Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the dev...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2020.1825908 |
| _version_ | 1819103461165236224 |
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| author | Lucille F. van Beek Kristin Surmann H. Bart van den Berg van Saparoea Diane Houben Wouter S. P. Jong Christian Hentschker Thomas H. A. Ederveen Elena Mitsi Daniela M. Ferreira Fred van Opzeeland Christa E. van der Gaast – de Jongh Irma Joosten Uwe Völker Frank Schmidt Joen Luirink Dimitri A. Diavatopoulos Marien I. de Jonge |
| author_facet | Lucille F. van Beek Kristin Surmann H. Bart van den Berg van Saparoea Diane Houben Wouter S. P. Jong Christian Hentschker Thomas H. A. Ederveen Elena Mitsi Daniela M. Ferreira Fred van Opzeeland Christa E. van der Gaast – de Jongh Irma Joosten Uwe Völker Frank Schmidt Joen Luirink Dimitri A. Diavatopoulos Marien I. de Jonge |
| author_sort | Lucille F. van Beek |
| collection | DOAJ |
| description | Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of S. pneumoniae. Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen. |
| first_indexed | 2024-12-22T01:50:49Z |
| format | Article |
| id | doaj.art-3e016418b8e44a38bee691ecfa6505c3 |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-22T01:50:49Z |
| publishDate | 2020-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-3e016418b8e44a38bee691ecfa6505c32022-12-21T18:42:56ZengTaylor & Francis GroupVirulence2150-55942150-56082020-12-011111310132810.1080/21505594.2020.18259081825908Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigensLucille F. van Beek0Kristin Surmann1H. Bart van den Berg van Saparoea2Diane Houben3Wouter S. P. Jong4Christian Hentschker5Thomas H. A. Ederveen6Elena Mitsi7Daniela M. Ferreira8Fred van Opzeeland9Christa E. van der Gaast – de Jongh10Irma Joosten11Uwe Völker12Frank Schmidt13Joen Luirink14Dimitri A. Diavatopoulos15Marien I. de Jonge16Radboud Institute for Molecular Life SciencesUniversity Medicine GreifswaldAbera Bioscience ABAbera Bioscience ABAbera Bioscience ABUniversity Medicine GreifswaldRadboud University Medical CenterRespiratory Infection GroupRespiratory Infection GroupRadboud Institute for Molecular Life SciencesRadboud Institute for Molecular Life SciencesRadboud Institute for Molecular Life SciencesUniversity Medicine GreifswaldUniversity Medicine GreifswaldAbera Bioscience ABRadboud Institute for Molecular Life SciencesRadboud Institute for Molecular Life SciencesNasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of S. pneumoniae. Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen.http://dx.doi.org/10.1080/21505594.2020.1825908streptococcus pneumoniaetransition metalsnasal fluidprotein antigenscolonizationin vivo-mimicking |
| spellingShingle | Lucille F. van Beek Kristin Surmann H. Bart van den Berg van Saparoea Diane Houben Wouter S. P. Jong Christian Hentschker Thomas H. A. Ederveen Elena Mitsi Daniela M. Ferreira Fred van Opzeeland Christa E. van der Gaast – de Jongh Irma Joosten Uwe Völker Frank Schmidt Joen Luirink Dimitri A. Diavatopoulos Marien I. de Jonge Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens Virulence streptococcus pneumoniae transition metals nasal fluid protein antigens colonization in vivo-mimicking |
| title | Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens |
| title_full | Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens |
| title_fullStr | Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens |
| title_full_unstemmed | Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens |
| title_short | Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens |
| title_sort | exploring metal availability in the natural niche of streptococcus pneumoniae to discover potential vaccine antigens |
| topic | streptococcus pneumoniae transition metals nasal fluid protein antigens colonization in vivo-mimicking |
| url | http://dx.doi.org/10.1080/21505594.2020.1825908 |
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