Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis
A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present stu...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Lavras
2013-08-01
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| Series: | Ciência e Agrotecnologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008&lng=en&tlng=en |
| _version_ | 1828811011638951936 |
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| author | Vinícius Paula Venâncio Eric Batista Ferreira Maísa Ribeiro Pereira Lima Brigagão Fernanda Borges de Araújo Paula Luis Fernando Barbisan Luciana Azevedo |
| author_facet | Vinícius Paula Venâncio Eric Batista Ferreira Maísa Ribeiro Pereira Lima Brigagão Fernanda Borges de Araújo Paula Luis Fernando Barbisan Luciana Azevedo |
| author_sort | Vinícius Paula Venâncio |
| collection | DOAJ |
| description | A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats. |
| first_indexed | 2024-12-12T09:20:27Z |
| format | Article |
| id | doaj.art-3e110daeb556437f82c06d3f1a749123 |
| institution | Directory Open Access Journal |
| issn | 1981-1829 |
| language | English |
| last_indexed | 2024-12-12T09:20:27Z |
| publishDate | 2013-08-01 |
| publisher | Universidade Federal de Lavras |
| record_format | Article |
| series | Ciência e Agrotecnologia |
| spelling | doaj.art-3e110daeb556437f82c06d3f1a7491232022-12-22T00:29:14ZengUniversidade Federal de LavrasCiência e Agrotecnologia1981-18292013-08-0137434334910.1590/S1413-70542013000400008S1413-70542013000400008Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesisVinícius Paula Venâncio0Eric Batista Ferreira1Maísa Ribeiro Pereira Lima Brigagão2Fernanda Borges de Araújo Paula3Luis Fernando Barbisan4Luciana Azevedo5Universidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Estadual PaulistaUniversidade Estadual PaulistaA. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008&lng=en&tlng=enFocos de cripta aberrantescerrado brasileirodimetilhidrazinaimunohistoquímicaestresse oxidativo |
| spellingShingle | Vinícius Paula Venâncio Eric Batista Ferreira Maísa Ribeiro Pereira Lima Brigagão Fernanda Borges de Araújo Paula Luis Fernando Barbisan Luciana Azevedo Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis Ciência e Agrotecnologia Focos de cripta aberrantes cerrado brasileiro dimetilhidrazina imunohistoquímica estresse oxidativo |
| title | Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
| title_full | Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
| title_fullStr | Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
| title_full_unstemmed | Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
| title_short | Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
| title_sort | annona crassiflora mart fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
| topic | Focos de cripta aberrantes cerrado brasileiro dimetilhidrazina imunohistoquímica estresse oxidativo |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008&lng=en&tlng=en |
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