Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i>
<i>Saururus chinensis</i> (Lour.) Baill. is a perennial herb and grows in Korea, China, and Japan. Interestingly, (7<i>S</i>,8<i>S</i>)-Δ<sup>8′</sup>-3,4-methylenedioxy-3′,5,5′-trimethoxy-7-monoacetate-8.<i>O</i>.4′-neolignan (MTMN), one o...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/8/932 |
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| author | Sae-Rom Yoo Hyekyung Ha Hyeun-Kyoo Shin Chang-Seob Seo |
| author_facet | Sae-Rom Yoo Hyekyung Ha Hyeun-Kyoo Shin Chang-Seob Seo |
| author_sort | Sae-Rom Yoo |
| collection | DOAJ |
| description | <i>Saururus chinensis</i> (Lour.) Baill. is a perennial herb and grows in Korea, China, and Japan. Interestingly, (7<i>S</i>,8<i>S</i>)-Δ<sup>8′</sup>-3,4-methylenedioxy-3′,5,5′-trimethoxy-7-monoacetate-8.<i>O</i>.4′-neolignan (MTMN), one of the active neolignans, was first isolated from the roots of <i>Saururus chinensis</i>. The compound was screened for anti-inflammatory activity using a RAW264.7 murine macrophage cell line. The dried roots of <i>S. chinensis</i> (9.7 kg) were extracted with 70% methanol and then solvent fractionation. From the ethyl acetate fraction, MTMN was purified through silica gel column and reverse-phase column chromatography and its structure was identified by spectroscopic analysis with nuclear magnetic resonance, circular dichroism, and mass spectrometry. RAW264.7 cells were induced using lipopolysaccharide (LPS) and treated with or without MTMN. Production of nitric oxide (NO) and prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) levels were measured and protein expressions of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were analyzed by immunoblotting. The isolated neolignan was (7S,8S)-Δ<sup>8′</sup>-3,4-methylenedioxy-3′,5,5′-trimethoxy-7-monoacetate-8.<i>O</i>.4′-neolignan. This compound suppressed the LPS-induced iNOS and COX-2 protein expressions, which led to a decrease in the production of NO and PGE<sub>2</sub> levels. Further studies, including in animal models, will be required to establish the precise pharmacological effect of MTMN. |
| first_indexed | 2024-03-10T18:15:46Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:15:46Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-3e0a7e85772e46f79daa49b10e4c2e7e2023-11-20T07:41:47ZengMDPI AGPlants2223-77472020-07-019893210.3390/plants9080932Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i>Sae-Rom Yoo0Hyekyung Ha1Hyeun-Kyoo Shin2Chang-Seob Seo3Korea Institute of Oriental Medicine, Daejeon 34054, KoreaKorea Institute of Oriental Medicine, Daejeon 34054, KoreaKorea Institute of Oriental Medicine, Daejeon 34054, KoreaKorea Institute of Oriental Medicine, Daejeon 34054, Korea<i>Saururus chinensis</i> (Lour.) Baill. is a perennial herb and grows in Korea, China, and Japan. Interestingly, (7<i>S</i>,8<i>S</i>)-Δ<sup>8′</sup>-3,4-methylenedioxy-3′,5,5′-trimethoxy-7-monoacetate-8.<i>O</i>.4′-neolignan (MTMN), one of the active neolignans, was first isolated from the roots of <i>Saururus chinensis</i>. The compound was screened for anti-inflammatory activity using a RAW264.7 murine macrophage cell line. The dried roots of <i>S. chinensis</i> (9.7 kg) were extracted with 70% methanol and then solvent fractionation. From the ethyl acetate fraction, MTMN was purified through silica gel column and reverse-phase column chromatography and its structure was identified by spectroscopic analysis with nuclear magnetic resonance, circular dichroism, and mass spectrometry. RAW264.7 cells were induced using lipopolysaccharide (LPS) and treated with or without MTMN. Production of nitric oxide (NO) and prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) levels were measured and protein expressions of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were analyzed by immunoblotting. The isolated neolignan was (7S,8S)-Δ<sup>8′</sup>-3,4-methylenedioxy-3′,5,5′-trimethoxy-7-monoacetate-8.<i>O</i>.4′-neolignan. This compound suppressed the LPS-induced iNOS and COX-2 protein expressions, which led to a decrease in the production of NO and PGE<sub>2</sub> levels. Further studies, including in animal models, will be required to establish the precise pharmacological effect of MTMN.https://www.mdpi.com/2223-7747/9/8/932<i>Saururus chinensis</i>neolignaninflammation |
| spellingShingle | Sae-Rom Yoo Hyekyung Ha Hyeun-Kyoo Shin Chang-Seob Seo Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i> Plants <i>Saururus chinensis</i> neolignan inflammation |
| title | Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i> |
| title_full | Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i> |
| title_fullStr | Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i> |
| title_full_unstemmed | Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i> |
| title_short | Anti-Inflamatory Activity of Neolignan Compound Isolated from the Roots of <i>Saururus chinensis</i> |
| title_sort | anti inflamatory activity of neolignan compound isolated from the roots of i saururus chinensis i |
| topic | <i>Saururus chinensis</i> neolignan inflammation |
| url | https://www.mdpi.com/2223-7747/9/8/932 |
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