A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products
Lead contamination in aquatic products is one of the main hazard factors. The aptasensor is a promising detection method for lead ion (Pb(II)) because of its selectivity, but it is easily affected by pH. The combination of ion-imprinted polymers(IIP) with aptamers may improve their stability in diff...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/1/196 |
| _version_ | 1797440323924787200 |
|---|---|
| author | Nianxin Zhu Xinna Liu Kaimin Peng Hui Cao Min Yuan Tai Ye Xiuxiu Wu Fengqin Yin Jinsong Yu Liling Hao Fei Xu |
| author_facet | Nianxin Zhu Xinna Liu Kaimin Peng Hui Cao Min Yuan Tai Ye Xiuxiu Wu Fengqin Yin Jinsong Yu Liling Hao Fei Xu |
| author_sort | Nianxin Zhu |
| collection | DOAJ |
| description | Lead contamination in aquatic products is one of the main hazard factors. The aptasensor is a promising detection method for lead ion (Pb(II)) because of its selectivity, but it is easily affected by pH. The combination of ion-imprinted polymers(IIP) with aptamers may improve their stability in different pH conditions. This paper developed a novel electrochemical biosensor for Pb(II) detection by using aptamer-imprinted polymer as a recognition element. The glassy carbon electrode was modified with gold nanoparticles and aptamers. After the aptamer was induced by Pb(II) to form a G-quadruplex conformation, a chitosan-graphene oxide was electrodeposited and cross-linked with glutaraldehyde to form an imprint layer, improving the stability of the biosensor. Under the optimal experimental conditions, the current signal change (∆I) showed a linear correlation of the content of Pb(II) in the range of 0.1–2.0 μg/mL with a detection limit of 0.0796 μg/mL (S/N = 3). The biosensor also exhibited high selectivity for the determination of Pb(II) in the presence of other interfering metal ion. At the same time, the stability of the imprinted layer made the sensor applicable to the detection environment with a pH of 6.4–8.0. Moreover, the sensor was successfully applied to the detection of Pb(II) in mantis shrimp. |
| first_indexed | 2024-03-09T12:06:31Z |
| format | Article |
| id | doaj.art-3df4e3112f1e4145920a93b762a367dd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T12:06:31Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3df4e3112f1e4145920a93b762a367dd2023-11-30T22:57:08ZengMDPI AGMolecules1420-30492022-12-0128119610.3390/molecules28010196A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic ProductsNianxin Zhu0Xinna Liu1Kaimin Peng2Hui Cao3Min Yuan4Tai Ye5Xiuxiu Wu6Fengqin Yin7Jinsong Yu8Liling Hao9Fei Xu10Shanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaLead contamination in aquatic products is one of the main hazard factors. The aptasensor is a promising detection method for lead ion (Pb(II)) because of its selectivity, but it is easily affected by pH. The combination of ion-imprinted polymers(IIP) with aptamers may improve their stability in different pH conditions. This paper developed a novel electrochemical biosensor for Pb(II) detection by using aptamer-imprinted polymer as a recognition element. The glassy carbon electrode was modified with gold nanoparticles and aptamers. After the aptamer was induced by Pb(II) to form a G-quadruplex conformation, a chitosan-graphene oxide was electrodeposited and cross-linked with glutaraldehyde to form an imprint layer, improving the stability of the biosensor. Under the optimal experimental conditions, the current signal change (∆I) showed a linear correlation of the content of Pb(II) in the range of 0.1–2.0 μg/mL with a detection limit of 0.0796 μg/mL (S/N = 3). The biosensor also exhibited high selectivity for the determination of Pb(II) in the presence of other interfering metal ion. At the same time, the stability of the imprinted layer made the sensor applicable to the detection environment with a pH of 6.4–8.0. Moreover, the sensor was successfully applied to the detection of Pb(II) in mantis shrimp.https://www.mdpi.com/1420-3049/28/1/196aptamer-imprinted polymerchitosan–graphene oxide compositelead detection |
| spellingShingle | Nianxin Zhu Xinna Liu Kaimin Peng Hui Cao Min Yuan Tai Ye Xiuxiu Wu Fengqin Yin Jinsong Yu Liling Hao Fei Xu A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products Molecules aptamer-imprinted polymer chitosan–graphene oxide composite lead detection |
| title | A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products |
| title_full | A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products |
| title_fullStr | A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products |
| title_full_unstemmed | A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products |
| title_short | A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products |
| title_sort | novel aptamer imprinted polymer based electrochemical biosensor for the detection of lead in aquatic products |
| topic | aptamer-imprinted polymer chitosan–graphene oxide composite lead detection |
| url | https://www.mdpi.com/1420-3049/28/1/196 |
| work_keys_str_mv | AT nianxinzhu anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT xinnaliu anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT kaiminpeng anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT huicao anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT minyuan anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT taiye anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT xiuxiuwu anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT fengqinyin anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT jinsongyu anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT lilinghao anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT feixu anovelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT nianxinzhu novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT xinnaliu novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT kaiminpeng novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT huicao novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT minyuan novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT taiye novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT xiuxiuwu novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT fengqinyin novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT jinsongyu novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT lilinghao novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts AT feixu novelaptamerimprintedpolymerbasedelectrochemicalbiosensorforthedetectionofleadinaquaticproducts |