The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships
Escherichia coli Direct Oxygen Sensor (Ec DOS, also known as Ec DosP) is a heme-based O2-sensing phosphodiesterase from Escherichia coli that catalyzes the conversion of cyclic-di-GMP to linear di-GMP. Cyclic-di-GMP is an important second messenger in bacteria, highlighting the importance of underst...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-06-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-6374/3/2/211 |
| _version_ | 1811308815571746816 |
|---|---|
| author | Toru Shimizu |
| author_facet | Toru Shimizu |
| author_sort | Toru Shimizu |
| collection | DOAJ |
| description | Escherichia coli Direct Oxygen Sensor (Ec DOS, also known as Ec DosP) is a heme-based O2-sensing phosphodiesterase from Escherichia coli that catalyzes the conversion of cyclic-di-GMP to linear di-GMP. Cyclic-di-GMP is an important second messenger in bacteria, highlighting the importance of understanding structure-function relationships of Ec DOS. Ec DOS is composed of an N-terminal heme-bound O2-sensing PAS domain and a C-terminal phosphodiesterase catalytic domain. Notably, its activity is markedly enhanced by O2 binding to the heme Fe(II) complex in the PAS sensor domain. X-ray crystal structures and spectroscopic and catalytic characterization of the wild-type and mutant proteins have provided important structural and functional clues to understanding the molecular mechanism of intramolecular catalytic regulation by O2 binding. This review summarizes the intriguing findings that have obtained for Ec DOS. |
| first_indexed | 2024-04-13T09:29:54Z |
| format | Article |
| id | doaj.art-3e13991000d7464bbc22122544cdc639 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-04-13T09:29:54Z |
| publishDate | 2013-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-3e13991000d7464bbc22122544cdc6392022-12-22T02:52:16ZengMDPI AGBiosensors2079-63742013-06-013221123710.3390/bios3020211The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function RelationshipsToru ShimizuEscherichia coli Direct Oxygen Sensor (Ec DOS, also known as Ec DosP) is a heme-based O2-sensing phosphodiesterase from Escherichia coli that catalyzes the conversion of cyclic-di-GMP to linear di-GMP. Cyclic-di-GMP is an important second messenger in bacteria, highlighting the importance of understanding structure-function relationships of Ec DOS. Ec DOS is composed of an N-terminal heme-bound O2-sensing PAS domain and a C-terminal phosphodiesterase catalytic domain. Notably, its activity is markedly enhanced by O2 binding to the heme Fe(II) complex in the PAS sensor domain. X-ray crystal structures and spectroscopic and catalytic characterization of the wild-type and mutant proteins have provided important structural and functional clues to understanding the molecular mechanism of intramolecular catalytic regulation by O2 binding. This review summarizes the intriguing findings that have obtained for Ec DOS.http://www.mdpi.com/2079-6374/3/2/211heme proteinoxygen sensorphosphodiesterasec-AMPc-di-GMPsignal transduction |
| spellingShingle | Toru Shimizu The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships Biosensors heme protein oxygen sensor phosphodiesterase c-AMP c-di-GMP signal transduction |
| title | The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships |
| title_full | The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships |
| title_fullStr | The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships |
| title_full_unstemmed | The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships |
| title_short | The Heme-Based Oxygen-Sensor Phosphodiesterase Ec DOS (DosP): Structure-Function Relationships |
| title_sort | heme based oxygen sensor phosphodiesterase ec dos dosp structure function relationships |
| topic | heme protein oxygen sensor phosphodiesterase c-AMP c-di-GMP signal transduction |
| url | http://www.mdpi.com/2079-6374/3/2/211 |
| work_keys_str_mv | AT torushimizu thehemebasedoxygensensorphosphodiesteraseecdosdospstructurefunctionrelationships AT torushimizu hemebasedoxygensensorphosphodiesteraseecdosdospstructurefunctionrelationships |