The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay
Fuel alcohol production yields can be influenced by lignocellulosic biomass loading. High solid loadings (>20 wt%) are suggested to have the potential to produce more products. However, most often, low substrate loadings (<5% solids, <i>w/w</i>) are used to ensure good wett...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/12/18/3526 |
| _version_ | 1828153807084716032 |
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| author | Linda Mezule Ieva Berzina Martins Strods |
| author_facet | Linda Mezule Ieva Berzina Martins Strods |
| author_sort | Linda Mezule |
| collection | DOAJ |
| description | Fuel alcohol production yields can be influenced by lignocellulosic biomass loading. High solid loadings (>20 wt%) are suggested to have the potential to produce more products. However, most often, low substrate loadings (<5% solids, <i>w/w</i>) are used to ensure good wetting and enzyme accessibility, and to minimize any inhibitory effect on the hydrolysis products. Here, we analyzed the effect of substrate loading on the enzymatic hydrolysis of hay with non-commercial enzyme products obtained from white-rot fungi. A significant negative effect on hydrolysis was observed when 10 wt% hay loading was used with the commercial enzyme, however, non-commercial enzyme products from white-rot fungi had no impact on hydrolysis in biomass loading rates from 1 to 10 wt%. Moreover, it was estimated that enzymes extracted from white-rot fungi could be used at a concentration of 0.2 FPU/mL at a biomass loading from 1−10 wt%, resulting in 0.17−0.24 g of released reducing carbohydrates per gram of biomass. Higher concentrations did not result in any significant conversion increase. A mixing impact was only observed in test runs at a substrate loading of 10 wt%. The apparently positive features of the non-commercial enzyme mixes give rise to their future use. The combination and upgrade of existing technologies, e.g., efficient pre-treatment, membrane purification, and concentration and efficient product recovery, should result in even higher conversion yields. |
| first_indexed | 2024-04-11T22:30:05Z |
| format | Article |
| id | doaj.art-3e237d1cf85d4f76bdfb1030e7797d63 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-11T22:30:05Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-3e237d1cf85d4f76bdfb1030e7797d632022-12-22T03:59:26ZengMDPI AGEnergies1996-10732019-09-011218352610.3390/en12183526en12183526The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of HayLinda Mezule0Ieva Berzina1Martins Strods2Water Research Laboratory, Riga Technical University, P. Valdena 1-303 Riga, LatviaWater Research Laboratory, Riga Technical University, P. Valdena 1-303 Riga, LatviaWater Research Laboratory, Riga Technical University, P. Valdena 1-303 Riga, LatviaFuel alcohol production yields can be influenced by lignocellulosic biomass loading. High solid loadings (>20 wt%) are suggested to have the potential to produce more products. However, most often, low substrate loadings (<5% solids, <i>w/w</i>) are used to ensure good wetting and enzyme accessibility, and to minimize any inhibitory effect on the hydrolysis products. Here, we analyzed the effect of substrate loading on the enzymatic hydrolysis of hay with non-commercial enzyme products obtained from white-rot fungi. A significant negative effect on hydrolysis was observed when 10 wt% hay loading was used with the commercial enzyme, however, non-commercial enzyme products from white-rot fungi had no impact on hydrolysis in biomass loading rates from 1 to 10 wt%. Moreover, it was estimated that enzymes extracted from white-rot fungi could be used at a concentration of 0.2 FPU/mL at a biomass loading from 1−10 wt%, resulting in 0.17−0.24 g of released reducing carbohydrates per gram of biomass. Higher concentrations did not result in any significant conversion increase. A mixing impact was only observed in test runs at a substrate loading of 10 wt%. The apparently positive features of the non-commercial enzyme mixes give rise to their future use. The combination and upgrade of existing technologies, e.g., efficient pre-treatment, membrane purification, and concentration and efficient product recovery, should result in even higher conversion yields.https://www.mdpi.com/1996-1073/12/18/3526lignocelluloseenzymatic hydrolysiswhite-rot fungi |
| spellingShingle | Linda Mezule Ieva Berzina Martins Strods The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay Energies lignocellulose enzymatic hydrolysis white-rot fungi |
| title | The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay |
| title_full | The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay |
| title_fullStr | The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay |
| title_full_unstemmed | The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay |
| title_short | The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay |
| title_sort | impact of substrate enzyme proportion for efficient hydrolysis of hay |
| topic | lignocellulose enzymatic hydrolysis white-rot fungi |
| url | https://www.mdpi.com/1996-1073/12/18/3526 |
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