Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence
Ornithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety of ornamentals. In this study, the coat protein (CP) gene of OrMVwas used to investigate the molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two groups which hav...
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PeerJ Inc.
2018-03-01
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| Online Access: | https://peerj.com/articles/4550.pdf |
| _version_ | 1797417931629395968 |
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| author | Fangluan Gao Zhenguo Du Jianguo Shen Hongkai Yang Furong Liao |
| author_facet | Fangluan Gao Zhenguo Du Jianguo Shen Hongkai Yang Furong Liao |
| author_sort | Fangluan Gao |
| collection | DOAJ |
| description | Ornithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety of ornamentals. In this study, the coat protein (CP) gene of OrMVwas used to investigate the molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two groups which have significant subpopulation differentiation with an FST value of 0.470. One isolate was identified as a recombinant and the other 35 recombination-free isolates could be divided into two major clades under different evolutionary constraints with dN/dS values of 0.055 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In addition, the results from analysis of molecular variance (AMOVA) indicated that the effect of host species on the genetic divergence of OrMV is greater than that of geography. Furthermore, OrMV isolates from the genera Ornithogalum, Lachenalia and Diuri tended to group together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation. |
| first_indexed | 2024-03-09T06:25:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:25:43Z |
| publishDate | 2018-03-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-3de0438e42254b98aa633fd652ea23b12023-12-03T11:19:57ZengPeerJ Inc.PeerJ2167-83592018-03-016e455010.7717/peerj.4550Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequenceFangluan Gao0Zhenguo Du1Jianguo Shen2Hongkai Yang3Furong Liao4Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaFujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaInspection and Quarantine Technology Center, Fujian Exit-Entry, Inspection and Quarantine Bureau, Fuzhou, Fujian, ChinaFujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaInspection and Quarantine Technology Center, Xiamen Exit-Entry Inspection and Quarantine Bureau, Xiamen, Fujian, ChinaOrnithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety of ornamentals. In this study, the coat protein (CP) gene of OrMVwas used to investigate the molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two groups which have significant subpopulation differentiation with an FST value of 0.470. One isolate was identified as a recombinant and the other 35 recombination-free isolates could be divided into two major clades under different evolutionary constraints with dN/dS values of 0.055 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In addition, the results from analysis of molecular variance (AMOVA) indicated that the effect of host species on the genetic divergence of OrMV is greater than that of geography. Furthermore, OrMV isolates from the genera Ornithogalum, Lachenalia and Diuri tended to group together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation.https://peerj.com/articles/4550.pdfPhylogenetic analysisHost-driven adaptationOrnithogalum mosaic virusSelective constraints |
| spellingShingle | Fangluan Gao Zhenguo Du Jianguo Shen Hongkai Yang Furong Liao Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence PeerJ Phylogenetic analysis Host-driven adaptation Ornithogalum mosaic virus Selective constraints |
| title | Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence |
| title_full | Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence |
| title_fullStr | Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence |
| title_full_unstemmed | Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence |
| title_short | Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence |
| title_sort | genetic diversity and molecular evolution of ornithogalum mosaic virus based on the coat protein gene sequence |
| topic | Phylogenetic analysis Host-driven adaptation Ornithogalum mosaic virus Selective constraints |
| url | https://peerj.com/articles/4550.pdf |
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