Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount
The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid co...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Analytica |
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| Online Access: | https://www.mdpi.com/2673-4532/2/3/8 |
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| author | Toshiki Horikoshi Chihiro Kitaoka Yosuke Fujii Takashi Asano Jiawei Xu Tatsuya Fujino |
| author_facet | Toshiki Horikoshi Chihiro Kitaoka Yosuke Fujii Takashi Asano Jiawei Xu Tatsuya Fujino |
| author_sort | Toshiki Horikoshi |
| collection | DOAJ |
| description | The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H]<sup>+</sup> peak started later than that of [AAP+H]<sup>+</sup> peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis. |
| first_indexed | 2024-03-10T07:57:55Z |
| format | Article |
| id | doaj.art-3dea63d09a3c4496ac9df7b967e2bb3e |
| institution | Directory Open Access Journal |
| issn | 2673-4532 |
| language | English |
| last_indexed | 2024-03-10T07:57:55Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Analytica |
| spelling | doaj.art-3dea63d09a3c4496ac9df7b967e2bb3e2023-11-22T11:40:39ZengMDPI AGAnalytica2673-45322021-08-0123667510.3390/analytica2030008Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion AmountToshiki Horikoshi0Chihiro Kitaoka1Yosuke Fujii2Takashi Asano3Jiawei Xu4Tatsuya Fujino5Department of Applied Chemistry, Graduate School of Science and Engineering, Toyo University, 2100 Kujirai, Kawagoe 350-8585, JapanCriminal Investigation Laboratory, Metropolitan Police Department, 2-1-1 Kasumigaseki, Chiyoda-ku, Tokyo 100-8929, JapanDepartment of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0379, JapanForensic Science Laboratory, Metropolitan Police Department, 2-1-1 Kasumigaseki, Chiyoda-ku, Tokyo 100-8929, JapanDepartment of Applied Chemistry, Graduate School of Science and Engineering, Toyo University, 2100 Kujirai, Kawagoe 350-8585, JapanDepartment of Applied Chemistry, Graduate School of Science and Engineering, Toyo University, 2100 Kujirai, Kawagoe 350-8585, JapanThe ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H]<sup>+</sup> peak started later than that of [AAP+H]<sup>+</sup> peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis.https://www.mdpi.com/2673-4532/2/3/8matrix-assisted laser desorption ionization (MALDI)surface-assisted laser desorption ionization (SALDI)fingerprintacetaminophenzeolite |
| spellingShingle | Toshiki Horikoshi Chihiro Kitaoka Yosuke Fujii Takashi Asano Jiawei Xu Tatsuya Fujino Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount Analytica matrix-assisted laser desorption ionization (MALDI) surface-assisted laser desorption ionization (SALDI) fingerprint acetaminophen zeolite |
| title | Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount |
| title_full | Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount |
| title_fullStr | Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount |
| title_full_unstemmed | Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount |
| title_short | Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount |
| title_sort | detection of acetaminophen and its glucuronide in fingerprint by saldi mass spectrometry using zeolite and study of time dependent changes in detected ion amount |
| topic | matrix-assisted laser desorption ionization (MALDI) surface-assisted laser desorption ionization (SALDI) fingerprint acetaminophen zeolite |
| url | https://www.mdpi.com/2673-4532/2/3/8 |
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