Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro
Osmotic stress is a major factor reducing the growth and yield of many horticultural crops worldwide. To reveal reliable markers of tolerant genotypes, we need a comprehensive understanding of the responsive mechanisms in crops. In vitro stress induction can be an efficient tool to study the mechani...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/12/1795 |
| _version_ | 1797544285799710720 |
|---|---|
| author | Lidiia Samarina Alexandra Matskiv Taisiya Simonyan Natalia Koninskaya Valentina Malyarovskaya Maya Gvasaliya Lyudmila Malyukova Gregory Tsaturyan Alfiya Mytdyeva Marcos Edel Martinez-Montero Ravish Choudhary Alexey Ryndin |
| author_facet | Lidiia Samarina Alexandra Matskiv Taisiya Simonyan Natalia Koninskaya Valentina Malyarovskaya Maya Gvasaliya Lyudmila Malyukova Gregory Tsaturyan Alfiya Mytdyeva Marcos Edel Martinez-Montero Ravish Choudhary Alexey Ryndin |
| author_sort | Lidiia Samarina |
| collection | DOAJ |
| description | Osmotic stress is a major factor reducing the growth and yield of many horticultural crops worldwide. To reveal reliable markers of tolerant genotypes, we need a comprehensive understanding of the responsive mechanisms in crops. In vitro stress induction can be an efficient tool to study the mechanisms of responses in plants to help gain a better understanding of the physiological and genetic responses of plant tissues against each stress factor. In the present study, the osmotic stress was induced by addition of mannitol into the culture media to reveal biochemical and genetic responses of tea microplants. The contents of proline, threonine, epigallocatechin, and epigallocatechin gallate were increased in leaves during mannitol treatment. The expression level of several genes, namely <i>DHN2, LOX1, LOX6, BAM, SUS1, TPS11, RS1, RS2,</i> and <i>SnRK1.3</i>, was elevated by 2–10 times under mannitol-induced osmotic stress, while the expression of many other stress-related genes was not changed significantly. Surprisingly, down-regulation of the following genes, <i>viz</i>. <i>bHLH12, bHLH7, bHLH21, bHLH43, CBF1, WRKY2, SWEET1, SWEET2, SWEET3, INV5,</i> and <i>LOX7,</i> was observed. During this study, two major groups of highly correlated genes were observed. The first group included seven genes, namely <i>CBF1, DHN3, HXK2,</i><i>SnRK1.1, SPS, SWEET3,</i> and <i>SWEET1</i>. The second group comprised eight genes, <i>viz</i>. <i>DHN2, SnRK1.3, HXK3, RS1, RS2,</i><i>LOX6, SUS4,</i> and <i>BAM5</i>. A high level of correlation indicates the high strength connection of the genes which can be co-expressed or can be linked to the joint regulons. The present study demonstrates that tea plants develop several adaptations to cope under osmotic stress in vitro; however, some important stress-related genes were silent or downregulated in microplants. |
| first_indexed | 2024-03-10T13:57:57Z |
| format | Article |
| id | doaj.art-3e2e3630c8544d49b13f7ae4ad63cefd |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T13:57:57Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-3e2e3630c8544d49b13f7ae4ad63cefd2023-11-21T01:22:57ZengMDPI AGPlants2223-77472020-12-01912179510.3390/plants9121795Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In VitroLidiia Samarina0Alexandra Matskiv1Taisiya Simonyan2Natalia Koninskaya3Valentina Malyarovskaya4Maya Gvasaliya5Lyudmila Malyukova6Gregory Tsaturyan7Alfiya Mytdyeva8Marcos Edel Martinez-Montero9Ravish Choudhary10Alexey Ryndin11Federal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaDepartment of Plant Breeding and Plant Conservation, Bioplantas Center, University of Ciego de Avila, Ciego de Avila 65200, CubaDivision of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaFederal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi 354002, RussiaOsmotic stress is a major factor reducing the growth and yield of many horticultural crops worldwide. To reveal reliable markers of tolerant genotypes, we need a comprehensive understanding of the responsive mechanisms in crops. In vitro stress induction can be an efficient tool to study the mechanisms of responses in plants to help gain a better understanding of the physiological and genetic responses of plant tissues against each stress factor. In the present study, the osmotic stress was induced by addition of mannitol into the culture media to reveal biochemical and genetic responses of tea microplants. The contents of proline, threonine, epigallocatechin, and epigallocatechin gallate were increased in leaves during mannitol treatment. The expression level of several genes, namely <i>DHN2, LOX1, LOX6, BAM, SUS1, TPS11, RS1, RS2,</i> and <i>SnRK1.3</i>, was elevated by 2–10 times under mannitol-induced osmotic stress, while the expression of many other stress-related genes was not changed significantly. Surprisingly, down-regulation of the following genes, <i>viz</i>. <i>bHLH12, bHLH7, bHLH21, bHLH43, CBF1, WRKY2, SWEET1, SWEET2, SWEET3, INV5,</i> and <i>LOX7,</i> was observed. During this study, two major groups of highly correlated genes were observed. The first group included seven genes, namely <i>CBF1, DHN3, HXK2,</i><i>SnRK1.1, SPS, SWEET3,</i> and <i>SWEET1</i>. The second group comprised eight genes, <i>viz</i>. <i>DHN2, SnRK1.3, HXK3, RS1, RS2,</i><i>LOX6, SUS4,</i> and <i>BAM5</i>. A high level of correlation indicates the high strength connection of the genes which can be co-expressed or can be linked to the joint regulons. The present study demonstrates that tea plants develop several adaptations to cope under osmotic stress in vitro; however, some important stress-related genes were silent or downregulated in microplants.https://www.mdpi.com/2223-7747/9/12/1795<i>Camellia sinensis</i>in vitroosmotic stressgene expressionmicro-plantsculture media |
| spellingShingle | Lidiia Samarina Alexandra Matskiv Taisiya Simonyan Natalia Koninskaya Valentina Malyarovskaya Maya Gvasaliya Lyudmila Malyukova Gregory Tsaturyan Alfiya Mytdyeva Marcos Edel Martinez-Montero Ravish Choudhary Alexey Ryndin Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro Plants <i>Camellia sinensis</i> in vitro osmotic stress gene expression micro-plants culture media |
| title | Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro |
| title_full | Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro |
| title_fullStr | Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro |
| title_full_unstemmed | Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro |
| title_short | Biochemical and Genetic Responses of Tea (<i>Camellia sinensis</i> (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro |
| title_sort | biochemical and genetic responses of tea i camellia sinensis i l kuntze microplants under mannitol induced osmotic stress in vitro |
| topic | <i>Camellia sinensis</i> in vitro osmotic stress gene expression micro-plants culture media |
| url | https://www.mdpi.com/2223-7747/9/12/1795 |
| work_keys_str_mv | AT lidiiasamarina biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT alexandramatskiv biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT taisiyasimonyan biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT nataliakoninskaya biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT valentinamalyarovskaya biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT mayagvasaliya biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT lyudmilamalyukova biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT gregorytsaturyan biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT alfiyamytdyeva biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT marcosedelmartinezmontero biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT ravishchoudhary biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro AT alexeyryndin biochemicalandgeneticresponsesofteaicamelliasinensisilkuntzemicroplantsundermannitolinducedosmoticstressinvitro |