Antiproliferative Activity of Fucan Nanogel
Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated l-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecyla...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-09-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/10/9/2002 |
| _version_ | 1811304413987340288 |
|---|---|
| author | Francisco Miguel Gama Hugo Alexandre Oliveira Rocha Paula Pereira Silvia Santos Pedrosa Arthur Anthunes Jacome Vidal Ruth Medeiros Oliveira Dayanne Lopes Gomes Jailma Almeida-Lima Nednaldo Dantas-Santos |
| author_facet | Francisco Miguel Gama Hugo Alexandre Oliveira Rocha Paula Pereira Silvia Santos Pedrosa Arthur Anthunes Jacome Vidal Ruth Medeiros Oliveira Dayanne Lopes Gomes Jailma Almeida-Lima Nednaldo Dantas-Santos |
| author_sort | Francisco Miguel Gama |
| collection | DOAJ |
| description | Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated l-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of −38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%–43.7% at nanogel concentrations of 0.05–0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%–22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle. |
| first_indexed | 2024-04-13T08:06:41Z |
| format | Article |
| id | doaj.art-3e18fe559aa24ca28ba8ae077b49644f |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T08:06:41Z |
| publishDate | 2012-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3e18fe559aa24ca28ba8ae077b49644f2022-12-22T02:55:08ZengMDPI AGMarine Drugs1660-33972012-09-011092002202210.3390/md10092002Antiproliferative Activity of Fucan NanogelFrancisco Miguel GamaHugo Alexandre Oliveira RochaPaula PereiraSilvia Santos PedrosaArthur Anthunes Jacome VidalRuth Medeiros OliveiraDayanne Lopes GomesJailma Almeida-LimaNednaldo Dantas-SantosSulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated l-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of −38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%–43.7% at nanogel concentrations of 0.05–0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%–22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.http://www.mdpi.com/1660-3397/10/9/2002nanogelscancer cellssulfated polysaccharidesSpatoglossum schröederi |
| spellingShingle | Francisco Miguel Gama Hugo Alexandre Oliveira Rocha Paula Pereira Silvia Santos Pedrosa Arthur Anthunes Jacome Vidal Ruth Medeiros Oliveira Dayanne Lopes Gomes Jailma Almeida-Lima Nednaldo Dantas-Santos Antiproliferative Activity of Fucan Nanogel Marine Drugs nanogels cancer cells sulfated polysaccharides Spatoglossum schröederi |
| title | Antiproliferative Activity of Fucan Nanogel |
| title_full | Antiproliferative Activity of Fucan Nanogel |
| title_fullStr | Antiproliferative Activity of Fucan Nanogel |
| title_full_unstemmed | Antiproliferative Activity of Fucan Nanogel |
| title_short | Antiproliferative Activity of Fucan Nanogel |
| title_sort | antiproliferative activity of fucan nanogel |
| topic | nanogels cancer cells sulfated polysaccharides Spatoglossum schröederi |
| url | http://www.mdpi.com/1660-3397/10/9/2002 |
| work_keys_str_mv | AT franciscomiguelgama antiproliferativeactivityoffucannanogel AT hugoalexandreoliveirarocha antiproliferativeactivityoffucannanogel AT paulapereira antiproliferativeactivityoffucannanogel AT silviasantospedrosa antiproliferativeactivityoffucannanogel AT arthuranthunesjacomevidal antiproliferativeactivityoffucannanogel AT ruthmedeirosoliveira antiproliferativeactivityoffucannanogel AT dayannelopesgomes antiproliferativeactivityoffucannanogel AT jailmaalmeidalima antiproliferativeactivityoffucannanogel AT nednaldodantassantos antiproliferativeactivityoffucannanogel |