Role of auxin during intercellular infection of Discaria trinervis by Frankia
Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the mo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00399/full |
| _version_ | 1818366939199700992 |
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| author | Leandro eImanishi Leandro eImanishi Francine Manuella Perrine-Walker Adama eNdour Alice eVayssieres Genevieve eConejero Mikael eLucas Antony eChampion Antony eChampion Laurent eLaplaze Laurent eLaplaze Luis eWall Sergio eSVISTOONOFF Sergio eSVISTOONOFF |
| author_facet | Leandro eImanishi Leandro eImanishi Francine Manuella Perrine-Walker Adama eNdour Alice eVayssieres Genevieve eConejero Mikael eLucas Antony eChampion Antony eChampion Laurent eLaplaze Laurent eLaplaze Luis eWall Sergio eSVISTOONOFF Sergio eSVISTOONOFF |
| author_sort | Leandro eImanishi |
| collection | DOAJ |
| description | Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses. |
| first_indexed | 2024-12-13T22:44:07Z |
| format | Article |
| id | doaj.art-3e22894e3b5841078e1233bc67102556 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T22:44:07Z |
| publishDate | 2014-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3e22894e3b5841078e1233bc671025562022-12-21T23:28:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-08-01510.3389/fpls.2014.00399107666Role of auxin during intercellular infection of Discaria trinervis by FrankiaLeandro eImanishi0Leandro eImanishi1Francine Manuella Perrine-Walker2Adama eNdour3Alice eVayssieres4Genevieve eConejero5Mikael eLucas6Antony eChampion7Antony eChampion8Laurent eLaplaze9Laurent eLaplaze10Luis eWall11Sergio eSVISTOONOFF12Sergio eSVISTOONOFF13Institut de Recherche pour le DéveloppementUniversidad Nacional de QuilmesInstitut de Recherche pour le DéveloppementLaboratoire Commun de Microbiologie IRD/ISRA/UCADInstitut de Recherche pour le DéveloppementInatitut National de la Recherche AgronomiqueInstitut de Recherche pour le DéveloppementInstitut de Recherche pour le DéveloppementLaboratoire Commun de Microbiologie IRD/ISRA/UCADInstitut de Recherche pour le DéveloppementLaboratoire Commun de Microbiologie IRD/ISRA/UCADUniversidad Nacional de QuilmesInstitut de Recherche pour le DéveloppementLaboratoire Commun de Microbiologie IRD/ISRA/UCADNitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00399/fullNitrogen FixationSystems BiologyModelauxinendosymbiosisPIN |
| spellingShingle | Leandro eImanishi Leandro eImanishi Francine Manuella Perrine-Walker Adama eNdour Alice eVayssieres Genevieve eConejero Mikael eLucas Antony eChampion Antony eChampion Laurent eLaplaze Laurent eLaplaze Luis eWall Sergio eSVISTOONOFF Sergio eSVISTOONOFF Role of auxin during intercellular infection of Discaria trinervis by Frankia Frontiers in Plant Science Nitrogen Fixation Systems Biology Model auxin endosymbiosis PIN |
| title | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
| title_full | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
| title_fullStr | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
| title_full_unstemmed | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
| title_short | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
| title_sort | role of auxin during intercellular infection of discaria trinervis by frankia |
| topic | Nitrogen Fixation Systems Biology Model auxin endosymbiosis PIN |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00399/full |
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