Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis
ABSTRACTBackground Mfa1 fimbriae of the periodontal pathogen Porphyromonas gingivalis are responsible for biofilm formation and comprise five proteins: Mfa1–5. Two major genotypes, mfa170 and mfa153, encode major fimbrillin. The mfa170 genotype is further divided into the mfa170A and mfa170B subtype...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2023.2215551 |
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| author | Miyuna Fujimoto Yoshikazu Naiki Kotaro Sakae Tomohiko Iwase Naoyoshi Miwa Keiji Nagano Hiroyuki Nawa Yoshiaki Hasegawa |
| author_facet | Miyuna Fujimoto Yoshikazu Naiki Kotaro Sakae Tomohiko Iwase Naoyoshi Miwa Keiji Nagano Hiroyuki Nawa Yoshiaki Hasegawa |
| author_sort | Miyuna Fujimoto |
| collection | DOAJ |
| description | ABSTRACTBackground Mfa1 fimbriae of the periodontal pathogen Porphyromonas gingivalis are responsible for biofilm formation and comprise five proteins: Mfa1–5. Two major genotypes, mfa170 and mfa153, encode major fimbrillin. The mfa170 genotype is further divided into the mfa170A and mfa170B subtypes. The properties of the novel mfa170B remain unclear.Methods Fimbriae were purified from P. gingivalis strains JI-1 (mfa170A), 1439 (mfa170B), and Ando (mfa153), and their components and their structures were analyzed. Protein expression and variability in the antigenic specificity of fimbrillins were compared using Coomassie staining and western blotting using polyclonal antibodies against Mfa170A, Mfa170B, and Mfa153 proteins. Cell surface expression levels of fimbriae were analyzed by filtration enzyme-linked immunosorbent assays.Results The composition and structure of the purified Mfa1 fimbriae of 1439 was similar to that of JI-1. However, each Mfa1 protein of differential subtype/genotype was specifically detected by western blotting. Mfa170B fimbriae were expressed in several strains such as 1439, JKG9, B42, 1436, and Kyudai-3. Differential protein expression and antigenic heterogeneities were detected in Mfa2–5 between strains.Conclusion Mfa1 fimbriae from the mfa170A and mfa170B genotypes indicated an antigenic differencesuggesting the mfa170B, is to be utilized for the novel classification of P. gingivalis. |
| first_indexed | 2024-03-09T14:33:34Z |
| format | Article |
| id | doaj.art-3dd62b6398324067a69a431dcd24901c |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-03-09T14:33:34Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-3dd62b6398324067a69a431dcd24901c2023-11-27T17:25:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972023-12-0115110.1080/20002297.2023.2215551Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalisMiyuna Fujimoto0Yoshikazu Naiki1Kotaro Sakae2Tomohiko Iwase3Naoyoshi Miwa4Keiji Nagano5Hiroyuki Nawa6Yoshiaki Hasegawa7Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, JapanDepartment of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, JapanDepartment of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, JapanDepartment of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, JapanDepartment of Pediatric Dentistry, School of Dentistry, Aichi Gakuin University, Nagoya, JapanDivision of Microbiology, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, JapanDepartment of Pediatric Dentistry, School of Dentistry, Aichi Gakuin University, Nagoya, JapanDepartment of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, JapanABSTRACTBackground Mfa1 fimbriae of the periodontal pathogen Porphyromonas gingivalis are responsible for biofilm formation and comprise five proteins: Mfa1–5. Two major genotypes, mfa170 and mfa153, encode major fimbrillin. The mfa170 genotype is further divided into the mfa170A and mfa170B subtypes. The properties of the novel mfa170B remain unclear.Methods Fimbriae were purified from P. gingivalis strains JI-1 (mfa170A), 1439 (mfa170B), and Ando (mfa153), and their components and their structures were analyzed. Protein expression and variability in the antigenic specificity of fimbrillins were compared using Coomassie staining and western blotting using polyclonal antibodies against Mfa170A, Mfa170B, and Mfa153 proteins. Cell surface expression levels of fimbriae were analyzed by filtration enzyme-linked immunosorbent assays.Results The composition and structure of the purified Mfa1 fimbriae of 1439 was similar to that of JI-1. However, each Mfa1 protein of differential subtype/genotype was specifically detected by western blotting. Mfa170B fimbriae were expressed in several strains such as 1439, JKG9, B42, 1436, and Kyudai-3. Differential protein expression and antigenic heterogeneities were detected in Mfa2–5 between strains.Conclusion Mfa1 fimbriae from the mfa170A and mfa170B genotypes indicated an antigenic differencesuggesting the mfa170B, is to be utilized for the novel classification of P. gingivalis.https://www.tandfonline.com/doi/10.1080/20002297.2023.2215551Porphyromonas gingivalisMfa1fimbriaeperiodontitisgenotype |
| spellingShingle | Miyuna Fujimoto Yoshikazu Naiki Kotaro Sakae Tomohiko Iwase Naoyoshi Miwa Keiji Nagano Hiroyuki Nawa Yoshiaki Hasegawa Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis Journal of Oral Microbiology Porphyromonas gingivalis Mfa1 fimbriae periodontitis genotype |
| title | Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis |
| title_full | Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis |
| title_fullStr | Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis |
| title_full_unstemmed | Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis |
| title_short | Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis |
| title_sort | structural and antigenic characterization of a novel genotype of mfa1 fimbriae in porphyromonas gingivalis |
| topic | Porphyromonas gingivalis Mfa1 fimbriae periodontitis genotype |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2023.2215551 |
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