Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and Lactation
Chitosan oligosaccharide (COS) is derived through deacetylation of chitin from crustacean shells. Previous studies reported the benefits of COS to gut microbiota, immunity and health of host species. In this study, 120 pregnant composite ewes were subdivided into treatment and control groups in dupl...
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MDPI AG
2022-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/19/2609 |
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| author | Marefa Jahan Cara Wilson Shawn McGrath Nidhish Francis Peter C. Wynn Yuguang Du Bruce Allworth Bing Wang |
| author_facet | Marefa Jahan Cara Wilson Shawn McGrath Nidhish Francis Peter C. Wynn Yuguang Du Bruce Allworth Bing Wang |
| author_sort | Marefa Jahan |
| collection | DOAJ |
| description | Chitosan oligosaccharide (COS) is derived through deacetylation of chitin from crustacean shells. Previous studies reported the benefits of COS to gut microbiota, immunity and health of host species. In this study, 120 pregnant composite ewes were subdivided into treatment and control groups in duplicate. COS was supplemented via a loose lick to provide an estimated intake of COS @100–600 mg/d/ewe for five weeks pre-lambing until lamb marking. Body weight was recorded pre-treatment for ewes, and at lamb marking and weaning for both ewes and lambs. Serum immunity markers immunoglobulin G (IgG), immunoglobulin M (IgM), immunoglobulin A (IgA), secretory immunoglobulin A (sIgA), interleukin (IL)-2, IL10 and faecal sIgA were determined for ewes and lambs at lamb marking and weaning by enzyme-linked immunosorbent assay (ELISA). We found that COS can be incorporated in sheep feed without compromising palatability. Maternal COS supplementation did not influence the body weight of ewes or lambs. It did, however, significantly increase the concentrations of serum IL2 in ewes at marking and weaning (<i>p</i> < 0.001). In lambs, COS also significantly increased the IL2 concentration at making (<i>p</i> = 0.018) and weaning (<i>p</i> = 0.029) and serum IgM at marking (<i>p</i> < 0.001). No significant effect was observed in the concentration of any other immune marker or cytokine in either ewes or lambs. In conclusion, maternal COS supplementation significantly modulated some immunity markers in both ewes and lambs. The short duration of maternal COS supplementation and optimal seasonal conditions during the trial may explain the lack of significant body weight in ewes and lambs from the COS supplementation. |
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| last_indexed | 2024-03-09T22:08:17Z |
| publishDate | 2022-09-01 |
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| series | Animals |
| spelling | doaj.art-3de27ad5790c4e69b469463a686a13112023-11-23T19:36:36ZengMDPI AGAnimals2076-26152022-09-011219260910.3390/ani12192609Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and LactationMarefa Jahan0Cara Wilson1Shawn McGrath2Nidhish Francis3Peter C. Wynn4Yuguang Du5Bruce Allworth6Bing Wang7Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaGulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaGulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaSchool of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaGulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaState Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, ChinaGulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaGulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaChitosan oligosaccharide (COS) is derived through deacetylation of chitin from crustacean shells. Previous studies reported the benefits of COS to gut microbiota, immunity and health of host species. In this study, 120 pregnant composite ewes were subdivided into treatment and control groups in duplicate. COS was supplemented via a loose lick to provide an estimated intake of COS @100–600 mg/d/ewe for five weeks pre-lambing until lamb marking. Body weight was recorded pre-treatment for ewes, and at lamb marking and weaning for both ewes and lambs. Serum immunity markers immunoglobulin G (IgG), immunoglobulin M (IgM), immunoglobulin A (IgA), secretory immunoglobulin A (sIgA), interleukin (IL)-2, IL10 and faecal sIgA were determined for ewes and lambs at lamb marking and weaning by enzyme-linked immunosorbent assay (ELISA). We found that COS can be incorporated in sheep feed without compromising palatability. Maternal COS supplementation did not influence the body weight of ewes or lambs. It did, however, significantly increase the concentrations of serum IL2 in ewes at marking and weaning (<i>p</i> < 0.001). In lambs, COS also significantly increased the IL2 concentration at making (<i>p</i> = 0.018) and weaning (<i>p</i> = 0.029) and serum IgM at marking (<i>p</i> < 0.001). No significant effect was observed in the concentration of any other immune marker or cytokine in either ewes or lambs. In conclusion, maternal COS supplementation significantly modulated some immunity markers in both ewes and lambs. The short duration of maternal COS supplementation and optimal seasonal conditions during the trial may explain the lack of significant body weight in ewes and lambs from the COS supplementation.https://www.mdpi.com/2076-2615/12/19/2609Chitosan oligosaccharideewelambimmunitybody weight |
| spellingShingle | Marefa Jahan Cara Wilson Shawn McGrath Nidhish Francis Peter C. Wynn Yuguang Du Bruce Allworth Bing Wang Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and Lactation Animals Chitosan oligosaccharide ewe lamb immunity body weight |
| title | Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and Lactation |
| title_full | Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and Lactation |
| title_fullStr | Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and Lactation |
| title_full_unstemmed | Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and Lactation |
| title_short | Chitosan Oligosaccharide Supplementation Affects Immunity Markers in Ewes and Lambs during Gestation and Lactation |
| title_sort | chitosan oligosaccharide supplementation affects immunity markers in ewes and lambs during gestation and lactation |
| topic | Chitosan oligosaccharide ewe lamb immunity body weight |
| url | https://www.mdpi.com/2076-2615/12/19/2609 |
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