Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathway
Objective To observe the effects and mechanisms of Apelin-13 on expression of pyroptosis related proteins in aorta of diabetic mice. Methods C57/BL mice of eight weeks old were used as control group;kkAy mice of eight weeks old were used as type 2 diabetic models;osmotic pumps were used to treat kkA...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a181092.pdf |
| _version_ | 1797365708783353856 |
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| author | WANG Yang-jia, ZHANG Jia, LI Bin, ZENG Xiang-jun |
| author_facet | WANG Yang-jia, ZHANG Jia, LI Bin, ZENG Xiang-jun |
| author_sort | WANG Yang-jia, ZHANG Jia, LI Bin, ZENG Xiang-jun |
| collection | DOAJ |
| description | Objective To observe the effects and mechanisms of Apelin-13 on expression of pyroptosis related proteins in aorta of diabetic mice. Methods C57/BL mice of eight weeks old were used as control group;kkAy mice of eight weeks old were used as type 2 diabetic models;osmotic pumps were used to treat kkAy mice with apelin-13 at a rate of 30 μg/(kg·d),and L-NAME(eNOS inhibitor) was injected intraperitoneally at a dose of 10 mg/(kg·d) to kkAy mice. Blood was collected for detection of Hb1Ac. The aortae were harvested and fixed. Morphological changes were observed with HE staining. Expression of eNOS,NLRP3,caspase-1 and gasdermin D were measured with immunohistochemical staining. Results Compared to the control group, the level of eNOS in the aorta of diabetic mice was significantly higher than that in control mice (P＜0.05),the levels of NLRP3,caspase-1 and gasdermin D were also higher than that in control mice. After apelin-13 treatment,the expressions of eNOS,NLRP3,caspase-1 and gasdermin D were further increased (P＜0.05). After L-NAME and apelin-13 treatment, the expressions of eNOS, NLRP3,caspase-1 and gasdermin D were reduced as compared to apelin-13 treatment alone(P＜0.05). Conclusions Apelin-13 may promote the expression of pyroptosis related protein in aortic cells by increasing eNOS/NO pathway,which would induce structural and functional damage in diabetic arteries. |
| first_indexed | 2024-03-08T16:52:48Z |
| format | Article |
| id | doaj.art-3e1553edddc94bda9c067ca76280c5de |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:52:48Z |
| publishDate | 2020-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-3e1553edddc94bda9c067ca76280c5de2024-01-05T03:14:04ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-02-01402155160Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathwayWANG Yang-jia, ZHANG Jia, LI Bin, ZENG Xiang-jun0Department of Physiology and Pathophysiology, Capital Medical University, Beijing 100069, ChinaObjective To observe the effects and mechanisms of Apelin-13 on expression of pyroptosis related proteins in aorta of diabetic mice. Methods C57/BL mice of eight weeks old were used as control group;kkAy mice of eight weeks old were used as type 2 diabetic models;osmotic pumps were used to treat kkAy mice with apelin-13 at a rate of 30 μg/(kg·d),and L-NAME(eNOS inhibitor) was injected intraperitoneally at a dose of 10 mg/(kg·d) to kkAy mice. Blood was collected for detection of Hb1Ac. The aortae were harvested and fixed. Morphological changes were observed with HE staining. Expression of eNOS,NLRP3,caspase-1 and gasdermin D were measured with immunohistochemical staining. Results Compared to the control group, the level of eNOS in the aorta of diabetic mice was significantly higher than that in control mice (P＜0.05),the levels of NLRP3,caspase-1 and gasdermin D were also higher than that in control mice. After apelin-13 treatment,the expressions of eNOS,NLRP3,caspase-1 and gasdermin D were further increased (P＜0.05). After L-NAME and apelin-13 treatment, the expressions of eNOS, NLRP3,caspase-1 and gasdermin D were reduced as compared to apelin-13 treatment alone(P＜0.05). Conclusions Apelin-13 may promote the expression of pyroptosis related protein in aortic cells by increasing eNOS/NO pathway,which would induce structural and functional damage in diabetic arteries.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a181092.pdfdiabetes|aorta|apelin-13|pyroptosis|enos |
| spellingShingle | WANG Yang-jia, ZHANG Jia, LI Bin, ZENG Xiang-jun Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathway Jichu yixue yu linchuang diabetes|aorta|apelin-13|pyroptosis|enos |
| title | Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathway |
| title_full | Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathway |
| title_fullStr | Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathway |
| title_full_unstemmed | Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathway |
| title_short | Apelin-13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through eNOS/NO pathway |
| title_sort | apelin 13 promotes expression of pyroptosis related protein in aortic cells in diabetic mice through enos no pathway |
| topic | diabetes|aorta|apelin-13|pyroptosis|enos |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a181092.pdf |
| work_keys_str_mv | AT wangyangjiazhangjialibinzengxiangjun apelin13promotesexpressionofpyroptosisrelatedproteininaorticcellsindiabeticmicethroughenosnopathway |