Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy Fields
Paddy fields account for 10% of global CH<sub>4</sub> emissions, and the application of manure may increase CH<sub>4</sub> emissions. In this study, high-throughput sequencing technology was used to investigate the effects of manure application on CH<sub>4</sub> e...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2073-445X/11/4/499 |
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| author | Jianqiang Wu Min Wang Peng Li Leyang Shen Mingyi Ma Boyu Xu Shuyuan Zhang Chenyan Sha Chunmei Ye Lijun Xiong Shenfa Huang |
| author_facet | Jianqiang Wu Min Wang Peng Li Leyang Shen Mingyi Ma Boyu Xu Shuyuan Zhang Chenyan Sha Chunmei Ye Lijun Xiong Shenfa Huang |
| author_sort | Jianqiang Wu |
| collection | DOAJ |
| description | Paddy fields account for 10% of global CH<sub>4</sub> emissions, and the application of manure may increase CH<sub>4</sub> emissions. In this study, high-throughput sequencing technology was used to investigate the effects of manure application on CH<sub>4</sub> emissions and methanogens in paddy soil. Three treatments were studied: a controlled treatment (CK), pig manure (PM), and organic fertilizer (OF). The results showed that the contents of Zn, Cr and Ni in paddy soil increased with the application of manure, but the contents of heavy metals gradually decreased with the growth of rice. The Shannon index and Ace index showed that the application of pig manure and organic fertilizer less affected the diversity and richness of soil Archaea. The results of community composition analysis showed that <i>Methanobacterium</i>, <i>Methanobrevibacter</i>, <i>Methanosphaera</i>, <i>Methanosarcina</i> and <i>Rice_Cluster_I</i> were the main methanogens in paddy soil after manure and organic fertilizer application. Soil environmental factors were changed after applied manure, among which total potassium (TK) and total nitrogen (TN) were the main environmental factors affecting methanogens in paddy soil. The changes of soil environmental factors affected the community composition of methanogens, and the increase of the relative abundance of methanogens maybe the main reason for the increase of CH<sub>4</sub> emission flux. The relative abundance of methanogens and CH<sub>4</sub> emission flux in paddy soil were increased by both pig manure and organic fertilizer application, and pig manure had a bigger impact than organic manure. |
| first_indexed | 2024-03-09T10:32:52Z |
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| last_indexed | 2024-03-09T10:32:52Z |
| publishDate | 2022-03-01 |
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| spelling | doaj.art-3e10871360d14d999143ec55b30b3f162023-12-01T21:09:25ZengMDPI AGLand2073-445X2022-03-0111449910.3390/land11040499Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy FieldsJianqiang Wu0Min Wang1Peng Li2Leyang Shen3Mingyi Ma4Boyu Xu5Shuyuan Zhang6Chenyan Sha7Chunmei Ye8Lijun Xiong9Shenfa Huang10Shanghai Academy of Environmental Sciences, Shanghai 200233, ChinaShanghai Academy of Environmental Sciences, Shanghai 200233, ChinaCollege of Environmental Science and Engineering, Donghua University, Shanghai 201600, ChinaShanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power, Shanghai University of Electric Power, Shanghai 200090, ChinaShanghai Science and Technology Museum Station, Shanghai 200231, ChinaShanghai Academy of Environmental Sciences, Shanghai 200233, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaShanghai Academy of Environmental Sciences, Shanghai 200233, ChinaShanghai Academy of Environmental Sciences, Shanghai 200233, ChinaShanghai Academy of Environmental Sciences, Shanghai 200233, ChinaShanghai Academy of Environmental Sciences, Shanghai 200233, ChinaPaddy fields account for 10% of global CH<sub>4</sub> emissions, and the application of manure may increase CH<sub>4</sub> emissions. In this study, high-throughput sequencing technology was used to investigate the effects of manure application on CH<sub>4</sub> emissions and methanogens in paddy soil. Three treatments were studied: a controlled treatment (CK), pig manure (PM), and organic fertilizer (OF). The results showed that the contents of Zn, Cr and Ni in paddy soil increased with the application of manure, but the contents of heavy metals gradually decreased with the growth of rice. The Shannon index and Ace index showed that the application of pig manure and organic fertilizer less affected the diversity and richness of soil Archaea. The results of community composition analysis showed that <i>Methanobacterium</i>, <i>Methanobrevibacter</i>, <i>Methanosphaera</i>, <i>Methanosarcina</i> and <i>Rice_Cluster_I</i> were the main methanogens in paddy soil after manure and organic fertilizer application. Soil environmental factors were changed after applied manure, among which total potassium (TK) and total nitrogen (TN) were the main environmental factors affecting methanogens in paddy soil. The changes of soil environmental factors affected the community composition of methanogens, and the increase of the relative abundance of methanogens maybe the main reason for the increase of CH<sub>4</sub> emission flux. The relative abundance of methanogens and CH<sub>4</sub> emission flux in paddy soil were increased by both pig manure and organic fertilizer application, and pig manure had a bigger impact than organic manure.https://www.mdpi.com/2073-445X/11/4/499pig manureorganic fertilizerpaddy soilCH<sub>4</sub>methanogens |
| spellingShingle | Jianqiang Wu Min Wang Peng Li Leyang Shen Mingyi Ma Boyu Xu Shuyuan Zhang Chenyan Sha Chunmei Ye Lijun Xiong Shenfa Huang Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy Fields Land pig manure organic fertilizer paddy soil CH<sub>4</sub> methanogens |
| title | Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy Fields |
| title_full | Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy Fields |
| title_fullStr | Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy Fields |
| title_full_unstemmed | Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy Fields |
| title_short | Effects of Pig Manure and Its Organic Fertilizer Application on Archaea and Methane Emission in Paddy Fields |
| title_sort | effects of pig manure and its organic fertilizer application on archaea and methane emission in paddy fields |
| topic | pig manure organic fertilizer paddy soil CH<sub>4</sub> methanogens |
| url | https://www.mdpi.com/2073-445X/11/4/499 |
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