Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions
Colorectal cancer is the third most common form of cancer and the second leading cause of cancer-related death in the Western world. The answers to such life threatening diseases and cancers are monoclonal antibodies (MAb's) which are widely used as therapeutic agents. World demand for currentl...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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IIUM Press, International Islamic University Malaysia
2011-12-01
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| Series: | International Islamic University Malaysia Engineering Journal |
| Online Access: | http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/265 |
| _version_ | 1818363487754125312 |
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| author | Junaid Muneer Raja Nurina Anuar and Badarulhisam Abdul Rahman |
| author_facet | Junaid Muneer Raja Nurina Anuar and Badarulhisam Abdul Rahman |
| author_sort | Junaid Muneer Raja |
| collection | DOAJ |
| description | Colorectal cancer is the third most common form of cancer and the second leading cause of cancer-related death in the Western world. The answers to such life threatening diseases and cancers are monoclonal antibodies (MAb's) which are widely used as therapeutic agents. World demand for currently approved MAb's is on the order of a few kilograms per year. However, new therapeutic MAb's are under development and require doses of several hundred milligrams to a gram over the course of therapy. Very often to cater for the special requirements for the growth of mammalian cells, serum is added to the cell culture medium. However, removal of serum from the cell culture medium is often carried out, especially if the end product is to be used for human consumption, in order to eliminate various disadvantages such as high physiological variability, high batch to batch variability, risk of contamination and high cost, and challenges posed in the downstream processing of the product. In this paper, the adaptation of cholesterol requiring NS0 cells to commercially available serum free media is presented.
ABSTRAK: Kanser kolorektum merupakan kanser ketiga paling umum dan kini berada di tempat kedua penyebab kematian berkaitan kanser di negara Barat. Jawapan kepada penyakit yang mengancam nyawa dan penyakit kanser adalah antibodi monoklon (monoclonal antibodies ((MAb's)) yang digunakan sebagai agen terapeutik. Permintaan dunia terhadap MAb's yang diluluskan adalah dalam bilangan beberapa kilogram setahun. Namun, terapeutik MAb's yang baru adalah di bawah penyelidikan dan memerlukan beberapa ratus dos milligram hingga satu gram dalam satu peringkat terapi. Sering kali untuk memenuhi permintaan terhadap tumbesaran sel mamalia, serum dicampurkan dengan sel kultur perantara. Walaupun begitu, pemindahan serum dari sel kultur perantara sering dilakukan, terutamanya jika produk akhir digunakan untuk kegunaan manusia; untuk mengurangkan pelbagai kelemahan seperti kebolehubahan psikologi yang tinggi, kebolehubahan yang tinggi daripada satu kumpulan ke satu kumpulan lain, risiko pencemaran, kos yang tinggi, dan cabaran mendatang dalam pemprosesan produk. Dalam perbentangan ini, kolestrol yang diubah memerlukan sel NS0 yang dikomersilkan dengan serum bebas perantara. |
| first_indexed | 2024-12-13T21:49:16Z |
| format | Article |
| id | doaj.art-3de69878b88b4d0796dde5230688fb40 |
| institution | Directory Open Access Journal |
| issn | 1511-788X 2289-7860 |
| language | English |
| last_indexed | 2024-12-13T21:49:16Z |
| publishDate | 2011-12-01 |
| publisher | IIUM Press, International Islamic University Malaysia |
| record_format | Article |
| series | International Islamic University Malaysia Engineering Journal |
| spelling | doaj.art-3de69878b88b4d0796dde5230688fb402022-12-21T23:30:19ZengIIUM Press, International Islamic University MalaysiaInternational Islamic University Malaysia Engineering Journal1511-788X2289-78602011-12-0112410.31436/iiumej.v12i4.265Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture ConditionsJunaid Muneer Raja0Nurina Anuarand Badarulhisam Abdul RahmanDevelopment Engineer - InnoBiologics Sdn BhdColorectal cancer is the third most common form of cancer and the second leading cause of cancer-related death in the Western world. The answers to such life threatening diseases and cancers are monoclonal antibodies (MAb's) which are widely used as therapeutic agents. World demand for currently approved MAb's is on the order of a few kilograms per year. However, new therapeutic MAb's are under development and require doses of several hundred milligrams to a gram over the course of therapy. Very often to cater for the special requirements for the growth of mammalian cells, serum is added to the cell culture medium. However, removal of serum from the cell culture medium is often carried out, especially if the end product is to be used for human consumption, in order to eliminate various disadvantages such as high physiological variability, high batch to batch variability, risk of contamination and high cost, and challenges posed in the downstream processing of the product. In this paper, the adaptation of cholesterol requiring NS0 cells to commercially available serum free media is presented. ABSTRAK: Kanser kolorektum merupakan kanser ketiga paling umum dan kini berada di tempat kedua penyebab kematian berkaitan kanser di negara Barat. Jawapan kepada penyakit yang mengancam nyawa dan penyakit kanser adalah antibodi monoklon (monoclonal antibodies ((MAb's)) yang digunakan sebagai agen terapeutik. Permintaan dunia terhadap MAb's yang diluluskan adalah dalam bilangan beberapa kilogram setahun. Namun, terapeutik MAb's yang baru adalah di bawah penyelidikan dan memerlukan beberapa ratus dos milligram hingga satu gram dalam satu peringkat terapi. Sering kali untuk memenuhi permintaan terhadap tumbesaran sel mamalia, serum dicampurkan dengan sel kultur perantara. Walaupun begitu, pemindahan serum dari sel kultur perantara sering dilakukan, terutamanya jika produk akhir digunakan untuk kegunaan manusia; untuk mengurangkan pelbagai kelemahan seperti kebolehubahan psikologi yang tinggi, kebolehubahan yang tinggi daripada satu kumpulan ke satu kumpulan lain, risiko pencemaran, kos yang tinggi, dan cabaran mendatang dalam pemprosesan produk. Dalam perbentangan ini, kolestrol yang diubah memerlukan sel NS0 yang dikomersilkan dengan serum bebas perantara.http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/265 |
| spellingShingle | Junaid Muneer Raja Nurina Anuar and Badarulhisam Abdul Rahman Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions International Islamic University Malaysia Engineering Journal |
| title | Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions |
| title_full | Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions |
| title_fullStr | Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions |
| title_full_unstemmed | Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions |
| title_short | Adaptation of Cholesterol Requiring NS0 Cells to Serum Free Culture Conditions |
| title_sort | adaptation of cholesterol requiring ns0 cells to serum free culture conditions |
| url | http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/265 |
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