it>lytSR </it>mutation affected the transcription of 164 genes (123 genes were upregulated and 41 genes were downregulated). Specifically, genes encoding proteins responsible for protein synthesis, energy metabolism were downregulated, while genes involved in amino acid and nucleotide biosynthesis, amino acid transporters were upregulated. Impaired ability to utilize pyruvate and reduced activity of arginine deiminase was observed in 1457<it>ΔlytSR</it>, which is consistent with the microarray data.</p> <p>Conclusions</p> <p>The preliminary results suggest that in <it>S. epidermidis </it>LytSR two-component system regulates extracellular murein hydrolase activity, bacterial cell death and pyruvate utilization. Based on the microarray data, it appears that <it>lytSR </it>inactivation induces a stringent response. In addition, LytSR may indirectly enhance biofilm formation by altering the metabolic status of the bacteria.</p>http://www.biomedcentral.com/1471-2180/10/287 |
| spellingShingle | Yu Fangyou Hu Jian Wu Yang Lou Qiang Zhu Tao Qu Di Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile BMC Microbiology |
| title | Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile |
| title_full | Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile |
| title_fullStr | Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile |
| title_full_unstemmed | Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile |
| title_short | Impact of the <it>Staphylococcus epidermidis </it>LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile |
| title_sort | impact of the it staphylococcus epidermidis it lytsr two component regulatory system on murein hydrolase activity pyruvate utilization and global transcriptional profile |
| url | http://www.biomedcentral.com/1471-2180/10/287 |
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