3-D worm tracker for freely moving C. elegans.
The manner in which the nervous system regulates animal behaviors in natural environments is a fundamental issue in biology. To address this question, C. elegans has been widely used as a model animal for the analysis of various animal behaviors. Previous behavioral assays have been limited to two-d...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3578814?pdf=render |
| _version_ | 1818970743271063552 |
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| author | Namseop Kwon Jaeyeon Pyo Seung-Jae Lee Jung Ho Je |
| author_facet | Namseop Kwon Jaeyeon Pyo Seung-Jae Lee Jung Ho Je |
| author_sort | Namseop Kwon |
| collection | DOAJ |
| description | The manner in which the nervous system regulates animal behaviors in natural environments is a fundamental issue in biology. To address this question, C. elegans has been widely used as a model animal for the analysis of various animal behaviors. Previous behavioral assays have been limited to two-dimensional (2-D) environments, confining the worm motion to a planar substrate that does not reflect three-dimensional (3-D) natural environments such as rotting fruits or soil. Here, we develop a 3-D worm tracker (3DWT) for freely moving C. elegans in 3-D environments, based on a stereoscopic configuration. The 3DWT provides us with a quantitative trajectory, including the position and movement direction of the worm in 3-D. The 3DWT is also capable of recording and visualizing postures of the moving worm in 3-D, which are more complex than those in 2-D. Our 3DWT affords new opportunities for understanding the nervous system function that regulates animal behaviors in natural 3-D environments. |
| first_indexed | 2024-12-20T14:41:20Z |
| format | Article |
| id | doaj.art-3e1580408125407980a35a389fa49930 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T14:41:20Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3e1580408125407980a35a389fa499302022-12-21T19:37:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5748410.1371/journal.pone.00574843-D worm tracker for freely moving C. elegans.Namseop KwonJaeyeon PyoSeung-Jae LeeJung Ho JeThe manner in which the nervous system regulates animal behaviors in natural environments is a fundamental issue in biology. To address this question, C. elegans has been widely used as a model animal for the analysis of various animal behaviors. Previous behavioral assays have been limited to two-dimensional (2-D) environments, confining the worm motion to a planar substrate that does not reflect three-dimensional (3-D) natural environments such as rotting fruits or soil. Here, we develop a 3-D worm tracker (3DWT) for freely moving C. elegans in 3-D environments, based on a stereoscopic configuration. The 3DWT provides us with a quantitative trajectory, including the position and movement direction of the worm in 3-D. The 3DWT is also capable of recording and visualizing postures of the moving worm in 3-D, which are more complex than those in 2-D. Our 3DWT affords new opportunities for understanding the nervous system function that regulates animal behaviors in natural 3-D environments.http://europepmc.org/articles/PMC3578814?pdf=render |
| spellingShingle | Namseop Kwon Jaeyeon Pyo Seung-Jae Lee Jung Ho Je 3-D worm tracker for freely moving C. elegans. PLoS ONE |
| title | 3-D worm tracker for freely moving C. elegans. |
| title_full | 3-D worm tracker for freely moving C. elegans. |
| title_fullStr | 3-D worm tracker for freely moving C. elegans. |
| title_full_unstemmed | 3-D worm tracker for freely moving C. elegans. |
| title_short | 3-D worm tracker for freely moving C. elegans. |
| title_sort | 3 d worm tracker for freely moving c elegans |
| url | http://europepmc.org/articles/PMC3578814?pdf=render |
| work_keys_str_mv | AT namseopkwon 3dwormtrackerforfreelymovingcelegans AT jaeyeonpyo 3dwormtrackerforfreelymovingcelegans AT seungjaelee 3dwormtrackerforfreelymovingcelegans AT junghoje 3dwormtrackerforfreelymovingcelegans |