Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production
The Green Revolution led to an increased use of synthetic pesticides, causing environmental pollution. As an alternative, biopesticides made from entomopathogenic agents such as fungi have been sought. This study aimed to design and evaluate the performance of a harvester machine for efficiently col...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | AgriEngineering |
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| Online Access: | https://www.mdpi.com/2624-7402/5/2/49 |
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| author | Fidel Diego-Nava Carlos Granados-Echegoyen Jaime Ruíz-Vega Teodulfo Aquino-Bolaños Rafael Pérez-Pacheco Alejo Díaz-Ramos Nancy Alonso-Hernández Fabián Arroyo-Balán Mónica Beatriz López-Hernández |
| author_facet | Fidel Diego-Nava Carlos Granados-Echegoyen Jaime Ruíz-Vega Teodulfo Aquino-Bolaños Rafael Pérez-Pacheco Alejo Díaz-Ramos Nancy Alonso-Hernández Fabián Arroyo-Balán Mónica Beatriz López-Hernández |
| author_sort | Fidel Diego-Nava |
| collection | DOAJ |
| description | The Green Revolution led to an increased use of synthetic pesticides, causing environmental pollution. As an alternative, biopesticides made from entomopathogenic agents such as fungi have been sought. This study aimed to design and evaluate the performance of a harvester machine for efficiently collecting entomopathogenic spores of <i>Metarhizium anisopliae</i> and <i>Beauveria bassiana</i> grown on rice and corn substrates. The spore yield was estimated, and a spore count and a colony-forming unit (CFU) count were performed. Statistical analysis was conducted to compare the mean values of spores obtained from different combinations of solid substrate and fungi. The Corn-<i>Metarhizium</i> combination produced 34.15 g of spores per kg of substrate and 1.51 × 10<sup>9</sup> CFUs mL<sup>−1</sup>. Similarly, the Rice-<i>Metarhizium</i> combination produced 57.35 g per kg and 1.59 × 10<sup>9</sup> CFUs mL<sup>−1</sup>. Meanwhile, the Corn-<i>Beauveria</i> combination yielded 35.47 g per kg and 1.00 × 10<sup>9</sup> CFUs mL<sup>−1</sup>, while the Rice-<i>Beauveria</i> combination had a yield of 38.26 g per kg and 4.50 × 10<sup>8</sup> CFUs mL<sup>−1</sup>. Based on the reported results, the Rice-<i>Metarhizium</i> combination appears to be the most effective, yielding the highest number of harvested spores per kg of substrate. The study estimated a total cost of approximately $409.31 for manufacturing the harvester, considering only the cost of the materials. These results could potentially increase the availability and affordability of entomopathogenic fungi in integrated pest management. |
| first_indexed | 2024-03-11T02:52:38Z |
| format | Article |
| id | doaj.art-3dff5975d7904373b2507f2294ede493 |
| institution | Directory Open Access Journal |
| issn | 2624-7402 |
| language | English |
| last_indexed | 2024-03-11T02:52:38Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | AgriEngineering |
| spelling | doaj.art-3dff5975d7904373b2507f2294ede4932023-11-18T08:52:49ZengMDPI AGAgriEngineering2624-74022023-04-015280181310.3390/agriengineering5020049Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide ProductionFidel Diego-Nava0Carlos Granados-Echegoyen1Jaime Ruíz-Vega2Teodulfo Aquino-Bolaños3Rafael Pérez-Pacheco4Alejo Díaz-Ramos5Nancy Alonso-Hernández6Fabián Arroyo-Balán7Mónica Beatriz López-Hernández8CIIDIR Unidad Oaxaca, Instituto Politécnico Nacional, Santa Cruz Xoxocotlán 71230, MexicoLaboratorio de Entomología Aplicada, Centro de Estudios en Desarrollo Sustentable y Aprovechamiento de la Vida Silvestre (CEDESU), CONACYT-Universidad Autónoma de Campeche, San Francisco de Campeche 24079, MexicoCIIDIR Unidad Oaxaca, Instituto Politécnico Nacional, Santa Cruz Xoxocotlán 71230, MexicoCIIDIR Unidad Oaxaca, Instituto Politécnico Nacional, Santa Cruz Xoxocotlán 71230, MexicoCIIDIR Unidad Oaxaca, Instituto Politécnico Nacional, Santa Cruz Xoxocotlán 71230, MexicoCIIDIR Unidad Oaxaca, Instituto Politécnico Nacional, Santa Cruz Xoxocotlán 71230, MexicoCIIDIR Unidad Oaxaca, Instituto Politécnico Nacional, Santa Cruz Xoxocotlán 71230, MexicoLaboratorio de Entomología Aplicada, Centro de Estudios en Desarrollo Sustentable y Aprovechamiento de la Vida Silvestre (CEDESU), CONACYT-Universidad Autónoma de Campeche, San Francisco de Campeche 24079, MexicoInstituto Tecnológico de Chiná (ITChiná), Tecnológico Nacional de Mexico, Chiná 24520, MexicoThe Green Revolution led to an increased use of synthetic pesticides, causing environmental pollution. As an alternative, biopesticides made from entomopathogenic agents such as fungi have been sought. This study aimed to design and evaluate the performance of a harvester machine for efficiently collecting entomopathogenic spores of <i>Metarhizium anisopliae</i> and <i>Beauveria bassiana</i> grown on rice and corn substrates. The spore yield was estimated, and a spore count and a colony-forming unit (CFU) count were performed. Statistical analysis was conducted to compare the mean values of spores obtained from different combinations of solid substrate and fungi. The Corn-<i>Metarhizium</i> combination produced 34.15 g of spores per kg of substrate and 1.51 × 10<sup>9</sup> CFUs mL<sup>−1</sup>. Similarly, the Rice-<i>Metarhizium</i> combination produced 57.35 g per kg and 1.59 × 10<sup>9</sup> CFUs mL<sup>−1</sup>. Meanwhile, the Corn-<i>Beauveria</i> combination yielded 35.47 g per kg and 1.00 × 10<sup>9</sup> CFUs mL<sup>−1</sup>, while the Rice-<i>Beauveria</i> combination had a yield of 38.26 g per kg and 4.50 × 10<sup>8</sup> CFUs mL<sup>−1</sup>. Based on the reported results, the Rice-<i>Metarhizium</i> combination appears to be the most effective, yielding the highest number of harvested spores per kg of substrate. The study estimated a total cost of approximately $409.31 for manufacturing the harvester, considering only the cost of the materials. These results could potentially increase the availability and affordability of entomopathogenic fungi in integrated pest management.https://www.mdpi.com/2624-7402/5/2/49biological controlbiopesticidesdesignentomopathogensharvester |
| spellingShingle | Fidel Diego-Nava Carlos Granados-Echegoyen Jaime Ruíz-Vega Teodulfo Aquino-Bolaños Rafael Pérez-Pacheco Alejo Díaz-Ramos Nancy Alonso-Hernández Fabián Arroyo-Balán Mónica Beatriz López-Hernández Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production AgriEngineering biological control biopesticides design entomopathogens harvester |
| title | Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production |
| title_full | Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production |
| title_fullStr | Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production |
| title_full_unstemmed | Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production |
| title_short | Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production |
| title_sort | functional and quality assessment of a spore harvester for entomopathogenic fungi for biopesticide production |
| topic | biological control biopesticides design entomopathogens harvester |
| url | https://www.mdpi.com/2624-7402/5/2/49 |
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