Analysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomas
Background/aim: Among the genes involved in ovarian carcinogenesis, there has been increased interest in tumor-suppressor genes p53 and BRCA1. Both of the genes make control of cell cycle, DNA repair and apoptosis. The p53 is a "genome guardian" inactivated in more than 50% of human cancer...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Military Health Department, Ministry of Defence, Serbia
2006-01-01
|
| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2006/0042-84500609813P.pdf |
| _version_ | 1826574825208938496 |
|---|---|
| author | Luković Ljiljana Popović Branka Atanacković Jasmina Novaković Ivana Perović Milica Petrović Bojana Petković Spasoje |
| author_facet | Luković Ljiljana Popović Branka Atanacković Jasmina Novaković Ivana Perović Milica Petrović Bojana Petković Spasoje |
| author_sort | Luković Ljiljana |
| collection | DOAJ |
| description | Background/aim: Among the genes involved in ovarian carcinogenesis, there has been increased interest in tumor-suppressor genes p53 and BRCA1. Both of the genes make control of cell cycle, DNA repair and apoptosis. The p53 is a "genome guardian" inactivated in more than 50% of human cancers, while BRCA1 mutations are found mostly in breast and ovarian cancer. The aim of this investigation was to establish the frequency of loss of heterozygosity (LOH) in the regions of the genes p53 and BRCA1 in ovarian carcinomas, and to analyze the association of LOH with the disease stage and prognosis. Methods. We analyzed 20 patients with a confirmed diagnosis of epithelilal ovarian carcinoma. DNA for molecular-genetic analysis was extracted from the tumor tissue and blood as normal tissue of each person. Microsatellite markers of the regions of genes p53 and BRCA1 were amplified by PCR method. The determination of allelic status of microsatellites and detection of LOH was performed after PAA gel electroforesis. Results. Both of the analyzed microsatellite markers were informative in 13/20 (65%) cases. In the region of gene p53, LOH was established in 4/13 (30.7%) tumors. One of them had histological gradus G1, one had gradus G2, and two of them had gradus G3, while all were with the International Federation of Gynecology and Obstetrics (FIGO) IIIc stage. In the region of gene BRCA1, LOH was detected in 5/13 (38.5%) tumors. Four of them had histological gradus G2, and one had gradus G3, while by the (FIGO) classification one was with stage Ib, one was with stage IIIb, while the three were with stage IIIc. LOH in both of the analyzed regions was detected in one tumor (7.7%), with histological gradus G3 and the FIGO IIIc stage. Conclusion. The frequency of LOH in epthelial ovarian carcinomas was 30.7% and 38.5% for p53 and BRCA1 gene regions, respectively. Most of tumors with LOH had histological gradus G2 or G3, and the clinical FIGO stage IIIc, suggesting the association of this occurrence with a later phase of the disease. |
| first_indexed | 2024-12-10T14:07:19Z |
| format | Article |
| id | doaj.art-3e1eecbb843a4a39ad58424f6ba7cd7c |
| institution | Directory Open Access Journal |
| issn | 0042-8450 |
| language | English |
| last_indexed | 2025-03-14T13:04:57Z |
| publishDate | 2006-01-01 |
| publisher | Military Health Department, Ministry of Defence, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-3e1eecbb843a4a39ad58424f6ba7cd7c2025-03-02T01:40:37ZengMilitary Health Department, Ministry of Defence, SerbiaVojnosanitetski Pregled0042-84502006-01-0163981381810.2298/VSP0609813PAnalysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomasLuković LjiljanaPopović BrankaAtanacković JasminaNovaković IvanaPerović MilicaPetrović BojanaPetković SpasojeBackground/aim: Among the genes involved in ovarian carcinogenesis, there has been increased interest in tumor-suppressor genes p53 and BRCA1. Both of the genes make control of cell cycle, DNA repair and apoptosis. The p53 is a "genome guardian" inactivated in more than 50% of human cancers, while BRCA1 mutations are found mostly in breast and ovarian cancer. The aim of this investigation was to establish the frequency of loss of heterozygosity (LOH) in the regions of the genes p53 and BRCA1 in ovarian carcinomas, and to analyze the association of LOH with the disease stage and prognosis. Methods. We analyzed 20 patients with a confirmed diagnosis of epithelilal ovarian carcinoma. DNA for molecular-genetic analysis was extracted from the tumor tissue and blood as normal tissue of each person. Microsatellite markers of the regions of genes p53 and BRCA1 were amplified by PCR method. The determination of allelic status of microsatellites and detection of LOH was performed after PAA gel electroforesis. Results. Both of the analyzed microsatellite markers were informative in 13/20 (65%) cases. In the region of gene p53, LOH was established in 4/13 (30.7%) tumors. One of them had histological gradus G1, one had gradus G2, and two of them had gradus G3, while all were with the International Federation of Gynecology and Obstetrics (FIGO) IIIc stage. In the region of gene BRCA1, LOH was detected in 5/13 (38.5%) tumors. Four of them had histological gradus G2, and one had gradus G3, while by the (FIGO) classification one was with stage Ib, one was with stage IIIb, while the three were with stage IIIc. LOH in both of the analyzed regions was detected in one tumor (7.7%), with histological gradus G3 and the FIGO IIIc stage. Conclusion. The frequency of LOH in epthelial ovarian carcinomas was 30.7% and 38.5% for p53 and BRCA1 gene regions, respectively. Most of tumors with LOH had histological gradus G2 or G3, and the clinical FIGO stage IIIc, suggesting the association of this occurrence with a later phase of the disease.http://www.doiserbia.nb.rs/img/doi/0042-8450/2006/0042-84500609813P.pdfovarian neoplasmsloss of heterozygositygenesp53genesBRCA1 |
| spellingShingle | Luković Ljiljana Popović Branka Atanacković Jasmina Novaković Ivana Perović Milica Petrović Bojana Petković Spasoje Analysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomas Vojnosanitetski Pregled ovarian neoplasms loss of heterozygosity genes p53 genes BRCA1 |
| title | Analysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomas |
| title_full | Analysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomas |
| title_fullStr | Analysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomas |
| title_full_unstemmed | Analysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomas |
| title_short | Analysis of loss of heterozygosity of the tumor suppressor genes p53 and BRCA1 in ovarial carcinomas |
| title_sort | analysis of loss of heterozygosity of the tumor suppressor genes p53 and brca1 in ovarial carcinomas |
| topic | ovarian neoplasms loss of heterozygosity genes p53 genes BRCA1 |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2006/0042-84500609813P.pdf |
| work_keys_str_mv | AT lukovicljiljana analysisoflossofheterozygosityofthetumorsuppressorgenesp53andbrca1inovarialcarcinomas AT popovicbranka analysisoflossofheterozygosityofthetumorsuppressorgenesp53andbrca1inovarialcarcinomas AT atanackovicjasmina analysisoflossofheterozygosityofthetumorsuppressorgenesp53andbrca1inovarialcarcinomas AT novakovicivana analysisoflossofheterozygosityofthetumorsuppressorgenesp53andbrca1inovarialcarcinomas AT perovicmilica analysisoflossofheterozygosityofthetumorsuppressorgenesp53andbrca1inovarialcarcinomas AT petrovicbojana analysisoflossofheterozygosityofthetumorsuppressorgenesp53andbrca1inovarialcarcinomas AT petkovicspasoje analysisoflossofheterozygosityofthetumorsuppressorgenesp53andbrca1inovarialcarcinomas |