Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic Approaches
(1) Background: The monkeypox virus is a zoonotic orthopox DNA virus that is closely linked to the virus. In light of the growing concern about this virus, the current research set out to use bioinformatics and immunoinformatics to develop a potential vaccine against the virus. (2) Methods: A multie...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/12/2010 |
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| author | Kunal Bhattacharya Israa M. Shamkh Mohammad Shahbaz Khan Marwa M. Lotfy Jean Bosco Nzeyimana Reem Fawaz Abutayeh Nadia M. Hamdy Dalia Hamza Nongmaithem Randhoni Chanu Pukar Khanal Atanu Bhattacharjee Emad B. Basalious |
| author_facet | Kunal Bhattacharya Israa M. Shamkh Mohammad Shahbaz Khan Marwa M. Lotfy Jean Bosco Nzeyimana Reem Fawaz Abutayeh Nadia M. Hamdy Dalia Hamza Nongmaithem Randhoni Chanu Pukar Khanal Atanu Bhattacharjee Emad B. Basalious |
| author_sort | Kunal Bhattacharya |
| collection | DOAJ |
| description | (1) Background: The monkeypox virus is a zoonotic orthopox DNA virus that is closely linked to the virus. In light of the growing concern about this virus, the current research set out to use bioinformatics and immunoinformatics to develop a potential vaccine against the virus. (2) Methods: A multiepitope vaccine was constructed from the B-cell and T-cell epitopes of the MPXVgp181 strain using adjuvant and different linkers. The constructed vaccine was predicted for antigenicity, allergenicity, toxicity, and population coverage. In silico immune simulation studies were also carried out. Expression analysis and cloning of the constructed vaccine was carried out in the pET-28a(+) vector using snapgene. (3) Results: The constructed vaccine was predicted to be antigenic, non-allergenic, and non-toxic. It was predicted to have excellent global population coverage and produced satisfactory immune response. The in silico expression and cloning studies were successful in <i>E. coli</i>, which makes the vaccine construct suitable for mass production in the pharmaceutical industry. (4) Conclusion: The constructed vaccine is based on the B-cell and T-cell epitopes obtained from the MPXVgp181 strain. This research can be useful in developing a vaccine to combat the monkeypox virus globally after performing in-depth in vitro and in vivo studies. |
| first_indexed | 2024-03-09T15:45:37Z |
| format | Article |
| id | doaj.art-3e0a4bf5dc904237b93d59850b9debfb |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T15:45:37Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-3e0a4bf5dc904237b93d59850b9debfb2023-11-24T18:31:07ZengMDPI AGVaccines2076-393X2022-11-011012201010.3390/vaccines10122010Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic ApproachesKunal Bhattacharya0Israa M. Shamkh1Mohammad Shahbaz Khan2Marwa M. Lotfy3Jean Bosco Nzeyimana4Reem Fawaz Abutayeh5Nadia M. Hamdy6Dalia Hamza7Nongmaithem Randhoni Chanu8Pukar Khanal9Atanu Bhattacharjee10Emad B. Basalious11Pratiksha Institute of Pharmaceutical Sciences, Guwahati 781026, Assam, IndiaBotany and Microbiology Department, Faculty of Science, Cairo University, Giza 12613, EgyptChildren’s National Hospital, Washington, DC 20010, USAFaculty of Pharmacy, Zagazig University, Zagazig 44511, EgyptCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Pharmaceutical Chemistry and Pharmacognosy, Applied Science Private University, Amman 11931, JordanBiochemistry Department, Faculty of Pharmacy, Ain Shams University, Abassia, Cairo 11566, EgyptZoonoses Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptFaculty of Pharmaceutical Science, Assam Downtown University, Guwahati 781026, Assam, IndiaDepartment of Pharmacology, NGSM Institute of Pharmaceutical Sciences, NITTE Deemed-to-be University, Mangalore 575018, Karnataka, IndiaRoyal School of Pharmacy, The Assam Royal Global University, Guwahati 781035, Assam, IndiaDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Al Kasr El-Aini, Cairo 11562, Egypt(1) Background: The monkeypox virus is a zoonotic orthopox DNA virus that is closely linked to the virus. In light of the growing concern about this virus, the current research set out to use bioinformatics and immunoinformatics to develop a potential vaccine against the virus. (2) Methods: A multiepitope vaccine was constructed from the B-cell and T-cell epitopes of the MPXVgp181 strain using adjuvant and different linkers. The constructed vaccine was predicted for antigenicity, allergenicity, toxicity, and population coverage. In silico immune simulation studies were also carried out. Expression analysis and cloning of the constructed vaccine was carried out in the pET-28a(+) vector using snapgene. (3) Results: The constructed vaccine was predicted to be antigenic, non-allergenic, and non-toxic. It was predicted to have excellent global population coverage and produced satisfactory immune response. The in silico expression and cloning studies were successful in <i>E. coli</i>, which makes the vaccine construct suitable for mass production in the pharmaceutical industry. (4) Conclusion: The constructed vaccine is based on the B-cell and T-cell epitopes obtained from the MPXVgp181 strain. This research can be useful in developing a vaccine to combat the monkeypox virus globally after performing in-depth in vitro and in vivo studies.https://www.mdpi.com/2076-393X/10/12/2010monkeypox virusmulti-epitope vaccinereverse vaccinologyimmunoinformaticsbioinformatics |
| spellingShingle | Kunal Bhattacharya Israa M. Shamkh Mohammad Shahbaz Khan Marwa M. Lotfy Jean Bosco Nzeyimana Reem Fawaz Abutayeh Nadia M. Hamdy Dalia Hamza Nongmaithem Randhoni Chanu Pukar Khanal Atanu Bhattacharjee Emad B. Basalious Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic Approaches Vaccines monkeypox virus multi-epitope vaccine reverse vaccinology immunoinformatics bioinformatics |
| title | Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic Approaches |
| title_full | Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic Approaches |
| title_fullStr | Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic Approaches |
| title_full_unstemmed | Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic Approaches |
| title_short | Multi-Epitope Vaccine Design against Monkeypox Virus via Reverse Vaccinology Method Exploiting Immunoinformatic and Bioinformatic Approaches |
| title_sort | multi epitope vaccine design against monkeypox virus via reverse vaccinology method exploiting immunoinformatic and bioinformatic approaches |
| topic | monkeypox virus multi-epitope vaccine reverse vaccinology immunoinformatics bioinformatics |
| url | https://www.mdpi.com/2076-393X/10/12/2010 |
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