Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.)
Spodoptera litura (Fabricius) is an agriculturally significant polyphagous insect pest that has evolved a high level of resistance to conventional insecticides. A dietary assay was used in this work to assess the resilience of field populations of S. litura to λ-cyhalothrin. Analysis of the function...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.900570/full |
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| author | Narayanan Shyam-Sundar Ramakrishnan Ramasubramanian Sengodan Karthi Sengodan Karthi Sengottayan Senthil-Nathan Kanagaraj Muthu-Pandian Chanthini Haridoss Sivanesh Vethamonickam Stanley-Raja Govindaraju Ramkumar Kilapavoor Raman Narayanan Shahid Mahboob Khalid Abdullah Al-Ghanim Ahmed Abdel-Megeed Patcharin Krutmuang Patcharin Krutmuang |
| author_facet | Narayanan Shyam-Sundar Ramakrishnan Ramasubramanian Sengodan Karthi Sengodan Karthi Sengottayan Senthil-Nathan Kanagaraj Muthu-Pandian Chanthini Haridoss Sivanesh Vethamonickam Stanley-Raja Govindaraju Ramkumar Kilapavoor Raman Narayanan Shahid Mahboob Khalid Abdullah Al-Ghanim Ahmed Abdel-Megeed Patcharin Krutmuang Patcharin Krutmuang |
| author_sort | Narayanan Shyam-Sundar |
| collection | DOAJ |
| description | Spodoptera litura (Fabricius) is an agriculturally significant polyphagous insect pest that has evolved a high level of resistance to conventional insecticides. A dietary assay was used in this work to assess the resilience of field populations of S. litura to λ-cyhalothrin. Analysis of the function and expression of the cytochrome P450 gene was used to test the sensitivity of S. litura larvae to sub-lethal concentrations of the insecticidal plant chemical Precocene 1, both by itself and in combination with λ-cyhalothrin. The activity of esterase enzymes (α and β) was found to decrease 48 h post treatment with Precocene 1. The activity of GST enzyme and cytochrome P450 increased with Precocene 1 treatment post 48 h, however. Expression studies revealed the modulation by Precocene 1 of cytochrome P450 genes, CYP4M16, CYP4M15, CYP4S8V4, CYP4G31, and CYP4L10. While CYP4M16 expression was stimulated the most by the synergistic Precocene 1 + λ–cyhalothrin treatment, expression of CYP4G31 was the most down-regulated by Precocene 1 exposure. Hence, it is evident that λ–cyhalothrin-resistant pest populations are still sensitive to Precocene 1 at a sublethal concentration that is nevertheless capable of hindering their development. Precocene 1 can therefore be considered a potent candidate for the effective management of insecticide-resilient S. litura. |
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| language | English |
| last_indexed | 2024-04-11T08:11:21Z |
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| series | Frontiers in Physiology |
| spelling | doaj.art-3df490531451415fb6897930008e54ad2022-12-22T04:35:19ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-11-011310.3389/fphys.2022.900570900570Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.)Narayanan Shyam-Sundar0Ramakrishnan Ramasubramanian1Sengodan Karthi2Sengodan Karthi3Sengottayan Senthil-Nathan4Kanagaraj Muthu-Pandian Chanthini5Haridoss Sivanesh6Vethamonickam Stanley-Raja7Govindaraju Ramkumar8Kilapavoor Raman Narayanan9Shahid Mahboob10Khalid Abdullah Al-Ghanim11Ahmed Abdel-Megeed12Patcharin Krutmuang13Patcharin Krutmuang14Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDepartment of Entomology, University of Kentucky, Lexington, KY, United StatesDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, IndiaDepartment of Zoology, Sri Paramakalyani College, Tirunelveli, Tamil Nadu, IndiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Plant Protection, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, EgyptDepartment of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai, ThailandInnovative Agriculture Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, ThailandSpodoptera litura (Fabricius) is an agriculturally significant polyphagous insect pest that has evolved a high level of resistance to conventional insecticides. A dietary assay was used in this work to assess the resilience of field populations of S. litura to λ-cyhalothrin. Analysis of the function and expression of the cytochrome P450 gene was used to test the sensitivity of S. litura larvae to sub-lethal concentrations of the insecticidal plant chemical Precocene 1, both by itself and in combination with λ-cyhalothrin. The activity of esterase enzymes (α and β) was found to decrease 48 h post treatment with Precocene 1. The activity of GST enzyme and cytochrome P450 increased with Precocene 1 treatment post 48 h, however. Expression studies revealed the modulation by Precocene 1 of cytochrome P450 genes, CYP4M16, CYP4M15, CYP4S8V4, CYP4G31, and CYP4L10. While CYP4M16 expression was stimulated the most by the synergistic Precocene 1 + λ–cyhalothrin treatment, expression of CYP4G31 was the most down-regulated by Precocene 1 exposure. Hence, it is evident that λ–cyhalothrin-resistant pest populations are still sensitive to Precocene 1 at a sublethal concentration that is nevertheless capable of hindering their development. Precocene 1 can therefore be considered a potent candidate for the effective management of insecticide-resilient S. litura.https://www.frontiersin.org/articles/10.3389/fphys.2022.900570/fullphytochemicalsPrecocene 1pyrethroidgrowth regulatoranti-juvenile hormonecytochrome P450 |
| spellingShingle | Narayanan Shyam-Sundar Ramakrishnan Ramasubramanian Sengodan Karthi Sengodan Karthi Sengottayan Senthil-Nathan Kanagaraj Muthu-Pandian Chanthini Haridoss Sivanesh Vethamonickam Stanley-Raja Govindaraju Ramkumar Kilapavoor Raman Narayanan Shahid Mahboob Khalid Abdullah Al-Ghanim Ahmed Abdel-Megeed Patcharin Krutmuang Patcharin Krutmuang Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.) Frontiers in Physiology phytochemicals Precocene 1 pyrethroid growth regulator anti-juvenile hormone cytochrome P450 |
| title | Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.) |
| title_full | Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.) |
| title_fullStr | Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.) |
| title_full_unstemmed | Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.) |
| title_short | Effects of phytocompound Precocene 1 on the expression and functionality of the P450 gene in λ-cyhalothrin-resistant Spodoptera litura (Fab.) |
| title_sort | effects of phytocompound precocene 1 on the expression and functionality of the p450 gene in λ cyhalothrin resistant spodoptera litura fab |
| topic | phytochemicals Precocene 1 pyrethroid growth regulator anti-juvenile hormone cytochrome P450 |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.900570/full |
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