Protocol for generation and assessment of head-like structure in zebrafish
Summary: In vitro embryonic analogue models, such as gastruloids, trunk-like structures and embryoids, have been developed to understand principles of early development and morphogenesis. However, models that can fully mimic head formation are still missing. Here, we present a protocol for generatin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723005208 |
| _version_ | 1797679416631885824 |
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| author | Tao Cheng Yan-Yi Xing Yang Dong Peng-Fei Xu |
| author_facet | Tao Cheng Yan-Yi Xing Yang Dong Peng-Fei Xu |
| author_sort | Tao Cheng |
| collection | DOAJ |
| description | Summary: In vitro embryonic analogue models, such as gastruloids, trunk-like structures and embryoids, have been developed to understand principles of early development and morphogenesis. However, models that can fully mimic head formation are still missing. Here, we present a protocol for generating the head-like structure (HLS) in zebrafish embryonic explants. We describe steps for dissection and constructing cell and patterning landscapes. We then detail assessment of this structure through axis induction.For complete details on the use and execution of this protocol, please refer to Cheng et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-11T23:14:18Z |
| format | Article |
| id | doaj.art-3e16e6f573a341cba3f8c8ab07969ecd |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-11T23:14:18Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-3e16e6f573a341cba3f8c8ab07969ecd2023-09-21T04:38:09ZengElsevierSTAR Protocols2666-16672023-12-0144102553Protocol for generation and assessment of head-like structure in zebrafishTao Cheng0Yan-Yi Xing1Yang Dong2Peng-Fei Xu3Women’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaWomen’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Laboratory of Genetic & Developmental Disorders, Hangzhou, Zhejiang, ChinaWomen’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaWomen’s Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Corresponding authorSummary: In vitro embryonic analogue models, such as gastruloids, trunk-like structures and embryoids, have been developed to understand principles of early development and morphogenesis. However, models that can fully mimic head formation are still missing. Here, we present a protocol for generating the head-like structure (HLS) in zebrafish embryonic explants. We describe steps for dissection and constructing cell and patterning landscapes. We then detail assessment of this structure through axis induction.For complete details on the use and execution of this protocol, please refer to Cheng et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723005208Developmental BiologyRNAseqMicroscopyStem Cells |
| spellingShingle | Tao Cheng Yan-Yi Xing Yang Dong Peng-Fei Xu Protocol for generation and assessment of head-like structure in zebrafish STAR Protocols Developmental Biology RNAseq Microscopy Stem Cells |
| title | Protocol for generation and assessment of head-like structure in zebrafish |
| title_full | Protocol for generation and assessment of head-like structure in zebrafish |
| title_fullStr | Protocol for generation and assessment of head-like structure in zebrafish |
| title_full_unstemmed | Protocol for generation and assessment of head-like structure in zebrafish |
| title_short | Protocol for generation and assessment of head-like structure in zebrafish |
| title_sort | protocol for generation and assessment of head like structure in zebrafish |
| topic | Developmental Biology RNAseq Microscopy Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723005208 |
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