Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana.
In eukaryotes, protein kinases catalyze the transfer of a gamma-phosphate from ATP (or GTP) to specific amino acids in protein targets. In plants, protein kinases have been shown to participate in signaling cascades driving responses to environmental stimuli and developmental processes. Plant merist...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3458904?pdf=render |
| _version_ | 1819162095591096320 |
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| author | Eduardo Alves Gamosa de Oliveira Nelilma Correia Romeiro Elane da Silva Ribeiro Claudete Santa-Catarina Antônia Elenir Amâncio Oliveira Vanildo Silveira Gonçalo Apolinário de Souza Filho Thiago Motta Venancio Marco Antônio Lopes Cruz |
| author_facet | Eduardo Alves Gamosa de Oliveira Nelilma Correia Romeiro Elane da Silva Ribeiro Claudete Santa-Catarina Antônia Elenir Amâncio Oliveira Vanildo Silveira Gonçalo Apolinário de Souza Filho Thiago Motta Venancio Marco Antônio Lopes Cruz |
| author_sort | Eduardo Alves Gamosa de Oliveira |
| collection | DOAJ |
| description | In eukaryotes, protein kinases catalyze the transfer of a gamma-phosphate from ATP (or GTP) to specific amino acids in protein targets. In plants, protein kinases have been shown to participate in signaling cascades driving responses to environmental stimuli and developmental processes. Plant meristems are undifferentiated tissues that provide the major source of cells that will form organs throughout development. However, non-dividing specialized cells can also dedifferentiate and re-initiate cell division if exposed to appropriate conditions. Mps1 (Monopolar spindle) is a dual-specificity protein kinase that plays a critical role in monitoring the accuracy of chromosome segregation in the mitotic checkpoint mechanism. Although Mps1 functions have been clearly demonstrated in animals and fungi, its role in plants is so far unclear. Here, using structural and biochemical analyses here we show that Mps1 has highly similar homologs in many plant genomes across distinct lineages (e.g. AtMps1 in Arabidopsis thaliana). Several structural features (i.e. catalytic site, DFG motif and threonine triad) are clearly conserved in plant Mps1 kinases. Structural and sequence analysis also suggest that AtMps1 interact with other cell cycle proteins, such as Mad2 and MAPK1. By using a very specific Mps1 inhibitor (SP600125) we show that compromised AtMps1 activity hampers the development of A. thaliana seedlings in a dose-dependent manner, especially in secondary roots. Moreover, concomitant administration of the auxin IAA neutralizes the AtMps1 inhibition phenotype, allowing secondary root development. These observations let us to hypothesize that AtMps1 might be a downstream regulator of IAA signaling in the formation of secondary roots. Our results indicate that Mps1 might be a universal component of the Spindle Assembly Checkpoint machinery across very distant lineages of eukaryotes. |
| first_indexed | 2024-12-22T17:22:47Z |
| format | Article |
| id | doaj.art-3df48ddaa3b94271b903ee656abe6bd4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:22:47Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-3df48ddaa3b94271b903ee656abe6bd42022-12-21T18:18:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4570710.1371/journal.pone.0045707Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana.Eduardo Alves Gamosa de OliveiraNelilma Correia RomeiroElane da Silva RibeiroClaudete Santa-CatarinaAntônia Elenir Amâncio OliveiraVanildo SilveiraGonçalo Apolinário de Souza FilhoThiago Motta VenancioMarco Antônio Lopes CruzIn eukaryotes, protein kinases catalyze the transfer of a gamma-phosphate from ATP (or GTP) to specific amino acids in protein targets. In plants, protein kinases have been shown to participate in signaling cascades driving responses to environmental stimuli and developmental processes. Plant meristems are undifferentiated tissues that provide the major source of cells that will form organs throughout development. However, non-dividing specialized cells can also dedifferentiate and re-initiate cell division if exposed to appropriate conditions. Mps1 (Monopolar spindle) is a dual-specificity protein kinase that plays a critical role in monitoring the accuracy of chromosome segregation in the mitotic checkpoint mechanism. Although Mps1 functions have been clearly demonstrated in animals and fungi, its role in plants is so far unclear. Here, using structural and biochemical analyses here we show that Mps1 has highly similar homologs in many plant genomes across distinct lineages (e.g. AtMps1 in Arabidopsis thaliana). Several structural features (i.e. catalytic site, DFG motif and threonine triad) are clearly conserved in plant Mps1 kinases. Structural and sequence analysis also suggest that AtMps1 interact with other cell cycle proteins, such as Mad2 and MAPK1. By using a very specific Mps1 inhibitor (SP600125) we show that compromised AtMps1 activity hampers the development of A. thaliana seedlings in a dose-dependent manner, especially in secondary roots. Moreover, concomitant administration of the auxin IAA neutralizes the AtMps1 inhibition phenotype, allowing secondary root development. These observations let us to hypothesize that AtMps1 might be a downstream regulator of IAA signaling in the formation of secondary roots. Our results indicate that Mps1 might be a universal component of the Spindle Assembly Checkpoint machinery across very distant lineages of eukaryotes.http://europepmc.org/articles/PMC3458904?pdf=render |
| spellingShingle | Eduardo Alves Gamosa de Oliveira Nelilma Correia Romeiro Elane da Silva Ribeiro Claudete Santa-Catarina Antônia Elenir Amâncio Oliveira Vanildo Silveira Gonçalo Apolinário de Souza Filho Thiago Motta Venancio Marco Antônio Lopes Cruz Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana. PLoS ONE |
| title | Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana. |
| title_full | Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana. |
| title_fullStr | Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana. |
| title_full_unstemmed | Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana. |
| title_short | Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana. |
| title_sort | structural and functional characterization of the protein kinase mps1 in arabidopsis thaliana |
| url | http://europepmc.org/articles/PMC3458904?pdf=render |
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