The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer Patients
We studied the putative significance of angiotensin I-converting enzyme (ACE) in colorectal cancer (CRC) biology. Local expression of ACE was investigated by quantitative reverse transcription- polymerase chain reaction and by immunohistochemistry in CRCs and adenomas. ACE insertion (I)/deletion (D)...
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| Format: | Article |
| Language: | English |
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Elsevier
2007-09-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558607800315 |
| _version_ | 1819032041165946880 |
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| author | Christoph Röcken Konrad Neumann Stacy Carl-McGrath Hermann Lage Matthias P.A. Ebert Jutta Dierkes Christoph A. Jacobi Sinan Kalmuk Peter Neuhaus Ulf Neumann |
| author_facet | Christoph Röcken Konrad Neumann Stacy Carl-McGrath Hermann Lage Matthias P.A. Ebert Jutta Dierkes Christoph A. Jacobi Sinan Kalmuk Peter Neuhaus Ulf Neumann |
| author_sort | Christoph Röcken |
| collection | DOAJ |
| description | We studied the putative significance of angiotensin I-converting enzyme (ACE) in colorectal cancer (CRC) biology. Local expression of ACE was investigated by quantitative reverse transcription- polymerase chain reaction and by immunohistochemistry in CRCs and adenomas. ACE insertion (I)/deletion (D) polymorphism was studied in 141 CRC patients and 189 controls. ACE mRNA was upregulated in CRCs compared to corresponding nonlesional tissues (2.5-fold; P = .009). ACE protein was more commonly expressed in adenomas [17 (81 %)] and cancer epithelial cells [22 (100%)] than in corresponding non-neoplastic crypt and surface epithelium [2 (10%) and 2 (9%), respectively]. Thirty-seven CRC patients (26%) carried II genotype, 69 (49%) carried ID genotype, 35 (25%) carried DD genotype. The distribution of the genotypes did not differ from that of controls. Female CRC patients more commonly carried the ID genotype and less frequently the II and DD genotypes compared with male patients (P = .033). Men heterozygous or homozygous for the D-allele had larger tumors compared to carriers of the II genotype (P < .01). Women homozygous for the D-allele lived longer than carriers of the ID and II genotypes. Our study shows that ACE is differentially expressed in CRCs and that gene polymorphism is associated with gender-specific differences in primary tumor size and patient survival. |
| first_indexed | 2024-12-21T06:55:38Z |
| format | Article |
| id | doaj.art-3e05845e4f47471eae529e744d3febb3 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-21T06:55:38Z |
| publishDate | 2007-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-3e05845e4f47471eae529e744d3febb32022-12-21T19:12:20ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022007-09-019971672210.1593/neo.07418The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer PatientsChristoph Röcken0Konrad Neumann1Stacy Carl-McGrath2Hermann Lage3Matthias P.A. Ebert4Jutta Dierkes5Christoph A. Jacobi6Sinan Kalmuk7Peter Neuhaus8Ulf Neumann9Institute of Pathology, Charité University Hospital, Berlin, GermanyDepartment of Biometrics, Charité University Hospital, Berlin, GermanyInstitute of Pathology, Charité University Hospital, Berlin, GermanyInstitute of Pathology, Charité University Hospital, Berlin, GermanyDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität, Munchen, GermanyClinical Chemistry, Otto-von-Guericke University, Magdeburg, GermanyDepartment of General, Visceral, Vascular, Thoracic Surgery, Charité Campus Mitte, Charité University Hospital, Berlin, GermanyDepartment of General, Visceral, Transplantation Surgery, Charité Campus Virchow-Klinikum, Charité University Hospital, Berlin, GermanyDepartment of General, Visceral, Transplantation Surgery, Charité Campus Virchow-Klinikum, Charité University Hospital, Berlin, GermanyDepartment of General, Visceral, Transplantation Surgery, Charité Campus Virchow-Klinikum, Charité University Hospital, Berlin, GermanyWe studied the putative significance of angiotensin I-converting enzyme (ACE) in colorectal cancer (CRC) biology. Local expression of ACE was investigated by quantitative reverse transcription- polymerase chain reaction and by immunohistochemistry in CRCs and adenomas. ACE insertion (I)/deletion (D) polymorphism was studied in 141 CRC patients and 189 controls. ACE mRNA was upregulated in CRCs compared to corresponding nonlesional tissues (2.5-fold; P = .009). ACE protein was more commonly expressed in adenomas [17 (81 %)] and cancer epithelial cells [22 (100%)] than in corresponding non-neoplastic crypt and surface epithelium [2 (10%) and 2 (9%), respectively]. Thirty-seven CRC patients (26%) carried II genotype, 69 (49%) carried ID genotype, 35 (25%) carried DD genotype. The distribution of the genotypes did not differ from that of controls. Female CRC patients more commonly carried the ID genotype and less frequently the II and DD genotypes compared with male patients (P = .033). Men heterozygous or homozygous for the D-allele had larger tumors compared to carriers of the II genotype (P < .01). Women homozygous for the D-allele lived longer than carriers of the ID and II genotypes. Our study shows that ACE is differentially expressed in CRCs and that gene polymorphism is associated with gender-specific differences in primary tumor size and patient survival.http://www.sciencedirect.com/science/article/pii/S1476558607800315Colorectal cancerangiotensin-converting enzymepolymorphismgenderadenoma |
| spellingShingle | Christoph Röcken Konrad Neumann Stacy Carl-McGrath Hermann Lage Matthias P.A. Ebert Jutta Dierkes Christoph A. Jacobi Sinan Kalmuk Peter Neuhaus Ulf Neumann The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer Patients Neoplasia: An International Journal for Oncology Research Colorectal cancer angiotensin-converting enzyme polymorphism gender adenoma |
| title | The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer Patients |
| title_full | The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer Patients |
| title_fullStr | The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer Patients |
| title_full_unstemmed | The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer Patients |
| title_short | The Gene Polymorphism of the Angiotensin I-Converting Enzyme Correlates with Tumor Size and Patient Survival in Colorectal Cancer Patients |
| title_sort | gene polymorphism of the angiotensin i converting enzyme correlates with tumor size and patient survival in colorectal cancer patients |
| topic | Colorectal cancer angiotensin-converting enzyme polymorphism gender adenoma |
| url | http://www.sciencedirect.com/science/article/pii/S1476558607800315 |
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