Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry Carcasses
The impact of soil with an intact microbial community and oxygen availability on moisture content, soil pH, and bacterial communities during decomposition of poultry carcasses was investigated. Poultry carcasses were decomposed in soil with or without a microbial community, under aerobic or anaerobi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/10/2937 |
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| author | Michelle A. Miguel Seon-Ho Kim Sang-Suk Lee Yong-Il Cho |
| author_facet | Michelle A. Miguel Seon-Ho Kim Sang-Suk Lee Yong-Il Cho |
| author_sort | Michelle A. Miguel |
| collection | DOAJ |
| description | The impact of soil with an intact microbial community and oxygen availability on moisture content, soil pH, and bacterial communities during decomposition of poultry carcasses was investigated. Poultry carcasses were decomposed in soil with or without a microbial community, under aerobic or anaerobic conditions. The samples collected in each microcosm burial set-up were analyzed by targeted 16S rRNA amplicon sequencing and Amplicon sequence variants (ASV) method. Our results showed that moisture was high in the burial set-ups under anaerobic conditions and pH was high in the burial set-ups under aerobic conditions. Meanwhile, the Chao1 and Shannon index significantly differed between the different burial set-ups and across different time points. In addition, bacterial taxa composition during the early period of decomposition differed from that of the late period. A total of 23 phyla, 901 genera, and 1992 species were identified. Firmicutes was the most dominant phyla in all burial set-ups throughout the decomposition. At day 60, <i>Pseudogracilibacillus</i> was dominant in the burial set-ups under aerobic conditions, while <i>Lentibacillus</i> dominated in the burial set-ups under anaerobic conditions. This study demonstrated that the soil microbial community and availability of oxygen significantly affected the changes in moisture content, pH, and bacterial composition during the decomposition process. |
| first_indexed | 2024-03-10T06:46:05Z |
| format | Article |
| id | doaj.art-3de94cf23b384f1eaa3ac8f6ed59eb17 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T06:46:05Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-3de94cf23b384f1eaa3ac8f6ed59eb172023-11-22T17:11:32ZengMDPI AGAnimals2076-26152021-10-011110293710.3390/ani11102937Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry CarcassesMichelle A. Miguel0Seon-Ho Kim1Sang-Suk Lee2Yong-Il Cho3Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, KoreaDepartment of Animal Science and Technology, Sunchon National University, Suncheon 57922, KoreaDepartment of Animal Science and Technology, Sunchon National University, Suncheon 57922, KoreaDepartment of Animal Science and Technology, Sunchon National University, Suncheon 57922, KoreaThe impact of soil with an intact microbial community and oxygen availability on moisture content, soil pH, and bacterial communities during decomposition of poultry carcasses was investigated. Poultry carcasses were decomposed in soil with or without a microbial community, under aerobic or anaerobic conditions. The samples collected in each microcosm burial set-up were analyzed by targeted 16S rRNA amplicon sequencing and Amplicon sequence variants (ASV) method. Our results showed that moisture was high in the burial set-ups under anaerobic conditions and pH was high in the burial set-ups under aerobic conditions. Meanwhile, the Chao1 and Shannon index significantly differed between the different burial set-ups and across different time points. In addition, bacterial taxa composition during the early period of decomposition differed from that of the late period. A total of 23 phyla, 901 genera, and 1992 species were identified. Firmicutes was the most dominant phyla in all burial set-ups throughout the decomposition. At day 60, <i>Pseudogracilibacillus</i> was dominant in the burial set-ups under aerobic conditions, while <i>Lentibacillus</i> dominated in the burial set-ups under anaerobic conditions. This study demonstrated that the soil microbial community and availability of oxygen significantly affected the changes in moisture content, pH, and bacterial composition during the decomposition process.https://www.mdpi.com/2076-2615/11/10/293716S rRNAASVbacterial diversitycarcassdecompositionMiSeq |
| spellingShingle | Michelle A. Miguel Seon-Ho Kim Sang-Suk Lee Yong-Il Cho Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry Carcasses Animals 16S rRNA ASV bacterial diversity carcass decomposition MiSeq |
| title | Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry Carcasses |
| title_full | Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry Carcasses |
| title_fullStr | Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry Carcasses |
| title_full_unstemmed | Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry Carcasses |
| title_short | Impact of Soil Microbes and Oxygen Availability on Bacterial Community Structure of Decomposing Poultry Carcasses |
| title_sort | impact of soil microbes and oxygen availability on bacterial community structure of decomposing poultry carcasses |
| topic | 16S rRNA ASV bacterial diversity carcass decomposition MiSeq |
| url | https://www.mdpi.com/2076-2615/11/10/2937 |
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