Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization
This study aimed to evaluate the interrelationship between telomere length, telomerase activity and oxidative DNA damage in patients undergoing in vitro fertilization (IVF). This single-center, observational clinical study comprised 102 unselected, consecutive patients with various infertility diagn...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/2/419 |
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| author | Sándor Péntek Ákos Várnagy Bálint Farkas Péter Mauchart Krisztina Gödöny Tímea Varjas Tamás Kőszegi Péter Kaltenecker Rita Jakabfi-Csepregi Kálmán Kovács József Bódis Endre Sulyok |
| author_facet | Sándor Péntek Ákos Várnagy Bálint Farkas Péter Mauchart Krisztina Gödöny Tímea Varjas Tamás Kőszegi Péter Kaltenecker Rita Jakabfi-Csepregi Kálmán Kovács József Bódis Endre Sulyok |
| author_sort | Sándor Péntek |
| collection | DOAJ |
| description | This study aimed to evaluate the interrelationship between telomere length, telomerase activity and oxidative DNA damage in patients undergoing in vitro fertilization (IVF). This single-center, observational clinical study comprised 102 unselected, consecutive patients with various infertility diagnoses. Granulosa cells (GCs) and follicular fluid (FF) were analyzed simultaneously for telomere functions and for the marker of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine (8-OHdG). An Absolute Human Telomere Lengths Quantification qPCR Assay kit and Telomerase Activity Quantification qPCR Assay kit (Nucleotestbio, Budapest, Hungary), as well as an 8-OHdG ELISA kit (Abbexa Ltd., Cambridge, United Kingdom) were used for analyses. Similar telomere lengths were found in GCs and FF, however telomerase activity was markedly depressed, while 8-OHdG levels were markedly elevated in FF compared with those in GCs (<i>p</i> < 0.01). Telomere lengths were independent of telomerase activity both in GCs and FF. However, GC 8-OHdG was inversely related to telomerase activity in GCs and FF (<i>p</i> < 0.05). Importantly, 8-OHdG levels both in GCs and FF had significant negative impact on the number of the retrieved and MII oocytes (<i>p</i> < 0.01), whereas FF 8-OHdG was negatively related further to the number of fertilized oocytes and blastocysts (<i>p</i> < 0.01). In conclusion, we could not confirm the direct association of telomere function and reproductive potential. However, oxidative DNA damage, as mainly reflected by 8-OHdG, adversely affected early markers of IVF outcome and clinical pregnancies. |
| first_indexed | 2024-03-11T09:13:52Z |
| format | Article |
| id | doaj.art-3dfb26888464474e84bfc5f023104094 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T09:13:52Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-3dfb26888464474e84bfc5f0231040942023-11-16T18:47:56ZengMDPI AGAntioxidants2076-39212023-02-0112241910.3390/antiox12020419Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro FertilizationSándor Péntek0Ákos Várnagy1Bálint Farkas2Péter Mauchart3Krisztina Gödöny4Tímea Varjas5Tamás Kőszegi6Péter Kaltenecker7Rita Jakabfi-Csepregi8Kálmán Kovács9József Bódis10Endre Sulyok11National Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryNational Laboratory on Human Reproduction, University of Pécs, 7622 Pécs, HungaryThis study aimed to evaluate the interrelationship between telomere length, telomerase activity and oxidative DNA damage in patients undergoing in vitro fertilization (IVF). This single-center, observational clinical study comprised 102 unselected, consecutive patients with various infertility diagnoses. Granulosa cells (GCs) and follicular fluid (FF) were analyzed simultaneously for telomere functions and for the marker of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine (8-OHdG). An Absolute Human Telomere Lengths Quantification qPCR Assay kit and Telomerase Activity Quantification qPCR Assay kit (Nucleotestbio, Budapest, Hungary), as well as an 8-OHdG ELISA kit (Abbexa Ltd., Cambridge, United Kingdom) were used for analyses. Similar telomere lengths were found in GCs and FF, however telomerase activity was markedly depressed, while 8-OHdG levels were markedly elevated in FF compared with those in GCs (<i>p</i> < 0.01). Telomere lengths were independent of telomerase activity both in GCs and FF. However, GC 8-OHdG was inversely related to telomerase activity in GCs and FF (<i>p</i> < 0.05). Importantly, 8-OHdG levels both in GCs and FF had significant negative impact on the number of the retrieved and MII oocytes (<i>p</i> < 0.01), whereas FF 8-OHdG was negatively related further to the number of fertilized oocytes and blastocysts (<i>p</i> < 0.01). In conclusion, we could not confirm the direct association of telomere function and reproductive potential. However, oxidative DNA damage, as mainly reflected by 8-OHdG, adversely affected early markers of IVF outcome and clinical pregnancies.https://www.mdpi.com/2076-3921/12/2/419telomere lengthtelomerase activityoxidative DNA damagein vitro fertilization |
| spellingShingle | Sándor Péntek Ákos Várnagy Bálint Farkas Péter Mauchart Krisztina Gödöny Tímea Varjas Tamás Kőszegi Péter Kaltenecker Rita Jakabfi-Csepregi Kálmán Kovács József Bódis Endre Sulyok Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization Antioxidants telomere length telomerase activity oxidative DNA damage in vitro fertilization |
| title | Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization |
| title_full | Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization |
| title_fullStr | Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization |
| title_full_unstemmed | Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization |
| title_short | Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization |
| title_sort | telomere length and telomerase activity of granulosa cells and follicular fluid in women undergoing in vitro fertilization |
| topic | telomere length telomerase activity oxidative DNA damage in vitro fertilization |
| url | https://www.mdpi.com/2076-3921/12/2/419 |
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