Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i>
The transposition of insertion sequence elements was evaluated among different <i>Deinococcus geothermalis</i> lineages, including the wild-type, a cystine importer-disrupted mutant, a complemented strain, and a cystine importer-overexpressed strain. Cellular growth reached early exponen...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/10/1623 |
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| author | Qianying Ye Chanjae Lee Eunjung Shin Sung-Jae Lee |
| author_facet | Qianying Ye Chanjae Lee Eunjung Shin Sung-Jae Lee |
| author_sort | Qianying Ye |
| collection | DOAJ |
| description | The transposition of insertion sequence elements was evaluated among different <i>Deinococcus geothermalis</i> lineages, including the wild-type, a cystine importer-disrupted mutant, a complemented strain, and a cystine importer-overexpressed strain. Cellular growth reached early exponential growth at OD<sub>600</sub> 2.0 and late exponential growth at OD<sub>600</sub> 4.0. Exposing the cells to hydrogen peroxide (80–100 mM) resulted in the transposition of insertion sequences (ISs) in genes associated with the carotenoid biosynthesis pathway. Particularly, IS<i>Dge7</i> (an IS<i>5</i> family member) and IS<i>Dge5</i> (an IS<i>701</i> family member) from the cystine importer-disrupted mutant were transposed into phytoene desaturase (<i>dgeo</i>_0524) via replicative transposition. Further, the cystine importer-overexpressed strain Δ<i>dgeo</i>_1985R showed transposition of both IS<i>Dge2</i> and IS<i>Dge5</i> elements. In contrast, IS transposition was not detected in the complementary strain. Interestingly, a cystine importer-overexpressing strain exhibited streptomycin resistance, indicating that point mutation occurred in the <i>rpsL</i> (<i>dgeo</i>_1873) gene encoding ribosomal protein S12. qRT-PCR analyses were then conducted to evaluate the expression of oxidative stress response genes, IS elements, and low-molecular-weight thiol compounds such as mycothiol and bacillithiol. Nevertheless, the mechanisms that trigger IS transposition in redox imbalance conditions remain unclear. Here, we report that the active transposition of different IS elements was affected by intracellular redox imbalances caused by cystine importer deficiencies or overexpression. |
| first_indexed | 2024-03-10T06:45:11Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T06:45:11Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-3e149e765e054d93b2a80faf9109e6372023-11-22T17:16:54ZengMDPI AGAntioxidants2076-39212021-10-011010162310.3390/antiox10101623Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i>Qianying Ye0Chanjae Lee1Eunjung Shin2Sung-Jae Lee3Department of Biology, Kyung Hee University, Seoul 02447, KoreaDepartment of Biology, Kyung Hee University, Seoul 02447, KoreaDepartment of Biology, Kyung Hee University, Seoul 02447, KoreaDepartment of Biology, Kyung Hee University, Seoul 02447, KoreaThe transposition of insertion sequence elements was evaluated among different <i>Deinococcus geothermalis</i> lineages, including the wild-type, a cystine importer-disrupted mutant, a complemented strain, and a cystine importer-overexpressed strain. Cellular growth reached early exponential growth at OD<sub>600</sub> 2.0 and late exponential growth at OD<sub>600</sub> 4.0. Exposing the cells to hydrogen peroxide (80–100 mM) resulted in the transposition of insertion sequences (ISs) in genes associated with the carotenoid biosynthesis pathway. Particularly, IS<i>Dge7</i> (an IS<i>5</i> family member) and IS<i>Dge5</i> (an IS<i>701</i> family member) from the cystine importer-disrupted mutant were transposed into phytoene desaturase (<i>dgeo</i>_0524) via replicative transposition. Further, the cystine importer-overexpressed strain Δ<i>dgeo</i>_1985R showed transposition of both IS<i>Dge2</i> and IS<i>Dge5</i> elements. In contrast, IS transposition was not detected in the complementary strain. Interestingly, a cystine importer-overexpressing strain exhibited streptomycin resistance, indicating that point mutation occurred in the <i>rpsL</i> (<i>dgeo</i>_1873) gene encoding ribosomal protein S12. qRT-PCR analyses were then conducted to evaluate the expression of oxidative stress response genes, IS elements, and low-molecular-weight thiol compounds such as mycothiol and bacillithiol. Nevertheless, the mechanisms that trigger IS transposition in redox imbalance conditions remain unclear. Here, we report that the active transposition of different IS elements was affected by intracellular redox imbalances caused by cystine importer deficiencies or overexpression.https://www.mdpi.com/2076-3921/10/10/1623<i>Deinococcus geothermalis</i>cystine importerinsertion sequencesoxidative stressredox-balancetranscriptomic analysis |
| spellingShingle | Qianying Ye Chanjae Lee Eunjung Shin Sung-Jae Lee Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i> Antioxidants <i>Deinococcus geothermalis</i> cystine importer insertion sequences oxidative stress redox-balance transcriptomic analysis |
| title | Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i> |
| title_full | Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i> |
| title_fullStr | Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i> |
| title_full_unstemmed | Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i> |
| title_short | Influence of Redox Imbalances on the Transposition of Insertion Sequences in <i>Deinococcus geothermalis</i> |
| title_sort | influence of redox imbalances on the transposition of insertion sequences in i deinococcus geothermalis i |
| topic | <i>Deinococcus geothermalis</i> cystine importer insertion sequences oxidative stress redox-balance transcriptomic analysis |
| url | https://www.mdpi.com/2076-3921/10/10/1623 |
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