Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress
Sorghum has recently attracted much attention for its tolerance in high salt environment. However, the effect and regulatory mechanism of the gibberellic acid (GA3)-mediated alleviation of salt stress in sorghum remains unclear. Herein, we reported that a GA3 concentration of 50 mg/L is optimal for...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1071657/full |
| _version_ | 1811210618071416832 |
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| author | Yanqing Wu Yanqing Wu Jiao Liu Jiao Liu Guisheng Zhou Guisheng Zhou |
| author_facet | Yanqing Wu Yanqing Wu Jiao Liu Jiao Liu Guisheng Zhou Guisheng Zhou |
| author_sort | Yanqing Wu |
| collection | DOAJ |
| description | Sorghum has recently attracted much attention for its tolerance in high salt environment. However, the effect and regulatory mechanism of the gibberellic acid (GA3)-mediated alleviation of salt stress in sorghum remains unclear. Herein, we reported that a GA3 concentration of 50 mg/L is optimal for sorghum (“Jitian 3”) development under salt stress. We conducted a whole-transcriptome analysis between GA3-treated and control sorghum leaves under salt stress, and we identified 1002 differentially expressed (DE)-messenger RNAs (mRNAs), 81 DE-long non-coding RNAs (lncRNAs), 7 DE-circular RNAs (circRNAs), and 26 DE-microRNA (miRNAs) in sorghum following GA3 treatment. We also identified a majority of DE-mRNAs and non-coding RNAs (ncRNAs) targets that serve essential roles in phenylpropanoid biosynthesis and plant hormone networks. In addition, we generated a competitive endogenous RNA (ceRNA)-miRNA-target gene network, and 3 circRNAs (circRNA_2746, circRNA_6515, circRNA_5622), 4 lncRNAs (XR_002450182.1, XR_002452422.1, XR_002448510.1, XR_002448296.1) and 4 genes (LOC8056546, LOC8062245, LOC8061469, LOC8071960) probably act as valuable candidates for the regulation of the GA3-mediated alleviation of salt stress in sorghum. Our findings uncovered potential mRNA and non-coding RNAs that contribute to GA3 regulation, thus offering a basis for the future investigation of underlying mechanisms of salt stress in sorghum. |
| first_indexed | 2024-04-12T04:57:47Z |
| format | Article |
| id | doaj.art-3dd7eeacac334879a3fa335847764cb6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T04:57:47Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3dd7eeacac334879a3fa335847764cb62022-12-22T03:47:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10716571071657Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stressYanqing Wu0Yanqing Wu1Jiao Liu2Jiao Liu3Guisheng Zhou4Guisheng Zhou5Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, Jiangsu, ChinaSorghum has recently attracted much attention for its tolerance in high salt environment. However, the effect and regulatory mechanism of the gibberellic acid (GA3)-mediated alleviation of salt stress in sorghum remains unclear. Herein, we reported that a GA3 concentration of 50 mg/L is optimal for sorghum (“Jitian 3”) development under salt stress. We conducted a whole-transcriptome analysis between GA3-treated and control sorghum leaves under salt stress, and we identified 1002 differentially expressed (DE)-messenger RNAs (mRNAs), 81 DE-long non-coding RNAs (lncRNAs), 7 DE-circular RNAs (circRNAs), and 26 DE-microRNA (miRNAs) in sorghum following GA3 treatment. We also identified a majority of DE-mRNAs and non-coding RNAs (ncRNAs) targets that serve essential roles in phenylpropanoid biosynthesis and plant hormone networks. In addition, we generated a competitive endogenous RNA (ceRNA)-miRNA-target gene network, and 3 circRNAs (circRNA_2746, circRNA_6515, circRNA_5622), 4 lncRNAs (XR_002450182.1, XR_002452422.1, XR_002448510.1, XR_002448296.1) and 4 genes (LOC8056546, LOC8062245, LOC8061469, LOC8071960) probably act as valuable candidates for the regulation of the GA3-mediated alleviation of salt stress in sorghum. Our findings uncovered potential mRNA and non-coding RNAs that contribute to GA3 regulation, thus offering a basis for the future investigation of underlying mechanisms of salt stress in sorghum.https://www.frontiersin.org/articles/10.3389/fpls.2022.1071657/fullsorghumgibberellic acid (GA3)whole-transcriptomesalt stressceRNA |
| spellingShingle | Yanqing Wu Yanqing Wu Jiao Liu Jiao Liu Guisheng Zhou Guisheng Zhou Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress Frontiers in Plant Science sorghum gibberellic acid (GA3) whole-transcriptome salt stress ceRNA |
| title | Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress |
| title_full | Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress |
| title_fullStr | Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress |
| title_full_unstemmed | Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress |
| title_short | Whole-transcriptome analyses of Sorghum leaves identify key mRNAs and ncRNAs associated with GA3-mediated alleviation of salt stress |
| title_sort | whole transcriptome analyses of sorghum leaves identify key mrnas and ncrnas associated with ga3 mediated alleviation of salt stress |
| topic | sorghum gibberellic acid (GA3) whole-transcriptome salt stress ceRNA |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1071657/full |
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