Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner
We examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of Schistosoma japonicum and S. mansoni. Activity ascribable to aspartic protease(s) from S. japonicum and S. mansoni cleaved human hemoglobin. In ad...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2007-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000100014&lng=en&tlng=en |
| _version_ | 1797757174843179008 |
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| author | Jeffrey W Koehler Maria E Morales Bryan D Shelby Paul J Brindley |
| author_facet | Jeffrey W Koehler Maria E Morales Bryan D Shelby Paul J Brindley |
| author_sort | Jeffrey W Koehler |
| collection | DOAJ |
| description | We examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of Schistosoma japonicum and S. mansoni. Activity ascribable to aspartic protease(s) from S. japonicum and S. mansoni cleaved human hemoglobin. In addition, aspartic protease activities from S. japonicum cleaved hemoglobin from bovine, sheep, and horse blood more efficiently than did the activity from extracts of S. mansoni. These findings support the hypothesis that substrate specificity of hemoglobin-degrading proteases employed by blood feeding helminth parasites influences parasite host species range; differences in amino acid sequences in key sites of the parasite proteases interact less or more efficiently with the hemoglobins of permissive or non-permissive hosts. |
| first_indexed | 2024-03-12T18:12:10Z |
| format | Article |
| id | doaj.art-3e066ebdf8f34af9860088581a63edc5 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:12:10Z |
| publishDate | 2007-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-3e066ebdf8f34af9860088581a63edc52023-08-02T09:14:59ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602007-02-011021838510.1590/S0074-02762007000100014S0074-02762007000100014Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific mannerJeffrey W Koehler0Maria E Morales1Bryan D Shelby2Paul J Brindley3Tulane UniversityTulane UniversityTulane UniversityTulane UniversityWe examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of Schistosoma japonicum and S. mansoni. Activity ascribable to aspartic protease(s) from S. japonicum and S. mansoni cleaved human hemoglobin. In addition, aspartic protease activities from S. japonicum cleaved hemoglobin from bovine, sheep, and horse blood more efficiently than did the activity from extracts of S. mansoni. These findings support the hypothesis that substrate specificity of hemoglobin-degrading proteases employed by blood feeding helminth parasites influences parasite host species range; differences in amino acid sequences in key sites of the parasite proteases interact less or more efficiently with the hemoglobins of permissive or non-permissive hosts.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000100014&lng=en&tlng=enschistosomehemoglobinaspartic proteasecathepsin Dhost specificityhost range |
| spellingShingle | Jeffrey W Koehler Maria E Morales Bryan D Shelby Paul J Brindley Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner Memorias do Instituto Oswaldo Cruz schistosome hemoglobin aspartic protease cathepsin D host specificity host range |
| title | Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner |
| title_full | Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner |
| title_fullStr | Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner |
| title_full_unstemmed | Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner |
| title_short | Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner |
| title_sort | aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host specific manner |
| topic | schistosome hemoglobin aspartic protease cathepsin D host specificity host range |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000100014&lng=en&tlng=en |
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