Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces
In 2017, leaf stunting and yellowing were observed on twigs and branches of stone fruit trees in production regions in Razavi and North Khorasan provinces of Iran. For diagnosis of disease and identification of the causal agent, symptomatic leaf samples were collected in these orchards and were carr...
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2018-12-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_2212_d07ec33267d5a058668f6c892b2ba0e7.pdf |
| _version_ | 1818383262593056768 |
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| author | Saeed Tarighi Mojtaba Dehghan Ebadollah Ebadi |
| author_facet | Saeed Tarighi Mojtaba Dehghan Ebadollah Ebadi |
| author_sort | Saeed Tarighi |
| collection | DOAJ |
| description | In 2017, leaf stunting and yellowing were observed on twigs and branches of stone fruit trees in production regions in Razavi and North Khorasan provinces of Iran. For diagnosis of disease and identification of the causal agent, symptomatic leaf samples were collected in these orchards and were carried to the laboratory. The disease agent was successfully transmitted by grafting from naturally symptomatic samples to peach (Prunus persica) seedlings. Total DNA was extracted from plant samples and indexed by nested PCR using phytoplasma generic primers, P1/P7 and R16F2n/R2. PCR products were sequenced and the nucleotide sequences were compared with those of other phytoplasmas in GenBank and analyzed by virtual restriction fragment length polymorphism (RFLP). The phytoplasmas associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces were identified as members of the 16SrIX group or Pigeon pea witches broom (Candidatus Phytoplasma phoenicium). This is the first report of leaf stunting and yellowing disease and characterization of associated phytoplasma in these regions. |
| first_indexed | 2024-12-14T03:03:34Z |
| format | Article |
| id | doaj.art-3e0b46cf57f047b8ae3576d9454d3fa3 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-14T03:03:34Z |
| publishDate | 2018-12-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-3e0b46cf57f047b8ae3576d9454d3fa32022-12-21T23:19:27ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002018-12-0110312413110.22103/jab.2018.22122212Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provincesSaeed Tarighi0Mojtaba Dehghan1Ebadollah Ebadi2Dept. Plant Protection, Faculty of Agriculture, Ferdowsi University of MashhadPh.D. Student of plant pathology, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.Ph.D. Student of plant pathology, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.In 2017, leaf stunting and yellowing were observed on twigs and branches of stone fruit trees in production regions in Razavi and North Khorasan provinces of Iran. For diagnosis of disease and identification of the causal agent, symptomatic leaf samples were collected in these orchards and were carried to the laboratory. The disease agent was successfully transmitted by grafting from naturally symptomatic samples to peach (Prunus persica) seedlings. Total DNA was extracted from plant samples and indexed by nested PCR using phytoplasma generic primers, P1/P7 and R16F2n/R2. PCR products were sequenced and the nucleotide sequences were compared with those of other phytoplasmas in GenBank and analyzed by virtual restriction fragment length polymorphism (RFLP). The phytoplasmas associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces were identified as members of the 16SrIX group or Pigeon pea witches broom (Candidatus Phytoplasma phoenicium). This is the first report of leaf stunting and yellowing disease and characterization of associated phytoplasma in these regions.https://jab.uk.ac.ir/article_2212_d07ec33267d5a058668f6c892b2ba0e7.pdfphytoplasmamolecular analysis16srix group |
| spellingShingle | Saeed Tarighi Mojtaba Dehghan Ebadollah Ebadi Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces مجله بیوتکنولوژی کشاورزی phytoplasma molecular analysis 16srix group |
| title | Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces |
| title_full | Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces |
| title_fullStr | Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces |
| title_full_unstemmed | Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces |
| title_short | Identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in Razavi and North Khorasan provinces |
| title_sort | identification and molecular characterization of the phytoplasma associated with leaf stunting and yellowing disease in razavi and north khorasan provinces |
| topic | phytoplasma molecular analysis 16srix group |
| url | https://jab.uk.ac.ir/article_2212_d07ec33267d5a058668f6c892b2ba0e7.pdf |
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