Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA
Campylobacter jejuni is a predominant zoonotic pathogen causing gastroenteritis and other diseases in humans. An important bacterial virulence factor is the secreted serine protease HtrA (HtrACj), which targets tight and adherens junctional proteins in the gut epithelium. Here we have investigated t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-11-01
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| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19490976.2020.1810532 |
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| author | Urszula Zarzecka Alessandro Grinzato Eaazhisai Kandiah Dominik Cysewski Paola Berto Joanna Skorko-Glonek Giuseppe Zanotti Steffen Backert |
| author_facet | Urszula Zarzecka Alessandro Grinzato Eaazhisai Kandiah Dominik Cysewski Paola Berto Joanna Skorko-Glonek Giuseppe Zanotti Steffen Backert |
| author_sort | Urszula Zarzecka |
| collection | DOAJ |
| description | Campylobacter jejuni is a predominant zoonotic pathogen causing gastroenteritis and other diseases in humans. An important bacterial virulence factor is the secreted serine protease HtrA (HtrACj), which targets tight and adherens junctional proteins in the gut epithelium. Here we have investigated the function and structure of HtrACj using biochemical assays and cryo-electron microscopy. Mass spectrometry analysis identified differences and similarities in the cleavage site specificity for HtrACj by comparison to the HtrA counterparts from Helicobacter pylori and Escherichia coli. We defined the architecture of HtrACj at 5.8 Å resolution as a dodecamer, built of four trimers. The contacts between the trimers are quite loose, a fact that explains the flexibility and mobility of the dodecameric assembly. This flexibility has also been studied through molecular dynamics simulation, which revealed opening of the dodecamer to expose the proteolytically active site of the protease. Moreover, we examined the rearrangements at the level of oligomerization in the presence or absence of substrate using size exclusion chromatography, which revealed hexamers, dodecamers and larger oligomeric forms, as well as remarkable stability of higher oligomeric forms (> 12-mers) compared to previously tested homologs from other bacteria. Extremely dynamic decay of the higher oligomeric forms into lower forms was observed after full cleavage of the substrate by the proteolytically active variant of HtrACj. Together, this is the first report on the in-depth functional and structural analysis of HtrACj, which may allow the construction of therapeutically relevant HtrACj inhibitors in the near future. |
| first_indexed | 2024-12-13T14:46:36Z |
| format | Article |
| id | doaj.art-3e1c73b9202d40c5a7e6e195b7a3c111 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-12-13T14:46:36Z |
| publishDate | 2020-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-3e1c73b9202d40c5a7e6e195b7a3c1112022-12-21T23:41:27ZengTaylor & Francis GroupGut Microbes1949-09761949-09842020-11-0112110.1080/19490976.2020.18105321810532Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrAUrszula Zarzecka0Alessandro Grinzato1Eaazhisai Kandiah2Dominik Cysewski3Paola Berto4Joanna Skorko-Glonek5Giuseppe Zanotti6Steffen Backert7Friedrich-Alexander-University Erlangen-NurembergUniversity of PaduaEuropean Synchrotron Radiation Facility (ESRF)Institute of Biochemistry and Biophysics, Polish Academy of ScienceUniversity of PaduaUniversity of GdańskUniversity of PaduaFriedrich-Alexander-University Erlangen-NurembergCampylobacter jejuni is a predominant zoonotic pathogen causing gastroenteritis and other diseases in humans. An important bacterial virulence factor is the secreted serine protease HtrA (HtrACj), which targets tight and adherens junctional proteins in the gut epithelium. Here we have investigated the function and structure of HtrACj using biochemical assays and cryo-electron microscopy. Mass spectrometry analysis identified differences and similarities in the cleavage site specificity for HtrACj by comparison to the HtrA counterparts from Helicobacter pylori and Escherichia coli. We defined the architecture of HtrACj at 5.8 Å resolution as a dodecamer, built of four trimers. The contacts between the trimers are quite loose, a fact that explains the flexibility and mobility of the dodecameric assembly. This flexibility has also been studied through molecular dynamics simulation, which revealed opening of the dodecamer to expose the proteolytically active site of the protease. Moreover, we examined the rearrangements at the level of oligomerization in the presence or absence of substrate using size exclusion chromatography, which revealed hexamers, dodecamers and larger oligomeric forms, as well as remarkable stability of higher oligomeric forms (> 12-mers) compared to previously tested homologs from other bacteria. Extremely dynamic decay of the higher oligomeric forms into lower forms was observed after full cleavage of the substrate by the proteolytically active variant of HtrACj. Together, this is the first report on the in-depth functional and structural analysis of HtrACj, which may allow the construction of therapeutically relevant HtrACj inhibitors in the near future.http://dx.doi.org/10.1080/19490976.2020.1810532campylobacter jejunihtraproteaseoligomerizationcryo-emcleavage site specificitythermal stability |
| spellingShingle | Urszula Zarzecka Alessandro Grinzato Eaazhisai Kandiah Dominik Cysewski Paola Berto Joanna Skorko-Glonek Giuseppe Zanotti Steffen Backert Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA Gut Microbes campylobacter jejuni htra protease oligomerization cryo-em cleavage site specificity thermal stability |
| title | Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA |
| title_full | Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA |
| title_fullStr | Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA |
| title_full_unstemmed | Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA |
| title_short | Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA |
| title_sort | functional analysis and cryo electron microscopy of campylobacter jejuni serine protease htra |
| topic | campylobacter jejuni htra protease oligomerization cryo-em cleavage site specificity thermal stability |
| url | http://dx.doi.org/10.1080/19490976.2020.1810532 |
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