Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS)
Objective: Dual oxidases (DUOX1, DUOX2) belong to the NADPH oxidase (NOX) family, which produce H2O2 necessary for thyroid hormone biosynthesis. This study aims to evaluate gene expression for DUOX1, DUOX2 in PCOS patients and its relation with thyroid hormone and magnesium levels. Materials and me...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2023-12-01
|
| Series: | Journal of Family and Reproductive Health |
| Subjects: | |
| Online Access: | https://jfrh.tums.ac.ir/index.php/jfrh/article/view/2445 |
| _version_ | 1797359819474075648 |
|---|---|
| author | Ardeshir Hesampour Mina Jafarabadi Shima Rajabi Elahe Rezayof Akram Ghahghaei Nezamabadi |
| author_facet | Ardeshir Hesampour Mina Jafarabadi Shima Rajabi Elahe Rezayof Akram Ghahghaei Nezamabadi |
| author_sort | Ardeshir Hesampour |
| collection | DOAJ |
| description |
Objective: Dual oxidases (DUOX1, DUOX2) belong to the NADPH oxidase (NOX) family, which produce H2O2 necessary for thyroid hormone biosynthesis. This study aims to evaluate gene expression for DUOX1, DUOX2 in PCOS patients and its relation with thyroid hormone and magnesium levels.
Materials and methods: Totally 88 cases were studied including 24 people with PCOS and hypothyroidism, 44 people with PCOS and normal thyroid function, and 20 hypothyroid patients without PCOS. In comparison 40 healthy controls in the age group of 16-35 years matched for age group and BMI were evaluated. Using Vegaro syringe 5 cc of blood was sampled from all 128 people and after RNA extraction and cDNA synthesis using Real-Time PCR technique, the expression level of DUOX1 and DUOX2 genes was investigated.
Results: The results of hormonal tests showed that there is a significant difference between the level of T4, T3, and TSH hormones in hypothyroid patients with or without PCOS in comparison to the control group. Regarding the level of Mg, the results showed that there is a significant difference between the levels of Mg in PCOS group with or without hypothyroidism in comparison to the control group. Gene expression results showed that the relative changes of DUOX1 gene expression in different groups compared to the control group were significantly reduced P<0.05. In the polycystic group with hypothyroidism, the gene expression level showed a decrease compared to the normo-thyroid polycystic group and the hypothyroid non-PCO group, which was statistically significant P<0.05.
Conclusion: According to the results of the present study and the previous studies that have been published in the field of Duox1, it can be assumed that the reduction of Duox1 expression can interfere with the oxidative stress system. Further studies with other molecular techniques may help to understand the exact action mechanism of these genes.
|
| first_indexed | 2024-03-08T15:30:17Z |
| format | Article |
| id | doaj.art-3dfc376de9d94e449770b6d31f3d42ac |
| institution | Directory Open Access Journal |
| issn | 1735-8949 1735-9392 |
| language | English |
| last_indexed | 2024-03-08T15:30:17Z |
| publishDate | 2023-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Family and Reproductive Health |
| spelling | doaj.art-3dfc376de9d94e449770b6d31f3d42ac2024-01-10T06:12:20ZengTehran University of Medical SciencesJournal of Family and Reproductive Health1735-89491735-93922023-12-0117410.18502/jfrh.v17i4.14592Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS)Ardeshir Hesampour0Mina Jafarabadi1Shima Rajabi2Elahe Rezayof3Akram Ghahghaei Nezamabadi4Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, IranVali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Biology, Central Medical Sciences Unit, Islamic Azad University, Tehran, IranVali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Obstetrics and Gynecology, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran Objective: Dual oxidases (DUOX1, DUOX2) belong to the NADPH oxidase (NOX) family, which produce H2O2 necessary for thyroid hormone biosynthesis. This study aims to evaluate gene expression for DUOX1, DUOX2 in PCOS patients and its relation with thyroid hormone and magnesium levels. Materials and methods: Totally 88 cases were studied including 24 people with PCOS and hypothyroidism, 44 people with PCOS and normal thyroid function, and 20 hypothyroid patients without PCOS. In comparison 40 healthy controls in the age group of 16-35 years matched for age group and BMI were evaluated. Using Vegaro syringe 5 cc of blood was sampled from all 128 people and after RNA extraction and cDNA synthesis using Real-Time PCR technique, the expression level of DUOX1 and DUOX2 genes was investigated. Results: The results of hormonal tests showed that there is a significant difference between the level of T4, T3, and TSH hormones in hypothyroid patients with or without PCOS in comparison to the control group. Regarding the level of Mg, the results showed that there is a significant difference between the levels of Mg in PCOS group with or without hypothyroidism in comparison to the control group. Gene expression results showed that the relative changes of DUOX1 gene expression in different groups compared to the control group were significantly reduced P<0.05. In the polycystic group with hypothyroidism, the gene expression level showed a decrease compared to the normo-thyroid polycystic group and the hypothyroid non-PCO group, which was statistically significant P<0.05. Conclusion: According to the results of the present study and the previous studies that have been published in the field of Duox1, it can be assumed that the reduction of Duox1 expression can interfere with the oxidative stress system. Further studies with other molecular techniques may help to understand the exact action mechanism of these genes. https://jfrh.tums.ac.ir/index.php/jfrh/article/view/2445Dual Oxidase 1 (DUOX1) GeneDual Oxidase 2 (DUOX2) GenePolycystic Ovary SyndromeReal-Time Polymerase Chain Reaction (PCR) |
| spellingShingle | Ardeshir Hesampour Mina Jafarabadi Shima Rajabi Elahe Rezayof Akram Ghahghaei Nezamabadi Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS) Journal of Family and Reproductive Health Dual Oxidase 1 (DUOX1) Gene Dual Oxidase 2 (DUOX2) Gene Polycystic Ovary Syndrome Real-Time Polymerase Chain Reaction (PCR) |
| title | Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS) |
| title_full | Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS) |
| title_fullStr | Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS) |
| title_full_unstemmed | Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS) |
| title_short | Dual Oxidase 1, 2 Gene Expression in Women With Polycystic Ovary Syndrome (PCOS) |
| title_sort | dual oxidase 1 2 gene expression in women with polycystic ovary syndrome pcos |
| topic | Dual Oxidase 1 (DUOX1) Gene Dual Oxidase 2 (DUOX2) Gene Polycystic Ovary Syndrome Real-Time Polymerase Chain Reaction (PCR) |
| url | https://jfrh.tums.ac.ir/index.php/jfrh/article/view/2445 |
| work_keys_str_mv | AT ardeshirhesampour dualoxidase12geneexpressioninwomenwithpolycysticovarysyndromepcos AT minajafarabadi dualoxidase12geneexpressioninwomenwithpolycysticovarysyndromepcos AT shimarajabi dualoxidase12geneexpressioninwomenwithpolycysticovarysyndromepcos AT elaherezayof dualoxidase12geneexpressioninwomenwithpolycysticovarysyndromepcos AT akramghahghaeinezamabadi dualoxidase12geneexpressioninwomenwithpolycysticovarysyndromepcos |