Multispacer Sequence Typing for Mycobacterium bovis Genotyping
The molecular typing of Mycobacterium bovis, which causes bovine tuberculosis, can be accomplished by combining different polymorphic markers, contributing to its epidemiological investigation. Multispacer sequence typing (MST) is a sequencing-based method that employs intergenic regions susceptible...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-04-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.666283/full |
| _version_ | 1818961376599605248 |
|---|---|
| author | Érica Bravo Sales Antônio Augusto Fonseca Cristina Magalhães Gonçalves Andrey Pereira Lage Giovanna Ivo Andrade Philip Noel Suffys Harrison Magdinier Gomes Natanael Lamas Dias José Soares Ferreira Neto Ana Marcia de Sá Guimarães Marcos Bryan Heinemann |
| author_facet | Érica Bravo Sales Antônio Augusto Fonseca Cristina Magalhães Gonçalves Andrey Pereira Lage Giovanna Ivo Andrade Philip Noel Suffys Harrison Magdinier Gomes Natanael Lamas Dias José Soares Ferreira Neto Ana Marcia de Sá Guimarães Marcos Bryan Heinemann |
| author_sort | Érica Bravo Sales |
| collection | DOAJ |
| description | The molecular typing of Mycobacterium bovis, which causes bovine tuberculosis, can be accomplished by combining different polymorphic markers, contributing to its epidemiological investigation. Multispacer sequence typing (MST) is a sequencing-based method that employs intergenic regions susceptible to higher mutation rates given the low selection pressure. It has been applied to M. tuberculosis, but not to M. bovis. The aim of this study was to evaluate a MST for M. bovis. A total of 58 strains isolated from tissues with lesions suggestive of bovine tuberculosis, coming from cattle herds in six Brazilian states and four standard samples of M. bovis were typified employing the MST technique. Fourteen intergenic regions were used, and four types of genetic events were reported: single nucleotide mutation (SNP), insertion, deletion, and tandem repeat (TR). Seven loci were chosen for typing. Twenty-eight type sequences (ST) were identified, indicating type sequences (ST) were identified, indicating a 92.9% HGDI (Hunter Gaston Discriminatory Index). The data were used to analyze the evolutionary patterns of these isolates and correlate them to phylogeographic lineages based on the formation of clonal complexes generated from eBURST software. Later, we associated the MST with spoligotyping technique, currently considered the gold standard for classification of M. bovis. The results support the MST as an alternative method for genotyping of M. bovis. The method has the advantage of sequencing and the availability of sequences analyzed in public databases, which can be used by professionals around the world as a tool for further analysis. This was the first study to identify the variability of isolates of M. bovis by the MST method. |
| first_indexed | 2024-12-20T12:12:27Z |
| format | Article |
| id | doaj.art-3e0c7ba857c942e8980e26e599fc21d5 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-20T12:12:27Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-3e0c7ba857c942e8980e26e599fc21d52022-12-21T19:41:13ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-04-01810.3389/fvets.2021.666283666283Multispacer Sequence Typing for Mycobacterium bovis GenotypingÉrica Bravo Sales0Antônio Augusto Fonseca1Cristina Magalhães Gonçalves2Andrey Pereira Lage3Giovanna Ivo Andrade4Philip Noel Suffys5Harrison Magdinier Gomes6Natanael Lamas Dias7José Soares Ferreira Neto8Ana Marcia de Sá Guimarães9Marcos Bryan Heinemann10Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Minas Gerais, BrazilLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Minas Gerais, BrazilLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Minas Gerais, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, BrazilFioCruz, Rio de Janeiro, Rio de Janeiro, BrazilFioCruz, Rio de Janeiro, Rio de Janeiro, BrazilLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Minas Gerais, BrazilUniversidade de São Paulo, São Paulo, São Paulo, BrazilUniversidade de São Paulo, São Paulo, São Paulo, BrazilUniversidade de São Paulo, São Paulo, São Paulo, BrazilThe molecular typing of Mycobacterium bovis, which causes bovine tuberculosis, can be accomplished by combining different polymorphic markers, contributing to its epidemiological investigation. Multispacer sequence typing (MST) is a sequencing-based method that employs intergenic regions susceptible to higher mutation rates given the low selection pressure. It has been applied to M. tuberculosis, but not to M. bovis. The aim of this study was to evaluate a MST for M. bovis. A total of 58 strains isolated from tissues with lesions suggestive of bovine tuberculosis, coming from cattle herds in six Brazilian states and four standard samples of M. bovis were typified employing the MST technique. Fourteen intergenic regions were used, and four types of genetic events were reported: single nucleotide mutation (SNP), insertion, deletion, and tandem repeat (TR). Seven loci were chosen for typing. Twenty-eight type sequences (ST) were identified, indicating type sequences (ST) were identified, indicating a 92.9% HGDI (Hunter Gaston Discriminatory Index). The data were used to analyze the evolutionary patterns of these isolates and correlate them to phylogeographic lineages based on the formation of clonal complexes generated from eBURST software. Later, we associated the MST with spoligotyping technique, currently considered the gold standard for classification of M. bovis. The results support the MST as an alternative method for genotyping of M. bovis. The method has the advantage of sequencing and the availability of sequences analyzed in public databases, which can be used by professionals around the world as a tool for further analysis. This was the first study to identify the variability of isolates of M. bovis by the MST method.https://www.frontiersin.org/articles/10.3389/fvets.2021.666283/fullMycobactereium bovisbovine tuberculosisgenotypeepidemiologyMST |
| spellingShingle | Érica Bravo Sales Antônio Augusto Fonseca Cristina Magalhães Gonçalves Andrey Pereira Lage Giovanna Ivo Andrade Philip Noel Suffys Harrison Magdinier Gomes Natanael Lamas Dias José Soares Ferreira Neto Ana Marcia de Sá Guimarães Marcos Bryan Heinemann Multispacer Sequence Typing for Mycobacterium bovis Genotyping Frontiers in Veterinary Science Mycobactereium bovis bovine tuberculosis genotype epidemiology MST |
| title | Multispacer Sequence Typing for Mycobacterium bovis Genotyping |
| title_full | Multispacer Sequence Typing for Mycobacterium bovis Genotyping |
| title_fullStr | Multispacer Sequence Typing for Mycobacterium bovis Genotyping |
| title_full_unstemmed | Multispacer Sequence Typing for Mycobacterium bovis Genotyping |
| title_short | Multispacer Sequence Typing for Mycobacterium bovis Genotyping |
| title_sort | multispacer sequence typing for mycobacterium bovis genotyping |
| topic | Mycobactereium bovis bovine tuberculosis genotype epidemiology MST |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.666283/full |
| work_keys_str_mv | AT ericabravosales multispacersequencetypingformycobacteriumbovisgenotyping AT antonioaugustofonseca multispacersequencetypingformycobacteriumbovisgenotyping AT cristinamagalhaesgoncalves multispacersequencetypingformycobacteriumbovisgenotyping AT andreypereiralage multispacersequencetypingformycobacteriumbovisgenotyping AT giovannaivoandrade multispacersequencetypingformycobacteriumbovisgenotyping AT philipnoelsuffys multispacersequencetypingformycobacteriumbovisgenotyping AT harrisonmagdiniergomes multispacersequencetypingformycobacteriumbovisgenotyping AT natanaellamasdias multispacersequencetypingformycobacteriumbovisgenotyping AT josesoaresferreiraneto multispacersequencetypingformycobacteriumbovisgenotyping AT anamarciadesaguimaraes multispacersequencetypingformycobacteriumbovisgenotyping AT marcosbryanheinemann multispacersequencetypingformycobacteriumbovisgenotyping |