Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of bone
The aim of the present study was to further improve an in vitro 3D osteoblast (OB) – osteoclast (OC) co-culture model of bone by tuning it towards states of formation, resorption, and equilibrium for their future applications in fundamental research, drug development and personalized medicine. This...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
|
| Series: | Bone Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352187222004806 |
| _version_ | 1797798061509967872 |
|---|---|
| author | Stefan J.A. Remmers Freek C. van der Heijden Bregje W.M. de Wildt Keita Ito Sandra Hofmann |
| author_facet | Stefan J.A. Remmers Freek C. van der Heijden Bregje W.M. de Wildt Keita Ito Sandra Hofmann |
| author_sort | Stefan J.A. Remmers |
| collection | DOAJ |
| description | The aim of the present study was to further improve an in vitro 3D osteoblast (OB) – osteoclast (OC) co-culture model of bone by tuning it towards states of formation, resorption, and equilibrium for their future applications in fundamental research, drug development and personalized medicine. This was achieved by varying culture medium composition and monocyte seeding density, the two external parameters that affect cell behavior the most. Monocytes were seeded at two seeding densities onto 3D silk-fibroin constructs pre-mineralized by MSC-derived OBs and were co-cultured in one of three different media (OC stimulating, Neutral and OB stimulating medium) for three weeks. Histology showed mineralized matrix after co-culture and OC markers in the OC medium group. Scanning Electron Microscopy showed large OC-like cells in the OC medium group. Micro-computed tomography showed increased formation in the OB medium group, equilibrium in the Neutral medium group and resorption in the OC medium group. Culture supernatant samples showed high early tartrate resistant acid phosphatase (TRAP) release in the OC medium group, a later and lower release in the Neutral medium group, and almost no release in the OB medium group. Increased monocyte seeding density showed a less-than-proportional increase in TRAP release and resorption in OC medium, while it proportionally increased TRAP release in Neutral medium without affecting net resorption. The 3D OB-OC co-culture model was effectively used to show an excess of mineral deposition using OB medium, resorption using OC medium, or an equilibrium using Neutral medium. All three media applied to the model may have their own distinct applications in fundamental research, drug development, and personalized medicine. |
| first_indexed | 2024-03-13T03:57:47Z |
| format | Article |
| id | doaj.art-3ddb3955cc724f598c5e2d0a3bfbb6c6 |
| institution | Directory Open Access Journal |
| issn | 2352-1872 |
| language | English |
| last_indexed | 2024-03-13T03:57:47Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Bone Reports |
| spelling | doaj.art-3ddb3955cc724f598c5e2d0a3bfbb6c62023-06-22T05:03:10ZengElsevierBone Reports2352-18722023-06-0118101646Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of boneStefan J.A. Remmers0Freek C. van der Heijden1Bregje W.M. de Wildt2Keita Ito3Sandra Hofmann4Orthopaedic Biomechanics, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the NetherlandsOrthopaedic Biomechanics, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the NetherlandsOrthopaedic Biomechanics, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the NetherlandsOrthopaedic Biomechanics, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the NetherlandsCorresponding author at: Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, the Netherlands.; Orthopaedic Biomechanics, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the NetherlandsThe aim of the present study was to further improve an in vitro 3D osteoblast (OB) – osteoclast (OC) co-culture model of bone by tuning it towards states of formation, resorption, and equilibrium for their future applications in fundamental research, drug development and personalized medicine. This was achieved by varying culture medium composition and monocyte seeding density, the two external parameters that affect cell behavior the most. Monocytes were seeded at two seeding densities onto 3D silk-fibroin constructs pre-mineralized by MSC-derived OBs and were co-cultured in one of three different media (OC stimulating, Neutral and OB stimulating medium) for three weeks. Histology showed mineralized matrix after co-culture and OC markers in the OC medium group. Scanning Electron Microscopy showed large OC-like cells in the OC medium group. Micro-computed tomography showed increased formation in the OB medium group, equilibrium in the Neutral medium group and resorption in the OC medium group. Culture supernatant samples showed high early tartrate resistant acid phosphatase (TRAP) release in the OC medium group, a later and lower release in the Neutral medium group, and almost no release in the OB medium group. Increased monocyte seeding density showed a less-than-proportional increase in TRAP release and resorption in OC medium, while it proportionally increased TRAP release in Neutral medium without affecting net resorption. The 3D OB-OC co-culture model was effectively used to show an excess of mineral deposition using OB medium, resorption using OC medium, or an equilibrium using Neutral medium. All three media applied to the model may have their own distinct applications in fundamental research, drug development, and personalized medicine.http://www.sciencedirect.com/science/article/pii/S2352187222004806OsteoblastOsteoclastCo-cultureRemodelingIn vitroCell culture |
| spellingShingle | Stefan J.A. Remmers Freek C. van der Heijden Bregje W.M. de Wildt Keita Ito Sandra Hofmann Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of bone Bone Reports Osteoblast Osteoclast Co-culture Remodeling In vitro Cell culture |
| title | Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of bone |
| title_full | Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of bone |
| title_fullStr | Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of bone |
| title_full_unstemmed | Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of bone |
| title_short | Tuning the resorption-formation balance in an in vitro 3D osteoblast-osteoclast co-culture model of bone |
| title_sort | tuning the resorption formation balance in an in vitro 3d osteoblast osteoclast co culture model of bone |
| topic | Osteoblast Osteoclast Co-culture Remodeling In vitro Cell culture |
| url | http://www.sciencedirect.com/science/article/pii/S2352187222004806 |
| work_keys_str_mv | AT stefanjaremmers tuningtheresorptionformationbalanceinaninvitro3dosteoblastosteoclastcoculturemodelofbone AT freekcvanderheijden tuningtheresorptionformationbalanceinaninvitro3dosteoblastosteoclastcoculturemodelofbone AT bregjewmdewildt tuningtheresorptionformationbalanceinaninvitro3dosteoblastosteoclastcoculturemodelofbone AT keitaito tuningtheresorptionformationbalanceinaninvitro3dosteoblastosteoclastcoculturemodelofbone AT sandrahofmann tuningtheresorptionformationbalanceinaninvitro3dosteoblastosteoclastcoculturemodelofbone |