Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications
Perinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers an...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.958669/full |
| _version_ | 1828105405007396864 |
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| author | Antonietta R. Silini Taja Železnik Ramuta Ana Salomé Pires Ana Salomé Pires Ana Salomé Pires Asmita Banerjee Marie Dubus Florelle Gindraux Halima Kerdjoudj Justinas Maciulatis Adelheid Weidinger Susanne Wolbank Günther Eissner Bernd Giebel Michela Pozzobon Ornella Parolini Ornella Parolini Mateja Erdani Kreft |
| author_facet | Antonietta R. Silini Taja Železnik Ramuta Ana Salomé Pires Ana Salomé Pires Ana Salomé Pires Asmita Banerjee Marie Dubus Florelle Gindraux Halima Kerdjoudj Justinas Maciulatis Adelheid Weidinger Susanne Wolbank Günther Eissner Bernd Giebel Michela Pozzobon Ornella Parolini Ornella Parolini Mateja Erdani Kreft |
| author_sort | Antonietta R. Silini |
| collection | DOAJ |
| description | Perinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers and infections. Indeed, there is growing evidence that PnDs possess anticancer and antimicrobial activities, but an urgent issue that needs to be addressed is the reproducible evaluation of efficacy, both in vitro and in vivo. Herein we present the most commonly used functional assays for the assessment of antitumor and antimicrobial properties of PnDs, and we discuss their advantages and disadvantages in assessing the functionality. This review is part of a quadrinomial series on functional assays for the validation of PnDs spanning biological functions such as immunomodulation, anticancer and antimicrobial, wound healing, and regeneration. |
| first_indexed | 2024-04-11T10:02:43Z |
| format | Article |
| id | doaj.art-3de6aa08611d44ec8f53d7605ecaa0a9 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T10:02:43Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-3de6aa08611d44ec8f53d7605ecaa0a92022-12-22T04:30:19ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-10-011010.3389/fbioe.2022.958669958669Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applicationsAntonietta R. Silini0Taja Železnik Ramuta1Ana Salomé Pires2Ana Salomé Pires3Ana Salomé Pires4Asmita Banerjee5Marie Dubus6Florelle Gindraux7Halima Kerdjoudj8Justinas Maciulatis9Adelheid Weidinger10Susanne Wolbank11Günther Eissner12Bernd Giebel13Michela Pozzobon14Ornella Parolini15Ornella Parolini16Mateja Erdani Kreft17Centro di Ricerca E. Menni, Fondazione Poliambulanza Istituto Ospedaliero, Brescia, ItalyFaculty of Medicine, Institute of Cell Biology, University of Ljubljana, Ljubljana, SloveniaFaculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR) Area of Environment, Genetics and Oncobiology (CIMAGO), Institute of Biophysics, University of Coimbra, Coimbra, PortugalCenter for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, PortugalClinical Academic Center of Coimbra (CACC), Coimbra, PortugalLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Austrian Cluster for Tissue Regeneration, Vienna, AustriaUniversité de Reims Champagne Ardenne, EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), Reims, FranceService de Chirurgie Orthopédique, Traumatologique et Plastique, CHU Besançon and Laboratoire de Nanomédecine, Imagerie, Thérapeutique EA 4662, Université Bourgogne Franche-Comté, Besançon, FranceUniversité de Reims Champagne Ardenne, EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), Reims, FranceThe Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, LithuaniaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Austrian Cluster for Tissue Regeneration, Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Austrian Cluster for Tissue Regeneration, Vienna, Austria0Systems Biology Ireland, UCD School of Medicine, University College Dublin, Dublin, Ireland1Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany2Stem Cells and Regenerative Medicine Lab, Department of Women’s and Children’s Health, University of Padova, Fondazione Istituto di Ricerca Pediatrica Città Della Speranza, Padoa, Italy3Department of Life Science and Public Health, Università Cattolica Del Sacro Cuore, Rome, Italy4Fondazione Policlinico Universitario “Agostino Gemelli” IRCCS, Rome, ItalyFaculty of Medicine, Institute of Cell Biology, University of Ljubljana, Ljubljana, SloveniaPerinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers and infections. Indeed, there is growing evidence that PnDs possess anticancer and antimicrobial activities, but an urgent issue that needs to be addressed is the reproducible evaluation of efficacy, both in vitro and in vivo. Herein we present the most commonly used functional assays for the assessment of antitumor and antimicrobial properties of PnDs, and we discuss their advantages and disadvantages in assessing the functionality. This review is part of a quadrinomial series on functional assays for the validation of PnDs spanning biological functions such as immunomodulation, anticancer and antimicrobial, wound healing, and regeneration.https://www.frontiersin.org/articles/10.3389/fbioe.2022.958669/fullperinatal derivativesbiological assaysfunctional assayspotency assaysmechanisms of actionpharmacologic actions |
| spellingShingle | Antonietta R. Silini Taja Železnik Ramuta Ana Salomé Pires Ana Salomé Pires Ana Salomé Pires Asmita Banerjee Marie Dubus Florelle Gindraux Halima Kerdjoudj Justinas Maciulatis Adelheid Weidinger Susanne Wolbank Günther Eissner Bernd Giebel Michela Pozzobon Ornella Parolini Ornella Parolini Mateja Erdani Kreft Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications Frontiers in Bioengineering and Biotechnology perinatal derivatives biological assays functional assays potency assays mechanisms of action pharmacologic actions |
| title | Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications |
| title_full | Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications |
| title_fullStr | Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications |
| title_full_unstemmed | Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications |
| title_short | Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications |
| title_sort | methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications |
| topic | perinatal derivatives biological assays functional assays potency assays mechanisms of action pharmacologic actions |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.958669/full |
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