Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei
Abstract Background “Bairihua”, a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. Results SPL homologue...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-2303-z |
| _version_ | 1818047057284300800 |
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| author | Zhi Wang Tianqing Zhu Wenjun Ma Erqin Fan Nan Lu Fangqun Ouyang Nan Wang Guijuan Yang Lisheng Kong Guanzheng Qu Shougong Zhang Junhui Wang |
| author_facet | Zhi Wang Tianqing Zhu Wenjun Ma Erqin Fan Nan Lu Fangqun Ouyang Nan Wang Guijuan Yang Lisheng Kong Guanzheng Qu Shougong Zhang Junhui Wang |
| author_sort | Zhi Wang |
| collection | DOAJ |
| description | Abstract Background “Bairihua”, a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. Results SPL homologues CbuSPL9 was cloned using degenerate primers with RACE. Expression studies during flowering transition in “Bairihua” and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in “Bairihua”. Intriguingly, ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time. Conclusions Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA. Further investments need to be done to verify the details of this pathway. |
| first_indexed | 2024-12-10T09:59:44Z |
| format | Article |
| id | doaj.art-3e1fbdd15fb64b37b7468ddec6d06718 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-10T09:59:44Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-3e1fbdd15fb64b37b7468ddec6d067182022-12-22T01:53:23ZengBMCBMC Plant Biology1471-22292020-03-0120111410.1186/s12870-020-2303-zPotential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungeiZhi Wang0Tianqing Zhu1Wenjun Ma2Erqin Fan3Nan Lu4Fangqun Ouyang5Nan Wang6Guijuan Yang7Lisheng Kong8Guanzheng Qu9Shougong Zhang10Junhui Wang11Present address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: Department of Biology Centre for Forest Biology, University of VictoriaPresent address: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryPresent address: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of ForestryAbstract Background “Bairihua”, a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. Results SPL homologues CbuSPL9 was cloned using degenerate primers with RACE. Expression studies during flowering transition in “Bairihua” and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in “Bairihua”. Intriguingly, ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time. Conclusions Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA. Further investments need to be done to verify the details of this pathway.http://link.springer.com/article/10.1186/s12870-020-2303-zCatalpa bungeiFloweringSPLHMGAArchitectural transcriptional factor |
| spellingShingle | Zhi Wang Tianqing Zhu Wenjun Ma Erqin Fan Nan Lu Fangqun Ouyang Nan Wang Guijuan Yang Lisheng Kong Guanzheng Qu Shougong Zhang Junhui Wang Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei BMC Plant Biology Catalpa bungei Flowering SPL HMGA Architectural transcriptional factor |
| title | Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei |
| title_full | Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei |
| title_fullStr | Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei |
| title_full_unstemmed | Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei |
| title_short | Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei |
| title_sort | potential function of cbuspl and gene encoding its interacting protein during flowering in catalpa bungei |
| topic | Catalpa bungei Flowering SPL HMGA Architectural transcriptional factor |
| url | http://link.springer.com/article/10.1186/s12870-020-2303-z |
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