Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methods
Babesiosis, a very important vector disease, has also been detected in humans and animals. This study was carried out on 120 whole blood samples taken from Cankiri Municipality Animal Care and Rehabilitation Center (Türkiye) between December 2021 and 2022 in Cankiri. In this study, venous blood samp...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3052 |
| _version_ | 1797356192106807296 |
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| author | Ozcan OZKAN Banucicek YUCESAN Yusuf YILMAZ Zekeriya OCAL |
| author_facet | Ozcan OZKAN Banucicek YUCESAN Yusuf YILMAZ Zekeriya OCAL |
| author_sort | Ozcan OZKAN |
| collection | DOAJ |
| description | Babesiosis, a very important vector disease, has also been detected in humans and
animals. This study was carried out on 120 whole blood samples taken from Cankiri
Municipality Animal Care and Rehabilitation Center (Türkiye) between December
2021 and 2022 in Cankiri. In this study, venous blood samples taken by a veterinarian
were used for diagnosis. The 18S rRNA gene region was examined with conventional
PCR analysis and the positive results were subjected to sequence analysis. As a result
of sequence analysis, these positive cases were determined to be Babesia bigemina.
Additionally, as a result of statistical analysis, it was found that dogs detected positive
for B. bigemina did not give significant results according to age, gender or symptoms.
B. bigemina detected in this study were registered in the NCBI database with 7 (Acc no:
OQ186727), 12 (Acc no: OQ186720), 19 (Acc no: OQ186721), 21 (Acc no: OQ186722),
23 (Acc no: OQ18723), 28 (Acc no: OQ186724), 30 (Acc no: OQ186725), and 70 (Acc
no: OQ186726) numbers. The B. bigemina species detected in dogs in this study is
actually seen in farm animals. This study is the first detection report of B. bigemina in
dogs in Turkey. As a result, Babesia species seen in dogs need to be reconsidered and
investigated with the development of molecular techniques. |
| first_indexed | 2024-03-08T14:23:01Z |
| format | Article |
| id | doaj.art-3e198ffe74a542d9b7119a3061bd2bf4 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-08T14:23:01Z |
| publishDate | 2023-11-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-3e198ffe74a542d9b7119a3061bd2bf42024-01-13T17:59:32ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-11-01301414510.9775/kvfd.2023.303563052Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methodsOzcan OZKAN0Banucicek YUCESAN1Yusuf YILMAZ2Zekeriya OCALCankiri Karatekin University, Faculty of Science, Biology Department, TR-18200 Çankırı - TÜRKİYECankiri Karatekin University, Faculty of Health Science, Control of Zoonotic Health Diseases Department, TR-18200 Çankırı - TÜRKİYERepublic of Türkiye, Ministry of Health, Public Health General Directorate of Türkiye, Microbiology Reference Laboratories and Biological Products Department, TR-06430 Ankara - TÜRKİYEBabesiosis, a very important vector disease, has also been detected in humans and animals. This study was carried out on 120 whole blood samples taken from Cankiri Municipality Animal Care and Rehabilitation Center (Türkiye) between December 2021 and 2022 in Cankiri. In this study, venous blood samples taken by a veterinarian were used for diagnosis. The 18S rRNA gene region was examined with conventional PCR analysis and the positive results were subjected to sequence analysis. As a result of sequence analysis, these positive cases were determined to be Babesia bigemina. Additionally, as a result of statistical analysis, it was found that dogs detected positive for B. bigemina did not give significant results according to age, gender or symptoms. B. bigemina detected in this study were registered in the NCBI database with 7 (Acc no: OQ186727), 12 (Acc no: OQ186720), 19 (Acc no: OQ186721), 21 (Acc no: OQ186722), 23 (Acc no: OQ18723), 28 (Acc no: OQ186724), 30 (Acc no: OQ186725), and 70 (Acc no: OQ186726) numbers. The B. bigemina species detected in dogs in this study is actually seen in farm animals. This study is the first detection report of B. bigemina in dogs in Turkey. As a result, Babesia species seen in dogs need to be reconsidered and investigated with the development of molecular techniques.https://vetdergikafkas.org/pdf.php?id=3052babesiababesia bigeminababesiosisdog |
| spellingShingle | Ozcan OZKAN Banucicek YUCESAN Yusuf YILMAZ Zekeriya OCAL Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methods Kafkas Universitesi Veteriner Fakültesi Dergisi babesia babesia bigemina babesiosis dog |
| title | Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methods |
| title_full | Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methods |
| title_fullStr | Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methods |
| title_full_unstemmed | Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methods |
| title_short | Detection of babesia bigemina cases in dogs in rural areas/türkiye by molecular methods |
| title_sort | detection of babesia bigemina cases in dogs in rural areas turkiye by molecular methods |
| topic | babesia babesia bigemina babesiosis dog |
| url | https://vetdergikafkas.org/pdf.php?id=3052 |
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