Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy
Decidual CD4+ T (dCD4 T) cells are crucial for the maternal-fetal immune tolerance required for a healthy pregnancy outcome. However, their molecular and functional characteristics are not well elucidated. In this study, we performed the first analysis of transcriptional and alternative splicing (AS...
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.00682/full |
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| author | Weihong Zeng Zhicui Liu Xinmei Liu Siming Zhang Asma Khanniche Ying Zheng Xiaoling Ma Tiantian Yu Fuju Tian Xiao-Rui Liu Jianxia Fan Yi Lin |
| author_facet | Weihong Zeng Zhicui Liu Xinmei Liu Siming Zhang Asma Khanniche Ying Zheng Xiaoling Ma Tiantian Yu Fuju Tian Xiao-Rui Liu Jianxia Fan Yi Lin |
| author_sort | Weihong Zeng |
| collection | DOAJ |
| description | Decidual CD4+ T (dCD4 T) cells are crucial for the maternal-fetal immune tolerance required for a healthy pregnancy outcome. However, their molecular and functional characteristics are not well elucidated. In this study, we performed the first analysis of transcriptional and alternative splicing (AS) landscapes for paired decidual and peripheral blood CD4+ T (pCD4 T) cells in human early pregnancy using high throughput mRNA sequencing. Our data showed that dCD4 T cells are endowed with a unique transcriptional signature when compared to pCD4 T cells: dCD4 T cells upregulate 1,695 genes enriched in immune system process whereas downregulate 1,011 genes mainly related to mRNA catabolic process and the ribosome. Moreover, dCD4 T cells were observed to be at M phase, and show increased activation, proliferation, and cytokine production, as well as display an effector-memory phenotype and a heterogenous nature containing Th1, Th17, and Treg cell subsets. However, dCD4 T cells undergo a comparable number of upregulated and downregulated AS events, both of which are enriched in the genes related to cellular metabolic process. And the changes at the AS event level do not reflect measurable differences at the gene expression level in dCD4 T cells. Collectively, our findings provide a comprehensive portrait of the unique transcriptional signature and AS profile of CD4+ T cells in human decidua and help us gain more understanding of the functional characteristic of these cells during early pregnancy. |
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| language | English |
| last_indexed | 2024-04-12T17:28:01Z |
| publishDate | 2017-06-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-3de2f9ee5a9b47218e2a915fe5ee254f2022-12-22T03:23:14ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-06-01810.3389/fimmu.2017.00682264741Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human PregnancyWeihong Zeng0Zhicui Liu1Xinmei Liu2Siming Zhang3Asma Khanniche4Ying Zheng5Xiaoling Ma6Tiantian Yu7Fuju Tian8Xiao-Rui Liu9Jianxia Fan10Yi Lin11Institute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Dermatology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaOut-patient Operating Room, The International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDecidual CD4+ T (dCD4 T) cells are crucial for the maternal-fetal immune tolerance required for a healthy pregnancy outcome. However, their molecular and functional characteristics are not well elucidated. In this study, we performed the first analysis of transcriptional and alternative splicing (AS) landscapes for paired decidual and peripheral blood CD4+ T (pCD4 T) cells in human early pregnancy using high throughput mRNA sequencing. Our data showed that dCD4 T cells are endowed with a unique transcriptional signature when compared to pCD4 T cells: dCD4 T cells upregulate 1,695 genes enriched in immune system process whereas downregulate 1,011 genes mainly related to mRNA catabolic process and the ribosome. Moreover, dCD4 T cells were observed to be at M phase, and show increased activation, proliferation, and cytokine production, as well as display an effector-memory phenotype and a heterogenous nature containing Th1, Th17, and Treg cell subsets. However, dCD4 T cells undergo a comparable number of upregulated and downregulated AS events, both of which are enriched in the genes related to cellular metabolic process. And the changes at the AS event level do not reflect measurable differences at the gene expression level in dCD4 T cells. Collectively, our findings provide a comprehensive portrait of the unique transcriptional signature and AS profile of CD4+ T cells in human decidua and help us gain more understanding of the functional characteristic of these cells during early pregnancy.http://journal.frontiersin.org/article/10.3389/fimmu.2017.00682/fulldecidual CD4+ T cellsearly human pregnancytranscriptomealternative splicinghigh-throughput mRNA sequencing |
| spellingShingle | Weihong Zeng Zhicui Liu Xinmei Liu Siming Zhang Asma Khanniche Ying Zheng Xiaoling Ma Tiantian Yu Fuju Tian Xiao-Rui Liu Jianxia Fan Yi Lin Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy Frontiers in Immunology decidual CD4+ T cells early human pregnancy transcriptome alternative splicing high-throughput mRNA sequencing |
| title | Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy |
| title_full | Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy |
| title_fullStr | Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy |
| title_full_unstemmed | Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy |
| title_short | Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy |
| title_sort | distinct transcriptional and alternative splicing signatures of decidual cd4 t cells in early human pregnancy |
| topic | decidual CD4+ T cells early human pregnancy transcriptome alternative splicing high-throughput mRNA sequencing |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2017.00682/full |
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